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Chitosan-mediated nano-bioprocessing of Acacia seyal seed extract for enhanced antineoplastic, anti-Helicobacter pylori and antioxidant performance.

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Title: Chitosan-mediated nano-bioprocessing of Acacia seyal seed extract for enhanced antineoplastic, anti-Helicobacter pylori and antioxidant performance.
Authors: Al-Rajhi, Aisha M. H., Mohamed, Marwa Yousry A., Mothana, Areej, Debaji, Abdulaziz, Ganash, Magdah, Ali, Yahya, Alharbi, Asmaa A., Abdelghany, Tarek M.
Source: Bioresources & Bioprocessing; 4/2/2026, Vol. 13 Issue 1, p1-15, 15p
Subject Terms: Nanoparticles, Antineoplastic agents, Molecular docking, Nanocapsules, Acacia, Antioxidants, Phytochemicals, Helicobacter pylori
Abstract: Acacia seyal is a medicinal plant rich in bioactive compounds known for their antimicrobial, antioxidant, and anticancer properties. Conventional plant extracts often suffer from poor stability and limited bioavailability, reducing their therapeutic effectiveness. The utilization of chitosan nanoparticles (CS-NPs) offers a promising strategy to enhance biological activity of plant extracts. Encapsulation of A. seyal seeds extract (ASSE) in CS-NPs (ASSE@CS-NPs) was the aim of the present investigation to compete Helicobacter pylori and HCT116 cancer cells. The phytochemical profile of ASSE revealed diverse phenolic acids and flavonoids via HPLC analysis. Methyl gallate was dominated (20.98 mg/g), followed by gallic acid, and catechin. FTIR analysis confirmed characteristic functional groups in ASSE and CS-NPs. Peak shifts and intensity variations in ASSE@CS-NPs indicated successful encapsulation through hydrogen bonding and electrostatic interactions. ASSE@CS-NPs showed the strongest anti-H. pylori effect with inhibition zone (23.7 mm) and lowest MIC/MBC (15.62 µg/mL). Compared to CS-NPs (20.7 mm, 31.25 µg/mL) and ASSE (19.7 mm, 31.25 µg/mL). ASSE@CS-NPs showed notable antioxidant activity, achieving 96% scavenging at 1000 µg/mL with IC50 of 3µg/mL compared to ASSE (5 µg/mL) and CS-NPs (74 µg/mL). ASSE@CS-NPs exhibited potent cytotoxicity against HCT116 cells with IC50 of 23 µg/mL, outperforming ASSE (588 µg/mL) and CS-NPs (120 µg/mL). The formulation achieved > 80% inhibition at 62.5 µg/mL. Molecular docking was performed to evaluate the binding affinity and interaction patterns of methyl gallate (a main constituent of ASSE) and chitosan against H. pylori urease (PDB ID: 6ZJA). Docking results revealed favorable binding scores for both ligands, with chitosan showing higher affinity (S score: − 6.42 kcal/mol) compared to methyl gallate (S score: − 5.45 kcal/mol). Key hydrogen bond interactions were observed with active site residues ASP362 and ALA169 for methyl gallate, and ASP223 and HIS323 for chitosan. These results suggest that ASSE@CS-NPs possess inhibitory potential against H. pylori and HCT116 cells. [ABSTRACT FROM AUTHOR]
Copyright of Bioresources & Bioprocessing is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  – Url: https://dx.doi.org/doi:10.1186/s40643-026-01031-6
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  Data: Chitosan-mediated nano-bioprocessing of Acacia seyal seed extract for enhanced antineoplastic, anti-Helicobacter pylori and antioxidant performance.
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  Data: Bioresources & Bioprocessing; 4/2/2026, Vol. 13 Issue 1, p1-15, 15p
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  Data: <searchLink fieldCode="DE" term="%22Nanoparticles%22">Nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Antineoplastic+agents%22">Antineoplastic agents</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+docking%22">Molecular docking</searchLink><br /><searchLink fieldCode="DE" term="%22Nanocapsules%22">Nanocapsules</searchLink><br /><searchLink fieldCode="DE" term="%22Acacia%22">Acacia</searchLink><br /><searchLink fieldCode="DE" term="%22Antioxidants%22">Antioxidants</searchLink><br /><searchLink fieldCode="DE" term="%22Phytochemicals%22">Phytochemicals</searchLink><br /><searchLink fieldCode="DE" term="%22Helicobacter+pylori%22">Helicobacter pylori</searchLink>
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  Label: Abstract
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  Data: Acacia seyal is a medicinal plant rich in bioactive compounds known for their antimicrobial, antioxidant, and anticancer properties. Conventional plant extracts often suffer from poor stability and limited bioavailability, reducing their therapeutic effectiveness. The utilization of chitosan nanoparticles (CS-NPs) offers a promising strategy to enhance biological activity of plant extracts. Encapsulation of A. seyal seeds extract (ASSE) in CS-NPs (ASSE@CS-NPs) was the aim of the present investigation to compete Helicobacter pylori and HCT116 cancer cells. The phytochemical profile of ASSE revealed diverse phenolic acids and flavonoids via HPLC analysis. Methyl gallate was dominated (20.98 mg/g), followed by gallic acid, and catechin. FTIR analysis confirmed characteristic functional groups in ASSE and CS-NPs. Peak shifts and intensity variations in ASSE@CS-NPs indicated successful encapsulation through hydrogen bonding and electrostatic interactions. ASSE@CS-NPs showed the strongest anti-H. pylori effect with inhibition zone (23.7 mm) and lowest MIC/MBC (15.62 µg/mL). Compared to CS-NPs (20.7 mm, 31.25 µg/mL) and ASSE (19.7 mm, 31.25 µg/mL). ASSE@CS-NPs showed notable antioxidant activity, achieving 96% scavenging at 1000 µg/mL with IC<subscript>50</subscript> of 3µg/mL compared to ASSE (5 µg/mL) and CS-NPs (74 µg/mL). ASSE@CS-NPs exhibited potent cytotoxicity against HCT116 cells with IC<subscript>50</subscript> of 23 µg/mL, outperforming ASSE (588 µg/mL) and CS-NPs (120 µg/mL). The formulation achieved > 80% inhibition at 62.5 µg/mL. Molecular docking was performed to evaluate the binding affinity and interaction patterns of methyl gallate (a main constituent of ASSE) and chitosan against H. pylori urease (PDB ID: 6ZJA). Docking results revealed favorable binding scores for both ligands, with chitosan showing higher affinity (S score: − 6.42 kcal/mol) compared to methyl gallate (S score: − 5.45 kcal/mol). Key hydrogen bond interactions were observed with active site residues ASP362 and ALA169 for methyl gallate, and ASP223 and HIS323 for chitosan. These results suggest that ASSE@CS-NPs possess inhibitory potential against H. pylori and HCT116 cells. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Bioresources & Bioprocessing is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1186/s40643-026-01031-6
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