Academic Journal
Advances in primary cell isolation, sorting, and preservation from livestock for cultured meat applications.
| Τίτλος: | Advances in primary cell isolation, sorting, and preservation from livestock for cultured meat applications. |
|---|---|
| Συγγραφείς: | Park, Sanghun, Park, Gyutae, Oh, Sehyuk, Lee, Sol-Hee, Choi, Jungseok |
| Πηγή: | Critical Reviews in Biotechnology; May2026, Vol. 46 Issue 3, p522-540, 19p |
| Θεματικοί όροι: | Cell separation, Cell preservation, Cryopreservation of organs, tissues, etc., Animal cell biotechnology, Biochemical engineering, Cell fractionation, In vitro meat |
| Περίληψη: | Cultured meat production relies on the efficient: isolation, purification, expansion, and preservation of livestock-derived primary cells with robust proliferation and differentiation potential. Satellite cells and fibro-adipogenic progenitors from muscle and adipose tissues are key cellular sources for the in vitro reconstruction of meat-like structures. This review critically examines current methodologies used for primary cell processing in cultured meat scalable bioprocessing research. Tissue dissociation strategies are classified as enzymatic, non-enzymatic, and hybrid protocols and compared in terms of yield, scalability, and preservation of cell functionality. Cell purification techniques include: density gradient centrifugation, pre-plating, fluorescence-activated cell sorting (FACS), and magnetic-activated cell sorting (MACS), each offering different levels of specificity, throughput, and compatibility with food-grade applications. Long-term storage via cryopreservation is essential for establishing reliable cell banks. However, challenges remain in minimizing cryoinjury, selecting optimal cryoprotective agents, and maintaining post-thaw viability. Emerging alternatives, such as temperature-responsive cell sheet technology and biochemical protection strategies, are highlighted for their potential to improve cell recovery and functionality. Unlike existing reviews that often focus on isolated aspects, this article integrates dissociation, purification, and preservation into one framework. It highlights how optimized protocols can overcome current bottlenecks in cell processing. This review emphasizes the importance of standardized and food-safe platforms. Such advances will support commercialization and sustainable delivery of cultured meat products. STATEMENT OF SIGNIFICANCE: This review addresses a critical challenge in cultured meat production: the lack of integrated, food-safe strategies for primary cell processing. Unlike previous reviews that examined cell isolation, sorting, or preservation in isolation, it unifies these steps into a single framework that balances biological performance with scalability and safety. By linking advanced cell handling technologies to reproducibility and commercial feasibility, the review provides actionable insights for both researchers and industry, supporting the development of sustainable and reliable cultured meat systems. [ABSTRACT FROM AUTHOR] |
| Copyright of Critical Reviews in Biotechnology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Βάση Δεδομένων: | Complementary Index |
| FullText | Text: Availability: 0 |
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| Header | DbId: edb DbLabel: Complementary Index An: 193014440 RelevancyScore: 1061 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1060.76245117188 |
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| Items | – Name: Title Label: Title Group: Ti Data: Advances in primary cell isolation, sorting, and preservation from livestock for cultured meat applications. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Park%2C+Sanghun%22">Park, Sanghun</searchLink><br /><searchLink fieldCode="AR" term="%22Park%2C+Gyutae%22">Park, Gyutae</searchLink><br /><searchLink fieldCode="AR" term="%22Oh%2C+Sehyuk%22">Oh, Sehyuk</searchLink><br /><searchLink fieldCode="AR" term="%22Lee%2C+Sol-Hee%22">Lee, Sol-Hee</searchLink><br /><searchLink fieldCode="AR" term="%22Choi%2C+Jungseok%22">Choi, Jungseok</searchLink> – Name: TitleSource Label: Source Group: Src Data: Critical Reviews in Biotechnology; May2026, Vol. 46 Issue 3, p522-540, 19p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Cell+separation%22">Cell separation</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+preservation%22">Cell preservation</searchLink><br /><searchLink fieldCode="DE" term="%22Cryopreservation+of+organs%2C+tissues%2C+etc%2E%22">Cryopreservation of organs, tissues, etc.</searchLink><br /><searchLink fieldCode="DE" term="%22Animal+cell+biotechnology%22">Animal cell biotechnology</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemical+engineering%22">Biochemical engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+fractionation%22">Cell fractionation</searchLink><br /><searchLink fieldCode="DE" term="%22In+vitro+meat%22">In vitro meat</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Cultured meat production relies on the efficient: isolation, purification, expansion, and preservation of livestock-derived primary cells with robust proliferation and differentiation potential. Satellite cells and fibro-adipogenic progenitors from muscle and adipose tissues are key cellular sources for the in vitro reconstruction of meat-like structures. This review critically examines current methodologies used for primary cell processing in cultured meat scalable bioprocessing research. Tissue dissociation strategies are classified as enzymatic, non-enzymatic, and hybrid protocols and compared in terms of yield, scalability, and preservation of cell functionality. Cell purification techniques include: density gradient centrifugation, pre-plating, fluorescence-activated cell sorting (FACS), and magnetic-activated cell sorting (MACS), each offering different levels of specificity, throughput, and compatibility with food-grade applications. Long-term storage via cryopreservation is essential for establishing reliable cell banks. However, challenges remain in minimizing cryoinjury, selecting optimal cryoprotective agents, and maintaining post-thaw viability. Emerging alternatives, such as temperature-responsive cell sheet technology and biochemical protection strategies, are highlighted for their potential to improve cell recovery and functionality. Unlike existing reviews that often focus on isolated aspects, this article integrates dissociation, purification, and preservation into one framework. It highlights how optimized protocols can overcome current bottlenecks in cell processing. This review emphasizes the importance of standardized and food-safe platforms. Such advances will support commercialization and sustainable delivery of cultured meat products. STATEMENT OF SIGNIFICANCE: This review addresses a critical challenge in cultured meat production: the lack of integrated, food-safe strategies for primary cell processing. Unlike previous reviews that examined cell isolation, sorting, or preservation in isolation, it unifies these steps into a single framework that balances biological performance with scalability and safety. By linking advanced cell handling technologies to reproducibility and commercial feasibility, the review provides actionable insights for both researchers and industry, supporting the development of sustainable and reliable cultured meat systems. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Critical Reviews in Biotechnology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1080/07388551.2026.2618192 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 19 StartPage: 522 Subjects: – SubjectFull: Cell separation Type: general – SubjectFull: Cell preservation Type: general – SubjectFull: Cryopreservation of organs, tissues, etc. Type: general – SubjectFull: Animal cell biotechnology Type: general – SubjectFull: Biochemical engineering Type: general – SubjectFull: Cell fractionation Type: general – SubjectFull: In vitro meat Type: general Titles: – TitleFull: Advances in primary cell isolation, sorting, and preservation from livestock for cultured meat applications. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Park, Sanghun – PersonEntity: Name: NameFull: Park, Gyutae – PersonEntity: Name: NameFull: Oh, Sehyuk – PersonEntity: Name: NameFull: Lee, Sol-Hee – PersonEntity: Name: NameFull: Choi, Jungseok IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Text: May2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 07388551 Numbering: – Type: volume Value: 46 – Type: issue Value: 3 Titles: – TitleFull: Critical Reviews in Biotechnology Type: main |
| ResultId | 1 |