Academic Journal
A TCER-1-siRNA regulatory axis suppresses antibacterial innate immunity in C. elegans.
| Τίτλος: | A TCER-1-siRNA regulatory axis suppresses antibacterial innate immunity in C. elegans. |
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| Συγγραφείς: | Naim, Nikki, Amrit, Francis R.G., Devare, Mayur N., Osman, Guled A., Bahr, Laura L., Henry, Hannah, Montgomery, Brooke E., Kuhn, Spencer M., Montgomery, Taiowa A., Ghazi, Arjumand |
| Πηγή: | PLoS Pathogens; 7/28/2026, Vol. 22 Issue 7, p1-14, 14p |
| Θεματικοί όροι: | Small interfering RNA, Caenorhabditis elegans, Natural immunity |
| Περίληψη: | Small interfering RNAs (siRNAs) are important regulators of gene expression with well-established roles in pathogen defense. Yet, their specific roles in antibacterial immunity are not well understood. Here, we identify an siRNA pathway involved in repressing antibacterial innate immunity in Caenorhabditis elegans. We show that genes required for the biogenesis or function of WAGO Argonaute-associated siRNAs, called 22G-RNAs, function in a common genetic pathway with the immune-suppressive transcription elongation and splicing factor TCER-1 to inhibit immunity. Loss of tcer-1 led to reduced levels of 22G-RNAs from a subset of WAGO targets, while mutations in several WAGO 22G-RNA pathway genes phenocopied the enhanced immunoresistance of tcer-1 mutants, suggesting a shared regulatory module. Integrative 22G-RNA-mRNA analyses and molecular genetic studies show that this module does not induce widespread gene silencing, but instead targets a restricted set of immune-relevant effectors, including scrm-4, which encodes a conserved phospholipid translocase that promotes host resistance. Together, our findings establish endogenous WAGO 22G-RNAs as repressors of antibacterial immunity and identify TCER-1 as a physiological regulator that promotes 22G-RNA biogenesis to constrain host defense. The results uncover a previously unrecognized small RNA-dependent mechanism linking transcription, metabolism, and antibacterial innate immunity. Author summary: Small interfering RNAs (siRNAs) are recognized as having critical roles in controlling how genes and proteins involved in diverse biological processes are expressed, including during immune response following viral infections. But, their role in immunity against bacterial pathogens is not clear. We provide evidence for an siRNA pathway, regulated by the C. elegans protein TCER-1, that represses antibacterial innate immunity. TCER-1, along with WAGO Argonaute proteins involved in 22G-RNA biogenesis, promotes the production of a subset of 22G-RNAs that repress a select group of targets, including a lipid-modifying scramblase enzyme, SCRM-4, that is necessary for immune activation. This results reveals a novel small RNA-dependent mechanism of antibacterial innate immunity. [ABSTRACT FROM AUTHOR] |
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| Βάση Δεδομένων: | Complementary Index |
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| Items | – Name: Title Label: Title Group: Ti Data: A TCER-1-siRNA regulatory axis suppresses antibacterial innate immunity in C. elegans. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Naim%2C+Nikki%22">Naim, Nikki</searchLink><br /><searchLink fieldCode="AR" term="%22Amrit%2C+Francis+R%2EG%2E%22">Amrit, Francis R.G.</searchLink><br /><searchLink fieldCode="AR" term="%22Devare%2C+Mayur+N%2E%22">Devare, Mayur N.</searchLink><br /><searchLink fieldCode="AR" term="%22Osman%2C+Guled+A%2E%22">Osman, Guled A.</searchLink><br /><searchLink fieldCode="AR" term="%22Bahr%2C+Laura+L%2E%22">Bahr, Laura L.</searchLink><br /><searchLink fieldCode="AR" term="%22Henry%2C+Hannah%22">Henry, Hannah</searchLink><br /><searchLink fieldCode="AR" term="%22Montgomery%2C+Brooke+E%2E%22">Montgomery, Brooke E.</searchLink><br /><searchLink fieldCode="AR" term="%22Kuhn%2C+Spencer+M%2E%22">Kuhn, Spencer M.</searchLink><br /><searchLink fieldCode="AR" term="%22Montgomery%2C+Taiowa+A%2E%22">Montgomery, Taiowa A.