Academic Journal
Associations of Mitochondrial Heteroplasmy in Blood Monocytes with Its Phenotypic Indices and Cardiometabolic Parameters in Metabolic Syndrome: A Pilot Cross-sectional Study.
| Τίτλος: | Associations of Mitochondrial Heteroplasmy in Blood Monocytes with Its Phenotypic Indices and Cardiometabolic Parameters in Metabolic Syndrome: A Pilot Cross-sectional Study. |
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| Συγγραφείς: | Voronova, Sophia, Bograya, Maria, Koshel, Anastasia, Malashchenko, Vladimir, Vulf, Maria, Beletskaya, Maria, Litvinova, Larisa |
| Πηγή: | Gene Expression (1052-2166); Apr2026, Vol. 25 Issue 2, p1-13, 13p |
| Θεματικοί όροι: | Mitochondrial DNA, Metabolic syndrome, Cytokines, Gene expression, Monocytes |
| Περίληψη: | Background and objectives: Mitochondrial DNA (mtDNA) variability, especially heteroplasmy, is believed to affect cellular immunobiogenesis, particularly in monocytes in metabolic syndrome (MetS). This study aimed to identify associations of monocytic mtDNA variability with its phenotypic indices, including cytokine secretion and gene expression, and cardiometabolic parameters in patients with MetS. Methods: The cross-sectional study recruited 87 adult participants, including 34 healthy blood donors (Control group), 21 obese patients (Obesity group), and 32 MetS patients (MetS group). Blood biochemistry tests were performed on venous blood samples, and monocytes (CD14+ cells) were isolated. Monocyte mtDNA was analyzed by next-generation sequencing to identify low (5–10%) and intermediate (10–95%) heteroplasmy, and homoplasmy (≥95%). Expression of genes related to mitochondrial biogenesis, mitochondrial uncoupling, oxidative stress system, and NF-κB signaling was assessed by quantitative real-time polymerase chain reaction. Monocytes cultured with and without lipopolysaccharide for 24 h were analyzed by enzyme-linked immunosorbent assay to assess the cytokine secretion stimulation index. Results: Monocyte mtDNA showed low variability, but alternative homoplasmies were significantly more common. Intermediate and low heteroplasmy from the protein-coding locus correlated with stenosis (r = 0.396; 95% confidence interval (CI) 0.067–0.647) and low-density lipoprotein levels (r = −0.258; 95% CI −0.45 – −0.043). Intermediate heteroplasmy from the rRNA locus correlated with blood insulin levels (r = −0.228; 95% CI −0.424 – −0.019). D-loop low heteroplasmy correlated with fasting blood glucose (r = 0.275; 95% CI 0.062–0.464). Homoplasmies were associated with creatinine, blood urea nitrogen, and alkaline phosphatase. Intermediate heteroplasmy in mtDNA was associated with the monocyte cytokine secretion stimulation index (R² = 0.156, P = 0.003). However, there was no significant association between mtDNA variability and the expression of the various genes. Conclusions: Monocyte mtDNA shows relatively low variability. Low and Intermediate heteroplasmy are associated with cardiometabolic parameters, and intermediate heteroplasmy is associated with the monocyte cytokine secretion stimulation index. [ABSTRACT FROM AUTHOR] |
| Copyright of Gene Expression (1052-2166) is the property of Xia & He Publishing Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Βάση Δεδομένων: | Complementary Index |
| FullText | Text: Availability: 0 |
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| Header | DbId: edb DbLabel: Complementary Index An: 194016311 RelevancyScore: 1061 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1060.75964355469 |
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| Items | – Name: Title Label: Title Group: Ti Data: Associations of Mitochondrial Heteroplasmy in Blood Monocytes with Its Phenotypic Indices and Cardiometabolic Parameters in Metabolic Syndrome: A Pilot Cross-sectional Study. