Academic Journal
Encapsulation of β-Galactosidase into Polyallylamine/Polystyrene Sulphonate Polyelectrolyte Microcapsules.
| Title: | Encapsulation of β-Galactosidase into Polyallylamine/Polystyrene Sulphonate Polyelectrolyte Microcapsules. |
|---|---|
| Authors: | Chebykin, Yuri S., Musin, Egor V., Kim, Aleksandr L., Tikhonenko, Sergey A. |
| Source: | International Journal of Molecular Sciences; Oct2024, Vol. 25 Issue 20, p10978, 12p |
| Subject Terms: | Enzyme replacement therapy, Lactose intolerance, Ionic strength, Coprecipitation (Chemistry), Dairy products |
| Abstract: | More than half of the global population is unable to consume dairy products due to lactose intolerance (hypolactasia). Current enzyme replacement therapy methods are insufficiently effective as a therapeutic approach to treating lactose intolerance. The encapsulation of β-galactosidase in polyelectrolyte microcapsules by using the layer-by-layer method could be a possible solution to this problem. In this study, adsorption and co-precipitation methods were employed for encapsulating β-galactosidase in polyelectrolyte microcapsules composed of (polyallylamine /polystyrene sulphonate)₃. As a result, the co-precipitation method was chosen for β-galactosidase encapsulation. The adsorption method permits to encapsulate six times less enzyme compared with the co-precipitation method; the β-galactosidase encapsulated via the co-precipitation method released no more than 20% of the initially encapsulated enzyme in pH 2 or 1 M NaCl solutions. In contrast, when using the sorption method, about 100% of the initially encapsulated enzyme was released from the microcapsules under the conditions described above. The co-precipitation method effectively prevents the complete loss of enzyme activity after 2 h of incubation in a solution with pH 2 while also alleviating the adverse effects of ionic strength. Consequently, the encapsulated form of β-galactosidase shows promise as a potential therapeutic agent for enzyme replacement therapy in the treatment of hypolactasia. [ABSTRACT FROM AUTHOR] |
| Copyright of International Journal of Molecular Sciences is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Complementary Index |
| FullText | Text: Availability: 0 CustomLinks: – Url: https://resolver.ebsco.com/c/fiv2js/result?sid=EBSCO:edb&genre=article&issn=16616596&ISBN=&volume=25&issue=20&date=20241015&spage=10978&pages=10978-10989&title=International Journal of Molecular Sciences&atitle=Encapsulation%20of%20%CE%B2-Galactosidase%20into%20Polyallylamine%2FPolystyrene%20Sulphonate%20Polyelectrolyte%20Microcapsules.&aulast=Chebykin%2C%20Yuri%20S.&id=DOI:10.3390/ijms252010978 Name: Full Text Finder (for New FTF UI) (ns324271) Category: fullText Text: Full Text Finder MouseOverText: Full Text Finder |
|---|---|
| Header | DbId: edb DbLabel: Complementary Index An: 180487207 RelevancyScore: 974 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 973.593383789063 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Encapsulation of β-Galactosidase into Polyallylamine/Polystyrene Sulphonate Polyelectrolyte Microcapsules. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Chebykin%2C+Yuri+S%2E%22">Chebykin, Yuri S.</searchLink><br /><searchLink fieldCode="AR" term="%22Musin%2C+Egor+V%2E%22">Musin, Egor V.</searchLink><br /><searchLink fieldCode="AR" term="%22Kim%2C+Aleksandr+L%2E%22">Kim, Aleksandr L.</searchLink><br /><searchLink fieldCode="AR" term="%22Tikhonenko%2C+Sergey+A%2E%22">Tikhonenko, Sergey A.</searchLink> – Name: TitleSource Label: Source Group: Src Data: International Journal of Molecular Sciences; Oct2024, Vol. 25 Issue 20, p10978, 12p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Enzyme+replacement+therapy%22">Enzyme replacement therapy</searchLink><br /><searchLink fieldCode="DE" term="%22Lactose+intolerance%22">Lactose intolerance</searchLink><br /><searchLink fieldCode="DE" term="%22Ionic+strength%22">Ionic strength</searchLink><br /><searchLink fieldCode="DE" term="%22Coprecipitation+%28Chemistry%29%22">Coprecipitation (Chemistry)</searchLink><br /><searchLink fieldCode="DE" term="%22Dairy+products%22">Dairy products</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: More than half of the global population is unable to consume dairy products due to lactose intolerance (hypolactasia). Current enzyme replacement therapy methods are insufficiently effective as a therapeutic approach to treating lactose intolerance. The encapsulation of β-galactosidase in polyelectrolyte microcapsules by using the layer-by-layer method could be a possible solution to this problem. In this study, adsorption and co-precipitation methods were employed for encapsulating β-galactosidase in polyelectrolyte microcapsules composed of (polyallylamine /polystyrene sulphonate)₃. As a result, the co-precipitation method was chosen for β-galactosidase encapsulation. The adsorption method permits to encapsulate six times less enzyme compared with the co-precipitation method; the β-galactosidase encapsulated via the co-precipitation method released no more than 20% of the initially encapsulated enzyme in pH 2 or 1 M NaCl solutions. In contrast, when using the sorption method, about 100% of the initially encapsulated enzyme was released from the microcapsules under the conditions described above. The co-precipitation method effectively prevents the complete loss of enzyme activity after 2 h of incubation in a solution with pH 2 while also alleviating the adverse effects of ionic strength. Consequently, the encapsulated form of β-galactosidase shows promise as a potential therapeutic agent for enzyme replacement therapy in the treatment of hypolactasia. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of International Journal of Molecular Sciences is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=180487207 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/ijms252010978 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 10978 Subjects: – SubjectFull: Enzyme replacement therapy Type: general – SubjectFull: Lactose intolerance Type: general – SubjectFull: Ionic strength Type: general – SubjectFull: Coprecipitation (Chemistry) Type: general – SubjectFull: Dairy products Type: general Titles: – TitleFull: Encapsulation of β-Galactosidase into Polyallylamine/Polystyrene Sulphonate Polyelectrolyte Microcapsules. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Chebykin, Yuri S. – PersonEntity: Name: NameFull: Musin, Egor V. – PersonEntity: Name: NameFull: Kim, Aleksandr L. – PersonEntity: Name: NameFull: Tikhonenko, Sergey A. IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 10 Text: Oct2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 16616596 Numbering: – Type: volume Value: 25 – Type: issue Value: 20 Titles: – TitleFull: International Journal of Molecular Sciences Type: main |
| ResultId | 1 |