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长链非编码 RNA 1010001N08Rik 和 GATA6 在 高氧暴露小鼠肺癌细胞中表达和意义.

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Τίτλος: 长链非编码 RNA 1010001N08Rik 和 GATA6 在 高氧暴露小鼠肺癌细胞中表达和意义. (Chinese)
Alternate Title: Expression and Significance of Long non-coding RNA 1010001N08Rik and GATA 6 in Hyperoxia-exposed LLC Cells. (English)
Συγγραφείς: 罗 好, 刘怡波, 颜崇兵, 翁博雯, 蔡 成
Πηγή: Progress in Modern Biomedicine; 2024, Vol. 24 Issue 15, p2805-2816, 12p
Θεματικοί όροι: Gene expression, RNA interference, Small interfering RNA, LincRNA, Wnt proteins
Abstract (English): Objective: To investigate the expression changes and correlation of long non-coding RNA (lncRNA) 1010001N08Rik and GATA6 in hyperoxic LLC cells, and the mechanism of Wnt/β-catenin signaling pathway involved in hyperoxic lung injury. Method: (1) LLC cells were divided into air group. The expression levels of GATA6, Wnt, β-catenin, 1010001N08Rik in each group were detected by RT-PCR and the expression levels of related proteins in each group were detected by Western blot. (2) LLC cells were divided into non-interference air group, non-interference hyperoxia group, NC-si RNA interference hyperoxia group and si RNA interference hyperoxia group. The expression levels of GATA6, Wnt, β-catenin, 1010001N08Rik in each group were detected by RT-PCR and the expression levels of related proteins in each group were detected by Western blot. The apoptosis was detected by flow cytometry. (3) Data visualization using GraphPad Prism 9.4.1. (4) SPSS 26.0 statistical software was used for data processing. The data of two groups were compared by t test. One-way analysis of variance was used for comparison among multiple groups. (5) Wnt, Fzd2, GATA6 and other related genes were input into string11.0 database to construct the PPI network and derive the PPI network diagram. Results: (1) Compared with the air group, the expression of GATA6 m RNA decreased in the hyperoxia exposure group for 24 h and hyperoxia exposure group for 48h, the expression of lncRNA1010001N08Rik m RNA, Wnt m RNA, β-catenin m RNA expression increased. The difference was statistically significant (P＜0.05) . (2) Compared with the air group, the expression of GATA6 protein decreased in the hyperoxia exposure group for 24 h and hyperoxia exposure group for 48 h, whlie the expression of Wnt protein, β-catenin protein increased. The difference was statistically significant (P＜0.05) . (3) Compared with the non-interference air group, the expression of GATA6 m RNA and protein in the non-interference hyperoxia group and the NC-si RNA interference hyperoxia group decreased, and the expression of 1010001N08Rik m RNA, Wnt m RNA and protein, β-catenin m RNA and protein increased. There was no significant difference between non-interference hyperoxia group and NC-si RNA interference hyperoxia group. Compared with the non-interference hyperoxia group and the NC-si RNA interference hyperoxia group, the expression of GATA6 m RNA and protein in the si RNA interference hyperoxia group increased, and the expression of 1010001N08Rik m RNA, Wnt m RNA and protein, β-catenin m RNA and protein in the si RNA interference hyperoxia group decreased. The difference was statistically significant (P＜0.05) . (4) Compared with the non-interference air group, the apoptosis rate of the non-interference hyperoxia group and the NC-si RNA interfered hyperoxia group increased. There was no significant difference between non-interference hyperoxia group and NC-si RNA interference hyperoxia group. Compared with the non-interference hyperoxia group and the NC-si RNA interference hyperoxia group, the apoptosis rate of si RNA interference hyperoxia group decreased. The differences were statistically significant (P＜0.05). (5) According to the protein-protein interaction (PPI) network diagram of Wnt, Fzd2, GATA6 and other related genes derived from the string11.0 database, it can be seen that GATA6 is closely related to Wnt. Conclusion: In LCC cells exposed to hyperoxia, 1010001N08Rik may activate Wnt/β-catenin signaling pathway by down-regulating the expression of GATA6, thus promoting the formation process of hyperoxic lung injury. [ABSTRACT FROM AUTHOR]
Abstract (Chinese): 目的: 探讨长链非编码 RNA（long noncoding RNA, lncRNA) 1010001N08Rik 和 GATA6 在高氧暴露小鼠肺癌细胞（Lewis lung carcinoma cell, LLC 细胞) 中表达量的变化与相关性, 及其介导 Wnt/茁-catenin 信号通路参与高氧肺损伤的机制. 方法: (1) 将 LLC 细胞分为空气组, 高氧暴露 24 h 组和高氧暴露 48h 组, 应用 RT-PCR 和 Western blot 法分别检测 lncRNA1010001N08Rik, GATA6, Wnt 及 茁-catenin 的 mRNA 及相关蛋白表达水平. (2) 将 LLC 细胞分为无干扰空气组, 无干扰高氧组, NC-siRNA 干扰高 氧组, siRNA 干扰高氧组. 应用 RT-PCR 和 Western blot 法分别检测 lncRNA1010001N08Rik, GATA6, Wnt 及 茁-catenin 的 mRNA 及相关蛋白表达水平, 流式细胞术检测各组细胞凋亡. (3) 利用 GraphPad Prism 9.4.1 进行数据可视化. (4) 采用 SPSS 26.0 统计软 件进行数据处理, 两组间数据比较采用两独立样本 t 检验, 多组间比较采用单因素方差分析. (5) 将 Wnt, Fzd2, GATA6 等相关基 因输入 string11.0 数据库构建 PPI 网络并导出 PPI 网络图. 结果: (1) 与空气组相比较, 高氧暴露 24 h 组和 48h 组 GATA6 的 mRNA 表达量下降, lncRNA1010001N08Rik, Wnt, 茁-catenin 的 mRNA 表达量增加, 差异具有统计学意义 (P＜0.05) . (2) 与空气组 相比, 高氧暴露 24 h 组和 48 h 组 GATA6 蛋白表达量下降, Wnt, 茁-catenin 蛋白表达量增加, 差异具有统计学意义（P＜0.05) . (3) 与无干扰空气组相比, 无干扰高氧组, NC-siRNA 干扰高氧组的 GATA6 的 mRNA 表达量和蛋白表达量下降, 1010001N08Rik 的 mRNA 表达量, Wnt, 茁-catenin 的 mRNA 表达量和蛋白表达量增加; 无干扰高氧组与 NC-siRNA 干扰高氧组的比较差异无统计学 意义; 与干扰高氧组与 NC-siRNA 干扰高氧组相比, siRNA 干扰高氧组 GATA6 的 mRNA 表达量和蛋白表达量增加, 1010001N08Rik 的 mRNA 表达量, Wnt, 茁-catenin 的 mRNA 表达量和蛋白表达量下降, 差异均具有统计学意义（P<0.05) . (4) 与无 干扰空气组相比, 无干扰高氧组, NC-siRNA 干扰高氧组细胞凋亡率增加; 无干扰高氧组与 NC-siRNA 干扰高氧组的比较差异无 统计学意义；与无干扰高氧组与 NC-siRNA 干扰高氧组相比, siRNA 干扰高氧组的细胞凋亡率下降, 差异均具有统计学意义 (P<0. 05) . (5) 根据 string11.0 数据库导出 Wnt, Fzd2, GATA6 等相关基因的 PPI 网络图显示, GATA6 与 Wnt 有密切相关. 结论：高氧暴 露下, LCC 细胞中 lncRNA1010001N08Rik 可能通过下调 GATA6 的表达激活 Wnt/茁-catenin 信号通路的传导, 从而促进高氧肺损 伤的形成过程. [ABSTRACT FROM AUTHOR]
Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: 长链非编码 RNA 1010001N08Rik 和 GATA6 在 高氧暴露小鼠肺癌细胞中表达和意义. (Chinese)
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  Data: Expression and Significance of Long non-coding RNA 1010001N08Rik and GATA 6 in Hyperoxia-exposed LLC Cells. (English)
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  Data: Progress in Modern Biomedicine; 2024, Vol. 24 Issue 15, p2805-2816, 12p
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  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Gene+expression%22&quot;&gt;Gene expression&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22RNA+interference%22&quot;&gt;RNA interference&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Small+interfering+RNA%22&quot;&gt;Small interfering RNA&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22LincRNA%22&quot;&gt;LincRNA&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Wnt+proteins%22&quot;&gt;Wnt proteins&lt;/searchLink&gt;