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Voronova%2C+Sophia%22">Voronova, Sophia</searchLink><br /><searchLink fieldCode="AR" term="%22Bograya%2C+Maria%22">Bograya, Maria</searchLink><br /><searchLink fieldCode="AR" term="%22Koshel%2C+Anastasia%22">Koshel, Anastasia</searchLink><br /><searchLink fieldCode="AR" term="%22Malashchenko%2C+Vladimir%22">Malashchenko, Vladimir</searchLink><br /><searchLink fieldCode="AR" term="%22Vulf%2C+Maria%22">Vulf, Maria</searchLink><br /><searchLink fieldCode="AR" term="%22Beletskaya%2C+Maria%22">Beletskaya, Maria</searchLink><br /><searchLink fieldCode="AR" term="%22Litvinova%2C+Larisa%22">Litvinova, Larisa</searchLink> – Name: TitleSource Label: Source Group: Src Data: Gene Expression (1052-2166); Apr2026, Vol. 25 Issue 2, p1-13, 13p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Mitochondrial+DNA%22">Mitochondrial DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolic+syndrome%22">Metabolic syndrome</searchLink><br /><searchLink fieldCode="DE" term="%22Cytokines%22">Cytokines</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Monocytes%22">Monocytes</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Background and objectives: Mitochondrial DNA (mtDNA) variability, especially heteroplasmy, is believed to affect cellular immunobiogenesis, particularly in monocytes in metabolic syndrome (MetS). This study aimed to identify associations of monocytic mtDNA variability with its phenotypic indices, including cytokine secretion and gene expression, and cardiometabolic parameters in patients with MetS. Methods: The cross-sectional study recruited 87 adult participants, including 34 healthy blood donors (Control group), 21 obese patients (Obesity group), and 32 MetS patients (MetS group). Blood biochemistry tests were performed on venous blood samples, and monocytes (CD14<superscript>+</superscript> cells) were isolated. Monocyte mtDNA was analyzed by next-generation sequencing to identify low (5–10%) and intermediate (10–95%) heteroplasmy, and homoplasmy (≥95%). Expression of genes related to mitochondrial biogenesis, mitochondrial uncoupling, oxidative stress system, and NF-κB signaling was assessed by quantitative real-time polymerase chain reaction. Monocytes cultured with and without lipopolysaccharide for 24 h were analyzed by enzyme-linked immunosorbent assay to assess the cytokine secretion stimulation index. Results: Monocyte mtDNA showed low variability, but alternative homoplasmies were significantly more common. Intermediate and low heteroplasmy from the protein-coding locus correlated with stenosis (r = 0.396; 95% confidence interval (CI) 0.067–0.647) and low-density lipoprotein levels (r = −0.258; 95% CI −0.45 – −0.043). Intermediate heteroplasmy from the rRNA locus correlated with blood insulin levels (r = −0.228; 95% CI −0.424 – −0.019). D-loop low heteroplasmy correlated with fasting blood glucose (r = 0.275; 95% CI 0.062–0.464). Homoplasmies were associated with creatinine, blood urea nitrogen, and alkaline phosphatase. Intermediate heteroplasmy in mtDNA was associated with the monocyte cytokine secretion stimulation index (R² = 0.156, P = 0.003). However, there was no significant association between mtDNA variability and the expression of the various genes. Conclusions: Monocyte mtDNA shows relatively low variability. Low and Intermediate heteroplasmy are associated with cardiometabolic parameters, and intermediate heteroplasmy is associated with the monocyte cytokine secretion stimulation index. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Gene Expression (1052-2166) is the property of Xia & He Publishing Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.14218/GE.2025.00088 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 1 Subjects: – SubjectFull: Mitochondrial DNA Type: general – SubjectFull: Metabolic syndrome Type: general – SubjectFull: Cytokines Type: general – SubjectFull: Gene expression Type: general – SubjectFull: Monocytes Type: general Titles: – TitleFull: Associations of Mitochondrial Heteroplasmy in Blood Monocytes with Its Phenotypic Indices and Cardiometabolic Parameters in Metabolic Syndrome: A Pilot Cross-sectional Study. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Voronova, Sophia – PersonEntity: Name: NameFull: Bograya, Maria – PersonEntity: Name: NameFull: Koshel, Anastasia – PersonEntity: Name: NameFull: Malashchenko, Vladimir – PersonEntity: Name: NameFull: Vulf, Maria – PersonEntity: Name: NameFull: Beletskaya, Maria – PersonEntity: Name: NameFull: Litvinova, Larisa IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 10522166 Numbering: – Type: volume Value: 25 – Type: issue Value: 2 Titles: – TitleFull: Gene Expression (1052-2166) Type: main |
| ResultId | 1 |