Academic Journal
Extraction efficiency and activity determinants of soybean urease for sustainable biomineralization technology.
| Τίτλος: | Extraction efficiency and activity determinants of soybean urease for sustainable biomineralization technology. |
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| Συγγραφείς: | Chen, Yuyuan, Hazarika, Hemanta |
| Πηγή: | Smart Construction & Sustainable Cities; Dec2025, Vol. 3 Issue 1, p1-12, 12p |
| Θεματικοί όροι: | Biomineralization, Enzymes, Biocatalysis, Geotechnical engineering, Calcium carbonate, Enzymology |
| Περίληψη: | Sustainable biomineralization technologies rely on the efficient hydrolysis of urea, typically catalyzed by urease-producing microorganisms or purified enzymes. However, conventional approaches such as microbially induced calcium carbonate precipitation (MICP) and enzyme induced calcium carbonate precipitation (EICP) face limitations related to biosafety risks and high costs, respectively. In this study, soybean, an abundant and low-cost agricultural resource, was utilized to extract urease through a soaking and centrifugation process. A geometric modeling approach using tri-axial ellipsoid theory was introduced to explain how soybean grain size affects sieved powder yield. The effects of soybean grain size, powder concentration, temperature, pH, and storage conditions on the urease activity were systematically evaluated. Results showed that smaller soybean particle sizes resulted in lower extraction efficiency, whereas medium-grain soybeans provided the most cost-effective source due to their higher sieved powder yield and lower market price. Urease activity was positively correlated with both powder concentration and temperature within the tested range and reached its maximum at approximately pH 8. Additionally, storage at 4 °C significantly preserved the enzyme's initial activity over 72 h compared to room temperature conditions. These findings establish a practical foundation for the cost-effective production of plant-derived urease, promoting broader application of biomineralization techniques in sustainable geotechnical engineering. [ABSTRACT FROM AUTHOR] |
| Copyright of Smart Construction & Sustainable Cities is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Βάση Δεδομένων: | Complementary Index |
| FullText | Text: Availability: 0 |
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| Header | DbId: edb DbLabel: Complementary Index An: 191771690 RelevancyScore: 1041 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1040.81262207031 |
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| Items | – Name: Title Label: Title Group: Ti Data: Extraction efficiency and activity determinants of soybean urease for sustainable biomineralization technology. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Chen%2C+Yuyuan%22">Chen, Yuyuan</searchLink><br /><searchLink fieldCode="AR" term="%22Hazarika%2C+Hemanta%22">Hazarika, Hemanta</searchLink> – Name: TitleSource Label: Source Group: Src Data: Smart Construction & Sustainable Cities; Dec2025, Vol. 3 Issue 1, p1-12, 12p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Biomineralization%22">Biomineralization</searchLink><br /><searchLink fieldCode="DE" term="%22Enzymes%22">Enzymes</searchLink><br /><searchLink fieldCode="DE" term="%22Biocatalysis%22">Biocatalysis</searchLink><br /><searchLink fieldCode="DE" term="%22Geotechnical+engineering%22">Geotechnical engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Calcium+carbonate%22">Calcium carbonate</searchLink><br /><searchLink fieldCode="DE" term="%22Enzymology%22">Enzymology</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Sustainable biomineralization technologies rely on the efficient hydrolysis of urea, typically catalyzed by urease-producing microorganisms or purified enzymes. However, conventional approaches such as microbially induced calcium carbonate precipitation (MICP) and enzyme induced calcium carbonate precipitation (EICP) face limitations related to biosafety risks and high costs, respectively. In this study, soybean, an abundant and low-cost agricultural resource, was utilized to extract urease through a soaking and centrifugation process. A geometric modeling approach using tri-axial ellipsoid theory was introduced to explain how soybean grain size affects sieved powder yield. The effects of soybean grain size, powder concentration, temperature, pH, and storage conditions on the urease activity were systematically evaluated. Results showed that smaller soybean particle sizes resulted in lower extraction efficiency, whereas medium-grain soybeans provided the most cost-effective source due to their higher sieved powder yield and lower market price. Urease activity was positively correlated with both powder concentration and temperature within the tested range and reached its maximum at approximately pH 8. Additionally, storage at 4 °C significantly preserved the enzyme's initial activity over 72 h compared to room temperature conditions. These findings establish a practical foundation for the cost-effective production of plant-derived urease, promoting broader application of biomineralization techniques in sustainable geotechnical engineering. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Smart Construction & Sustainable Cities is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s44268-025-00056-8 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 1 Subjects: – SubjectFull: Biomineralization Type: general – SubjectFull: Enzymes Type: general – SubjectFull: Biocatalysis Type: general – SubjectFull: Geotechnical engineering Type: general – SubjectFull: Calcium carbonate Type: general – SubjectFull: Enzymology Type: general Titles: – TitleFull: Extraction efficiency and activity determinants of soybean urease for sustainable biomineralization technology. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Chen, Yuyuan – PersonEntity: Name: NameFull: Hazarika, Hemanta IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2025 Type: published Y: 2025 Numbering: – Type: volume Value: 3 – Type: issue Value: 1 Titles: – TitleFull: Smart Construction & Sustainable Cities Type: main |
| ResultId | 1 |