Academic Journal
Precision RNAi in Tomato Using Synthetic Trans‐Acting Small Interfering RNAs Derived From Minimal Precursors.
| Τίτλος: | Precision RNAi in Tomato Using Synthetic Trans‐Acting Small Interfering RNAs Derived From Minimal Precursors. |
|---|---|
| Συγγραφείς: | Tomassi, Ariel H., Juárez‐Molina, María, Cisneros, Adriana E., Alarcia, Ana, Orlando, Francesca, Toledano‐Franco, Sara, Presa, Silvia, Granell, Antonio, Carbonell, Alberto |
| Πηγή: | Plant Biotechnology Journal; Mar2026, Vol. 24 Issue 3, p1269-1284, 16p |
| Θεματικοί όροι: | Small interfering RNA, Tomatoes, Gene silencing, Crop improvement, RNA synthesis, RNA interference, Antiviral agents |
| Περίληψη: | RNA interference (RNAi) is a highly conserved gene silencing mechanism regulating gene expression at transcriptional and post‐transcriptional levels in plants. Synthetic trans‐acting small interfering RNAs (syn‐tasiRNAs) have emerged as powerful tools for highly specific and efficient gene silencing. However, their application in crops has been constrained by the need for transgene integration and the relatively long length of TAS‐derived precursors. Here, we developed a novel syn‐tasiRNA platform for Solanum lycopersicum (tomato) based on minimal precursors targeted by endogenous SlmiR482b microRNA. These minimal precursors, comprising only a 22‐nt miRNA target site, an 11‐nt spacer, and the syn‐tasiRNA sequence(s), effectively produced functional syn‐tasiRNAs in both transgenic and transient virus‐induced gene silencing (syn‐tasiR‐VIGS) systems. To facilitate their broader application, we engineered a series of vectors for high‐throughput cloning and efficient syn‐tasiRNA expression from SlmiR482b‐based minimal precursors in tomato. Our results show that minimal precursors induce robust gene silencing of endogenous tomato genes and confer antiviral resistance to the economically important tomato spotted wilt virus. Furthermore, we show that syn‐tasiR‐VIGS can be applied in a transgene‐free manner through crude extract delivery, leading to efficient silencing of endogenous genes. This study establishes minimal syn‐tasiRNA precursors as a versatile and efficient tool for precision RNAi in tomato, with applications in functional genomics and crop improvement. [ABSTRACT FROM AUTHOR] |
| Copyright of Plant Biotechnology Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Βάση Δεδομένων: | Complementary Index |
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| Header | DbId: edb DbLabel: Complementary Index An: 192088097 RelevancyScore: 1041 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1041.07177734375 |
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| Items | – Name: Title Label: Title Group: Ti Data: Precision RNAi in Tomato Using Synthetic Trans‐Acting Small Interfering RNAs Derived From Minimal Precursors. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Tomassi%2C+Ariel+H%2E%22">Tomassi, Ariel H.</searchLink><br /><searchLink fieldCode="AR" term="%22Juárez‐Molina%2C+María%22">Juárez‐Molina, María</searchLink><br /><searchLink fieldCode="AR" term="%22Cisneros%2C+Adriana+E%2E%22">Cisneros, Adriana E.</searchLink><br /><searchLink fieldCode="AR" term="%22Alarcia%2C+Ana%22">Alarcia, Ana</searchLink><br /><searchLink fieldCode="AR" term="%22Orlando%2C+Francesca%22">Orlando, Francesca</searchLink><br /><searchLink fieldCode="AR" term="%22Toledano‐Franco%2C+Sara%22">Toledano‐Franco, Sara</searchLink><br /><searchLink fieldCode="AR" term="%22Presa%2C+Silvia%22">Presa, Silvia</searchLink><br /><searchLink fieldCode="AR" term="%22Granell%2C+Antonio%22">Granell, Antonio</searchLink><br /><searchLink fieldCode="AR" term="%22Carbonell%2C+Alberto%22">Carbonell, Alberto</searchLink> – Name: TitleSource Label: Source Group: Src Data: Plant Biotechnology Journal; Mar2026, Vol. 24 Issue 3, p1269-1284, 16p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Small+interfering+RNA%22">Small interfering RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Tomatoes%22">Tomatoes</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+silencing%22">Gene silencing</searchLink><br /><searchLink fieldCode="DE" term="%22Crop+improvement%22">Crop improvement</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+synthesis%22">RNA synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22RNA+interference%22">RNA interference</searchLink><br /><searchLink fieldCode="DE" term="%22Antiviral+agents%22">Antiviral agents</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: RNA interference (RNAi) is a highly conserved gene silencing mechanism regulating gene expression at transcriptional and post‐transcriptional levels in plants. Synthetic trans‐acting small interfering RNAs (syn‐tasiRNAs) have emerged as powerful tools for highly specific and efficient gene silencing. However, their application in crops has been constrained by the need for transgene integration and the relatively long length of TAS‐derived precursors. Here, we developed a novel syn‐tasiRNA platform for Solanum lycopersicum (tomato) based on minimal precursors targeted by endogenous SlmiR482b microRNA. These minimal precursors, comprising only a 22‐nt miRNA target site, an 11‐nt spacer, and the syn‐tasiRNA sequence(s), effectively produced functional syn‐tasiRNAs in both transgenic and transient virus‐induced gene silencing (syn‐tasiR‐VIGS) systems. To facilitate their broader application, we engineered a series of vectors for high‐throughput cloning and efficient syn‐tasiRNA expression from SlmiR482b‐based minimal precursors in tomato. Our results show that minimal precursors induce robust gene silencing of endogenous tomato genes and confer antiviral resistance to the economically important tomato spotted wilt virus. Furthermore, we show that syn‐tasiR‐VIGS can be applied in a transgene‐free manner through crude extract delivery, leading to efficient silencing of endogenous genes. This study establishes minimal syn‐tasiRNA precursors as a versatile and efficient tool for precision RNAi in tomato, with applications in functional genomics and crop improvement. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Plant Biotechnology Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/pbi.70410 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 16 StartPage: 1269 Subjects: – SubjectFull: Small interfering RNA Type: general – SubjectFull: Tomatoes Type: general – SubjectFull: Gene silencing Type: general – SubjectFull: Crop improvement Type: general – SubjectFull: RNA synthesis Type: general – SubjectFull: RNA interference Type: general – SubjectFull: Antiviral agents Type: general Titles: – TitleFull: Precision RNAi in Tomato Using Synthetic Trans‐Acting Small Interfering RNAs Derived From Minimal Precursors. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Tomassi, Ariel H. – PersonEntity: Name: NameFull: Juárez‐Molina, María – PersonEntity: Name: NameFull: Cisneros, Adriana E. – PersonEntity: Name: NameFull: Alarcia, Ana – PersonEntity: Name: NameFull: Orlando, Francesca – PersonEntity: Name: NameFull: Toledano‐Franco, Sara – PersonEntity: Name: NameFull: Presa, Silvia – PersonEntity: Name: NameFull: Granell, Antonio – PersonEntity: Name: NameFull: Carbonell, Alberto IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 14677644 Numbering: – Type: volume Value: 24 – Type: issue Value: 3 Titles: – TitleFull: Plant Biotechnology Journal Type: main |
| ResultId | 1 |