Academic Journal

Efficient Conversion of Urea-Based Non-Protein Nitrogen to Microbial Protein: Mechanisms, Regulation and Industrial Prospects.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Efficient Conversion of Urea-Based Non-Protein Nitrogen to Microbial Protein: Mechanisms, Regulation and Industrial Prospects.
Συγγραφείς: Wang, Bing, Gao, Jingyan, Zhang, Furan, Luan, Jian
Πηγή: Fermentation (Basel); Jul2026, Vol. 12 Issue 7, p331, 16p
Θεματικοί όροι: Nitrogen compounds, Microbial metabolism, Feed industry, Industrial applications, Bacterial proteins, Fermentation, Nitrogen excretion
Περίληψη: Possessing a high nitrogen content, low cost and stable supply, urea serves as a critical non-protein nitrogen (NPN) source that represents a promising alternative to conventional protein feedstocks for reducing production costs in microbial protein (MP) synthesis. This article systematically discusses the application potential of urea in MP production, the metabolic pathways governing microbial urea utilization, and the key factors influencing the conversion efficiency of urea to microbial protein. Herein, we summarize recent progress in urea modification technologies covering slow-release urea, extruded urea, and urea-based composite preparations and elucidate the complete metabolic mechanisms of urea assimilation in both rumen fermentation and in vitro cultivation, including transmembrane transport, urease-catalyzed hydrolysis, ammonia assimilation, and MP synthesis. Furthermore, we analyze the regulatory effects of dietary energy level, carbohydrate structure, protein concentration, forage quality, urea formulation type, and functional additives on nitrogen utilization efficiency. Current evidence indicates substantial knowledge gaps regarding urea transporter functionality, the coordinated regulation of UC and nitrogen assimilation pathways, microbial community interactions, and the stability control of industrial-scale production. The development of high-efficiency utilization technologies, feed safety evaluation systems, and precision feeding models for industrial applications remains incomplete. This article seeks to provide a thorough theoretical reference for the efficient utilization, mechanistic elucidation, and industrial promotion of urea-based nitrogen sources within MP production systems. [ABSTRACT FROM AUTHOR]
Copyright of Fermentation (Basel) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Βάση Δεδομένων: Complementary Index
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  Label: Title
  Group: Ti
  Data: Efficient Conversion of Urea-Based Non-Protein Nitrogen to Microbial Protein: Mechanisms, Regulation and Industrial Prospects.
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  Data: <searchLink fieldCode="AR" term="%22Wang%2C+Bing%22">Wang, Bing</searchLink><br /><searchLink fieldCode="AR" term="%22Gao%2C+Jingyan%22">Gao, Jingyan</searchLink><br /><searchLink fieldCode="AR" term="%22Zhang%2C+Furan%22">Zhang, Furan</searchLink><br /><searchLink fieldCode="AR" term="%22Luan%2C+Jian%22">Luan, Jian</searchLink>
– Name: TitleSource
  Label: Source
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  Data: Fermentation (Basel); Jul2026, Vol. 12 Issue 7, p331, 16p
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Nitrogen+compounds%22">Nitrogen compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Microbial+metabolism%22">Microbial metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Feed+industry%22">Feed industry</searchLink><br /><searchLink fieldCode="DE" term="%22Industrial+applications%22">Industrial applications</searchLink><br /><searchLink fieldCode="DE" term="%22Bacterial+proteins%22">Bacterial proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Fermentation%22">Fermentation</searchLink><br /><searchLink fieldCode="DE" term="%22Nitrogen+excretion%22">Nitrogen excretion</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Possessing a high nitrogen content, low cost and stable supply, urea serves as a critical non-protein nitrogen (NPN) source that represents a promising alternative to conventional protein feedstocks for reducing production costs in microbial protein (MP) synthesis. This article systematically discusses the application potential of urea in MP production, the metabolic pathways governing microbial urea utilization, and the key factors influencing the conversion efficiency of urea to microbial protein. Herein, we summarize recent progress in urea modification technologies covering slow-release urea, extruded urea, and urea-based composite preparations and elucidate the complete metabolic mechanisms of urea assimilation in both rumen fermentation and in vitro cultivation, including transmembrane transport, urease-catalyzed hydrolysis, ammonia assimilation, and MP synthesis. Furthermore, we analyze the regulatory effects of dietary energy level, carbohydrate structure, protein concentration, forage quality, urea formulation type, and functional additives on nitrogen utilization efficiency. Current evidence indicates substantial knowledge gaps regarding urea transporter functionality, the coordinated regulation of UC and nitrogen assimilation pathways, microbial community interactions, and the stability control of industrial-scale production. The development of high-efficiency utilization technologies, feed safety evaluation systems, and precision feeding models for industrial applications remains incomplete. This article seeks to provide a thorough theoretical reference for the efficient utilization, mechanistic elucidation, and industrial promotion of urea-based nitrogen sources within MP production systems. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Fermentation (Basel) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.3390/fermentation12070331
    Languages:
      – Code: eng
        Text: English
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        PageCount: 16
        StartPage: 331
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      – SubjectFull: Nitrogen compounds
        Type: general
      – SubjectFull: Microbial metabolism
        Type: general
      – SubjectFull: Feed industry
        Type: general
      – SubjectFull: Industrial applications
        Type: general
      – SubjectFull: Bacterial proteins
        Type: general
      – SubjectFull: Fermentation
        Type: general
      – SubjectFull: Nitrogen excretion
        Type: general
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      – TitleFull: Efficient Conversion of Urea-Based Non-Protein Nitrogen to Microbial Protein: Mechanisms, Regulation and Industrial Prospects.
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            NameFull: Gao, Jingyan
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            – D: 01
              M: 07
              Text: Jul2026
              Type: published
              Y: 2026
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              Value: 12
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