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  Data: Objective: To investigate the expression changes and correlation of long non-coding RNA (lncRNA) 1010001N08Rik and GATA6 in hyperoxic LLC cells, and the mechanism of Wnt/β-catenin signaling pathway involved in hyperoxic lung injury. Method: (1) LLC cells were divided into air group. The expression levels of GATA6, Wnt, β-catenin, 1010001N08Rik in each group were detected by RT-PCR and the expression levels of related proteins in each group were detected by Western blot. (2) LLC cells were divided into non-interference air group, non-interference hyperoxia group, NC-si RNA interference hyperoxia group and si RNA interference hyperoxia group. The expression levels of GATA6, Wnt, β-catenin, 1010001N08Rik in each group were detected by RT-PCR and the expression levels of related proteins in each group were detected by Western blot. The apoptosis was detected by flow cytometry. (3) Data visualization using GraphPad Prism 9.4.1. (4) SPSS 26.0 statistical software was used for data processing. The data of two groups were compared by t test. One-way analysis of variance was used for comparison among multiple groups. (5) Wnt, Fzd2, GATA6 and other related genes were input into string11.0 database to construct the PPI network and derive the PPI network diagram. Results: (1) Compared with the air group, the expression of GATA6 m RNA decreased in the hyperoxia exposure group for 24 h and hyperoxia exposure group for 48h, the expression of lncRNA1010001N08Rik m RNA, Wnt m RNA, β-catenin m RNA expression increased. The difference was statistically significant (P＜0.05) . (2) Compared with the air group, the expression of GATA6 protein decreased in the hyperoxia exposure group for 24 h and hyperoxia exposure group for 48 h, whlie the expression of Wnt protein, β-catenin protein increased. The difference was statistically significant (P＜0.05) . (3) Compared with the non-interference air group, the expression of GATA6 m RNA and protein in the non-interference hyperoxia group and the NC-si RNA interference hyperoxia group decreased, and the expression of 1010001N08Rik m RNA, Wnt m RNA and protein, β-catenin m RNA and protein increased. There was no significant difference between non-interference hyperoxia group and NC-si RNA interference hyperoxia group. Compared with the non-interference hyperoxia group and the NC-si RNA interference hyperoxia group, the expression of GATA6 m RNA and protein in the si RNA interference hyperoxia group increased, and the expression of 1010001N08Rik m RNA, Wnt m RNA and protein, β-catenin m RNA and protein in the si RNA interference hyperoxia group decreased. The difference was statistically significant (P＜0.05) . (4) Compared with the non-interference air group, the apoptosis rate of the non-interference hyperoxia group and the NC-si RNA interfered hyperoxia group increased. There was no significant difference between non-interference hyperoxia group and NC-si RNA interference hyperoxia