</searchLink><br /><searchLink fieldCode="AR" term="%22Ghazi%2C+Arjumand%22">Ghazi, Arjumand</searchLink> – Name: TitleSource Label: Source Group: Src Data: PLoS Pathogens; 7/28/2026, Vol. 22 Issue 7, p1-14, 14p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Small+interfering+RNA%22">Small interfering RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Caenorhabditis+elegans%22">Caenorhabditis elegans</searchLink><br /><searchLink fieldCode="DE" term="%22Natural+immunity%22">Natural immunity</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Small interfering RNAs (siRNAs) are important regulators of gene expression with well-established roles in pathogen defense. Yet, their specific roles in antibacterial immunity are not well understood. Here, we identify an siRNA pathway involved in repressing antibacterial innate immunity in Caenorhabditis elegans. We show that genes required for the biogenesis or function of WAGO Argonaute-associated siRNAs, called 22G-RNAs, function in a common genetic pathway with the immune-suppressive transcription elongation and splicing factor TCER-1 to inhibit immunity. Loss of tcer-1 led to reduced levels of 22G-RNAs from a subset of WAGO targets, while mutations in several WAGO 22G-RNA pathway genes phenocopied the enhanced immunoresistance of tcer-1 mutants, suggesting a shared regulatory module. Integrative 22G-RNA-mRNA analyses and molecular genetic studies show that this module does not induce widespread gene silencing, but instead targets a restricted set of immune-relevant effectors, including scrm-4, which encodes a conserved phospholipid translocase that promotes host resistance. Together, our findings establish endogenous WAGO 22G-RNAs as repressors of antibacterial immunity and identify TCER-1 as a physiological regulator that promotes 22G-RNA biogenesis to constrain host defense. The results uncover a previously unrecognized small RNA-dependent mechanism linking transcription, metabolism, and antibacterial innate immunity. Author summary: Small interfering RNAs (siRNAs) are recognized as having critical roles in controlling how genes and proteins involved in diverse biological processes are expressed, including during immune response following viral infections. But, their role in immunity against bacterial pathogens is not clear. We provide evidence for an siRNA pathway, regulated by the C. elegans protein TCER-1, that represses antibacterial innate immunity. TCER-1, along with WAGO Argonaute proteins involved in 22G-RNA biogenesis, promotes the production of a subset of 22G-RNAs that repress a select group of targets, including a lipid-modifying scramblase enzyme, SCRM-4, that is necessary for immune activation. This results reveals a novel small RNA-dependent mechanism of antibacterial innate immunity. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of PLoS Pathogens is the property of Public Library of Science and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1371/journal.ppat.1013972 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 14 StartPage: 1 Subjects: – SubjectFull: Small interfering RNA Type: general – SubjectFull: Caenorhabditis elegans Type: general – SubjectFull: Natural immunity Type: general Titles: – TitleFull: A TCER-1-siRNA regulatory axis suppresses antibacterial innate immunity in C. elegans. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Naim, Nikki – PersonEntity: Name: NameFull: Amrit, Francis R.G. – PersonEntity: Name: NameFull: Devare, Mayur N. – PersonEntity: Name: NameFull: Osman, Guled A. – PersonEntity: Name: NameFull: Bahr, Laura L. – PersonEntity: Name: NameFull: Henry, Hannah – PersonEntity: Name: NameFull: Montgomery, Brooke E. – PersonEntity: Name: NameFull: Kuhn, Spencer M. – PersonEntity: Name: NameFull: Montgomery, Taiowa A. – PersonEntity: Name: NameFull: Ghazi, Arjumand IsPartOfRelationships: – BibEntity: Dates: – D: 28 M: 07 Text: 7/28/2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 15537366 Numbering: – Type: volume Value: 22 – Type: issue Value: 7 Titles: – TitleFull: PLoS Pathogens Type: main |
| ResultId | 1 |