Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Smartphone-based colorimetric detection of urease activity in dental plaque for point-of-care screening of Helicobacter pylori infection. |
| Συγγραφείς: |
Wang, Yuhan, Zhang, Ping, Zhou, Chuxian, Zheng, Zicong, Quan, Qiang, He, Guangbo, Zhai, Hening, Song, Qifang, Zhang, Xiaoli, Li, Heng, Peng, Lanhuan, Liao, Yuqin, Fang, Wei, Li, Minmin, Liang, Jiajie, Tang, Yong |
| Πηγή: |
Microchimica Acta; Oct2025, Vol. 192 Issue 10, p1-10, 10p |
| Θεματικοί όροι: |
Helicobacter pylori, Point-of-care testing, Microsensors, Colorimetric analysis, Enzymology, Dental plaque, Smartphones, Public health |
| Περίληψη: |
Helicobacter pylori (H. pylori) is a major pathogen associated with gastric ulcers and gastric cancer, infecting approximately half of the global population. Therefore, developing sensitive, rapid, and cost-effective detection methods for H. pylori is critical for public health. We developed an innovative microscale colorimetric microsensor integrated with smartphone ambient-light sensors for portable, on-site quantification of H. pylori-associated urease activity in dental plaque. This low-cost device (< $ 5 fabrication) employs 3D-printed components and a custom app to measure transmittance changes from urease-catalyzed urea hydrolysis with phenol red, achieving a robust correlation with commercial microplate reader absorbance (R2 = 0.9989). The system demonstrates exceptional Analytical sensitivity, with limits of detection of 9.75 U/L for urease And 1.709 × 103 CFU/mL for H. pylori, alongside high reproducibility (CV < 10%) And stability over 14 days. When applied to the on-site detection of dental plaque among volunteers, the smartphone-based colorimetric microsensor exhibited detection results that were highly aligned with H. pylori infections confirmed by the urea breath test. It outperformed commercial test strips And achieved 100% sensitivity, 95.5% specificity, And 97.1% accuracy. This miniaturized, smartphone-based platform offers a promising solution for home self-testing and demonstrates significant potential for enhancing H. pylori preliminary screening at the community level. [ABSTRACT FROM AUTHOR] |
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| Βάση Δεδομένων: |
Complementary Index |