Academic Journal
Design Rules for the Nano‐Bio Interface of Nanodiamonds: Implications for siRNA Vectorization.
| Τίτλος: | Design Rules for the Nano‐Bio Interface of Nanodiamonds: Implications for siRNA Vectorization. |
|---|---|
| Συγγραφείς: | Kindermann, Marek, Neburkova, Jitka, Neuhoferova, Eva, Majer, Jan, Stejfova, Miroslava, Benson, Veronika, Cigler, Petr |
| Πηγή: | Advanced Functional Materials; 7/24/2024, Vol. 34 Issue 30, p1-15, 15p |
| Θεματικοί όροι: | Small interfering RNA, Nanodiamonds, RNA, Colloidal stability, Protective coatings, Acrylamide |
| Περίληψη: | The enormous therapeutic potential of selective ribonucleic acid (RNA) interference has recently been manifested by the approval of several small interfering RNA (siRNA)‐based drugs. However, the efficacy of siRNA delivery is still limited, and an extensive search for alternative and highly effective delivery approaches is ongoing. With this aim, three generations of non‐viral vectors based on modified nanodiamonds (NDs) have been gradually developed in the past decade. They show great promise due to the negligible toxicity of the ND core. Here, a robust methodological approach is presented to enable the evaluation of new vector nanomaterials. Using a new type of third‐generation ND vector coated with a copolymer with tunable charge density, variables such as colloidal stability, surface electrostatic properties, the molecular composition of the copolymer, and the mode of complexation with siRNA are optimized. Using an innovative data processing strategy, the results are related to biological potency, toxicity, and cell proliferation. Finally, the optimized composition of a coating copolymer consisting of a cationic component, 2‐dimethylaminoethyl methacrylate, and an electroneutral biocompatible component, N‐(2‐hydroxypropyl) methacrylamide, is evaluated. The optimized NDs vectors are colloidally and biologically stable siRNA delivery tools with broad potential for RNA interference‐based therapeutics. [ABSTRACT FROM AUTHOR] |
| Copyright of Advanced Functional Materials is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Βάση Δεδομένων: | Complementary Index |
| FullText | Links: – Type: other Text: Availability: 0 |
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| Header | DbId: edb DbLabel: Complementary Index An: 178592334 RelevancyScore: 958 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 957.837036132813 |
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| Items | – Name: Title Label: Title Group: Ti Data: Design Rules for the Nano‐Bio Interface of Nanodiamonds: Implications for siRNA Vectorization. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kindermann%2C+Marek%22">Kindermann, Marek</searchLink><br /><searchLink fieldCode="AR" term="%22Neburkova%2C+Jitka%22">Neburkova, Jitka</searchLink><br /><searchLink fieldCode="AR" term="%22Neuhoferova%2C+Eva%22">Neuhoferova, Eva</searchLink><br /><searchLink fieldCode="AR" term="%22Majer%2C+Jan%22">Majer, Jan</searchLink><br /><searchLink fieldCode="AR" term="%22Stejfova%2C+Miroslava%22">Stejfova, Miroslava</searchLink><br /><searchLink fieldCode="AR" term="%22Benson%2C+Veronika%22">Benson, Veronika</searchLink><br /><searchLink fieldCode="AR" term="%22Cigler%2C+Petr%22">Cigler, Petr</searchLink> – Name: TitleSource Label: Source Group: Src Data: Advanced Functional Materials; 7/24/2024, Vol. 34 Issue 30, p1-15, 15p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Small+interfering+RNA%22">Small interfering RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Nanodiamonds%22">Nanodiamonds</searchLink><br /><searchLink fieldCode="DE" term="%22RNA%22">RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Colloidal+stability%22">Colloidal stability</searchLink><br /><searchLink fieldCode="DE" term="%22Protective+coatings%22">Protective coatings</searchLink><br /><searchLink fieldCode="DE" term="%22Acrylamide%22">Acrylamide</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The enormous therapeutic potential of selective ribonucleic acid (RNA) interference has recently been manifested by the approval of several small interfering RNA (siRNA)‐based drugs. However, the efficacy of siRNA delivery is still limited, and an extensive search for alternative and highly effective delivery approaches is ongoing. With this aim, three generations of non‐viral vectors based on modified nanodiamonds (NDs) have been gradually developed in the past decade. They show great promise due to the negligible toxicity of the ND core. Here, a robust methodological approach is presented to enable the evaluation of new vector nanomaterials. Using a new type of third‐generation ND vector coated with a copolymer with tunable charge density, variables such as colloidal stability, surface electrostatic properties, the molecular composition of the copolymer, and the mode of complexation with siRNA are optimized. Using an innovative data processing strategy, the results are related to biological potency, toxicity, and cell proliferation. Finally, the optimized composition of a coating copolymer consisting of a cationic component, 2‐dimethylaminoethyl methacrylate, and an electroneutral biocompatible component, N‐(2‐hydroxypropyl) methacrylamide, is evaluated. The optimized NDs vectors are colloidally and biologically stable siRNA delivery tools with broad potential for RNA interference‐based therapeutics. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Advanced Functional Materials is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/adfm.202314088 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 15 StartPage: 1 Subjects: – SubjectFull: Small interfering RNA Type: general – SubjectFull: Nanodiamonds Type: general – SubjectFull: RNA Type: general – SubjectFull: Colloidal stability Type: general – SubjectFull: Protective coatings Type: general – SubjectFull: Acrylamide Type: general Titles: – TitleFull: Design Rules for the Nano‐Bio Interface of Nanodiamonds: Implications for siRNA Vectorization. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kindermann, Marek – PersonEntity: Name: NameFull: Neburkova, Jitka – PersonEntity: Name: NameFull: Neuhoferova, Eva – PersonEntity: Name: NameFull: Majer, Jan – PersonEntity: Name: NameFull: Stejfova, Miroslava – PersonEntity: Name: NameFull: Benson, Veronika – PersonEntity: Name: NameFull: Cigler, Petr IsPartOfRelationships: – BibEntity: Dates: – D: 24 M: 07 Text: 7/24/2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 1616301X Numbering: – Type: volume Value: 34 – Type: issue Value: 30 Titles: – TitleFull: Advanced Functional Materials Type: main |
| ResultId | 1 |