Academic Journal
The Effect of 96% Ethanol Extract of Semanggi leaves (Marsilea crenata Presl.) on Locomotor Activity and Alpha-synuclein Aggregation in Zebrafish (Danio rerio) Parkinson's Disease Model.
| Title: | The Effect of 96% Ethanol Extract of Semanggi leaves (Marsilea crenata Presl.) on Locomotor Activity and Alpha-synuclein Aggregation in Zebrafish (Danio rerio) Parkinson's Disease Model. |
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| Authors: | Muslikh, Faisal A., Ulhaq, Zulvikar S., Hardjono, Suko, Agil, Mangestuti |
| Source: | Tropical Journal of Natural Product Research; Sep2025, Vol. 9 Issue 9, p4143-4147, 5p |
| Subject Terms: | Parkinson's disease, Alpha-synuclein, Ethanol, Laboratory zebrafish, Ferns, Neuroprotective agents, Locomotor control |
| Abstract: | Parkinson's Disease (PD) is a neurodegenerative condition caused by the loss of dopaminergic neurons in the substantia nigra pars compacta (SNc) of the brain. Various factors such as aging, genetic predisposition, and exposure to environmental toxins like rotenone causes PD. Current PD treatments primarily manage motor symptoms, but long-term use of such therapy comes with side effects, necessitating the search for a potential drug with minimal adverse effects. Marsilea crenata Presl., commonly known as "Semanggi," is a plant recognized for its antineuroinflammatory activity. This study aimed to evaluate the potential neuroprotective effect of Marsilea crenata Presl leaf extract by assessing alpha-synuclein levels and locomotor activity in a zebrafish (Danio rerio) model of PD. Marsilea crenata Presl. leaves were extract by ultrasonicassisted extraction (UAE) method for 3 x 10 minutes. Zebrafish were induced with 5 ppb of rotenone and subsequently treated with 96% ethanol extract of Marsilea crenata Presl. leaves at various concentrations (2.5, 5, 10 and 20 ppm) for 28 days. Locomotor activity was observed weekly, while alpha-synuclein expression was measured using immunohistochemistry on day 28. The results indicate that 2.5 ppm of 96% ethanol leaf extract of Marsilea crenata Presl. was the optimal concentration that enhanced locomotor activity and reduced alpha-synuclein expression, with an average expression level of 42 ± 4-fold compared to 55 ± 6-fold in the rotenone group. Thus, 96% ethanol leaf extract of Marsilea crenata Presl. Exhibits antineurodegenerative activity, specifically in improving PD symptoms. Further study is required to confirm its molecular mechanism of action. [ABSTRACT FROM AUTHOR] |
| Copyright of Tropical Journal of Natural Product Research is the property of University of Benin, Faculty of Pharmacy and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Complementary Index |
| FullText | Text: Availability: 0 |
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| Header | DbId: edb DbLabel: Complementary Index An: 188512535 RelevancyScore: 1023 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1023.08752441406 |
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| Items | – Name: Title Label: Title Group: Ti Data: The Effect of 96% Ethanol Extract of Semanggi leaves (Marsilea crenata Presl.) on Locomotor Activity and Alpha-synuclein Aggregation in Zebrafish (Danio rerio) Parkinson's Disease Model. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Muslikh%2C+Faisal+A%2E%22">Muslikh, Faisal A.</searchLink><br /><searchLink fieldCode="AR" term="%22Ulhaq%2C+Zulvikar+S%2E%22">Ulhaq, Zulvikar S.</searchLink><br /><searchLink fieldCode="AR" term="%22Hardjono%2C+Suko%22">Hardjono, Suko</searchLink><br /><searchLink fieldCode="AR" term="%22Agil%2C+Mangestuti%22">Agil, Mangestuti</searchLink> – Name: TitleSource Label: Source Group: Src Data: Tropical Journal of Natural Product Research; Sep2025, Vol. 9 Issue 9, p4143-4147, 5p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Parkinson's+disease%22">Parkinson's disease</searchLink><br /><searchLink fieldCode="DE" term="%22Alpha-synuclein%22">Alpha-synuclein</searchLink><br /><searchLink fieldCode="DE" term="%22Ethanol%22">Ethanol</searchLink><br /><searchLink fieldCode="DE" term="%22Laboratory+zebrafish%22">Laboratory zebrafish</searchLink><br /><searchLink fieldCode="DE" term="%22Ferns%22">Ferns</searchLink><br /><searchLink fieldCode="DE" term="%22Neuroprotective+agents%22">Neuroprotective agents</searchLink><br /><searchLink fieldCode="DE" term="%22Locomotor+control%22">Locomotor control</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Parkinson's Disease (PD) is a neurodegenerative condition caused by the loss of dopaminergic neurons in the substantia nigra pars compacta (SNc) of the brain. Various factors such as aging, genetic predisposition, and exposure to environmental toxins like rotenone causes PD. Current PD treatments primarily manage motor symptoms, but long-term use of such therapy comes with side effects, necessitating the search for a potential drug with minimal adverse effects. Marsilea crenata Presl., commonly known as "Semanggi," is a plant recognized for its antineuroinflammatory activity. This study aimed to evaluate the potential neuroprotective effect of Marsilea crenata Presl leaf extract by assessing alpha-synuclein levels and locomotor activity in a zebrafish (Danio rerio) model of PD. Marsilea crenata Presl. leaves were extract by ultrasonicassisted extraction (UAE) method for 3 x 10 minutes. Zebrafish were induced with 5 ppb of rotenone and subsequently treated with 96% ethanol extract of Marsilea crenata Presl. leaves at various concentrations (2.5, 5, 10 and 20 ppm) for 28 days. Locomotor activity was observed weekly, while alpha-synuclein expression was measured using immunohistochemistry on day 28. The results indicate that 2.5 ppm of 96% ethanol leaf extract of Marsilea crenata Presl. was the optimal concentration that enhanced locomotor activity and reduced alpha-synuclein expression, with an average expression level of 42 ± 4-fold compared to 55 ± 6-fold in the rotenone group. Thus, 96% ethanol leaf extract of Marsilea crenata Presl. Exhibits antineurodegenerative activity, specifically in improving PD symptoms. Further study is required to confirm its molecular mechanism of action. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Tropical Journal of Natural Product Research is the property of University of Benin, Faculty of Pharmacy and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.26538/tjnpr/v9i9.10 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 5 StartPage: 4143 Subjects: – SubjectFull: Parkinson's disease Type: general – SubjectFull: Alpha-synuclein Type: general – SubjectFull: Ethanol Type: general – SubjectFull: Laboratory zebrafish Type: general – SubjectFull: Ferns Type: general – SubjectFull: Neuroprotective agents Type: general – SubjectFull: Locomotor control Type: general Titles: – TitleFull: The Effect of 96% Ethanol Extract of Semanggi leaves (Marsilea crenata Presl.) on Locomotor Activity and Alpha-synuclein Aggregation in Zebrafish (Danio rerio) Parkinson's Disease Model. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Muslikh, Faisal A. – PersonEntity: Name: NameFull: Ulhaq, Zulvikar S. – PersonEntity: Name: NameFull: Hardjono, Suko – PersonEntity: Name: NameFull: Agil, Mangestuti IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2025 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 26160684 Numbering: – Type: volume Value: 9 – Type: issue Value: 9 Titles: – TitleFull: Tropical Journal of Natural Product Research Type: main |
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