group. Compared with the non-interference hyperoxia group and the NC-si RNA interference hyperoxia group, the apoptosis rate of si RNA interference hyperoxia group decreased. The differences were statistically significant (P＜0.05). (5) According to the protein-protein interaction (PPI) network diagram of Wnt, Fzd2, GATA6 and other related genes derived from the string11.0 database, it can be seen that GATA6 is closely related to Wnt. Conclusion: In LCC cells exposed to hyperoxia, 1010001N08Rik may activate Wnt/β-catenin signaling pathway by down-regulating the expression of GATA6, thus promoting the formation process of hyperoxic lung injury. [ABSTRACT FROM AUTHOR]
– Name: AbstractNonEng
  Label: Abstract (Chinese)
  Group: Ab
  Data: 目的: 探讨长链非编码 RNA（long noncoding RNA, lncRNA) 1010001N08Rik 和 GATA6 在高氧暴露小鼠肺癌细胞（Lewis lung carcinoma cell, LLC 细胞) 中表达量的变化与相关性, 及其介导 Wnt/茁-catenin 信号通路参与高氧肺损伤的机制. 方法: (1) 将 LLC 细胞分为空气组, 高氧暴露 24 h 组和高氧暴露 48h 组, 应用 RT-PCR 和 Western blot 法分别检测 lncRNA1010001N08Rik, GATA6, Wnt 及 茁-catenin 的 mRNA 及相关蛋白表达水平. (2) 将 LLC 细胞分为无干扰空气组, 无干扰高氧组, NC-siRNA 干扰高 氧组, siRNA 干扰高氧组. 应用 RT-PCR 和 Western blot 法分别检测 lncRNA1010001N08Rik, GATA6, Wnt 及 茁-catenin 的 mRNA 及相关蛋白表达水平, 流式细胞术检测各组细胞凋亡. (3) 利用 GraphPad Prism 9.4.1 进行数据可视化. (4) 采用 SPSS 26.0 统计软 件进行数据处理, 两组间数据比较采用两独立样本 t 检验, 多组间比较采用单因素方差分析. (5) 将 Wnt, Fzd2, GATA6 等相关基 因输入 string11.0 数据库构建 PPI 网络并导出 PPI 网络图. 结果: (1) 与空气组相比较, 高氧暴露 24 h 组和 48h 组 GATA6 的 mRNA 表达量下降, lncRNA1010001N08Rik, Wnt, 茁-catenin 的 mRNA 表达量增加, 差异具有统计学意义 (P＜0.05) . (2) 与空气组 相比, 高氧暴露 24 h 组和 48 h 组 GATA6 蛋白表达量下降, Wnt, 茁-catenin 蛋白表达量增加, 差异具有统计学意义（P＜0.05) . (3) 与无干扰空气组相比, 无干扰高氧组, NC-siRNA 干扰高氧组的 GATA6 的 mRNA 表达量和蛋白表达量下降, 1010001N08Rik 的 mRNA 表达量, Wnt, 茁-catenin 的 mRNA 表达量和蛋白表达量增加; 无干扰高氧组与 NC-siRNA 干扰高氧组的比较差异无统计学 意义; 与干扰高氧组与 NC-siRNA 干扰高氧组相比, siRNA 干扰高氧组 GATA6 的 mRNA 表达量和蛋白表达量增加, 1010001N08Rik 的 mRNA 表达量, Wnt, 茁-catenin 的 mRNA 表达量和蛋白表达量下降, 差异均具有统计学意义（P&lt;0.05) . (4) 与无 干扰空气组相比, 无干扰高氧组, NC-siRNA 干扰高氧组细胞凋亡率增加; 无干扰高氧组与 NC-siRNA 干扰高氧组的比较差异无 统计学意义；与无干扰高氧组与 NC-siRNA 干扰高氧组相比, siRNA 干扰高氧组的细胞凋亡率下降, 差异均具有统计学意义 (P&lt;0. 05) . (5) 根据 string11.0 数据库导出 Wnt, Fzd2, GATA6 等相关基因的 PPI 网络图显示, GATA6 与 Wnt 有密切相关. 结论：高氧暴 露下, LCC 细胞中 lncRNA1010001N08Rik 可能通过下调 GATA6 的表达激活 Wnt/茁-catenin 信号通路的传导, 从而促进高氧肺损 伤的形成过程. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: &lt;i&gt;Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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        Value: 10.13241/j.cnki.pmb.2024.15.002
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      – Code: chi
        Text: Chinese
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        PageCount: 12
        StartPage: 2805
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      – SubjectFull: Gene expression
        Type: general
      – SubjectFull: RNA interference
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      – SubjectFull: Small interfering RNA
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      – SubjectFull: Wnt proteins
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      – TitleFull: 长链非编码 RNA 1010001N08Rik 和 GATA6 在 高氧暴露小鼠肺癌细胞中表达和意义.
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              M: 08
              Text: 2024
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