A combined surface plasmonic and isotope-selective spectroscopic study toward a deeper understanding of real-time enzymatic urea hydrolysis.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	A combined surface plasmonic and isotope-selective spectroscopic study toward a deeper understanding of real-time enzymatic urea hydrolysis.
Συγγραφείς:	Banerjee, Jayeta, Pradhan, Manik
Πηγή:	Journal of Chemical Sciences; Jun2023, Vol. 135 Issue 2, p1-9, 9p
Περίληψη:	We employed the wavelength-interrogated surface plasmon resonance (SPR) method to characterize the real-time kinetics of urea-urease hydrolysis reaction in response to a CO2-free N2 environment and CO2-enriched ambient reaction medium. We established that a simple label-free SPR probe could accurately extract kinetic parameters from the nature of the sharp jump of the SPR wavelength shift in the reaction profile. The kinetic analysis showed that CO2 production increases with increasing reaction time irrespective of CO2-free N2 or CO2-enriched reaction environment. We also explored the essential insights into the isotopic fractionations of ¹²CO2, ¹³CO2, ¹²C¹⁸O¹⁶O in the reaction medium utilizing integrated cavity output spectroscopy. The plasmonic system measured the reaction rate in the order of 10^-7 M/s for urea species in the presence of the urease enzyme. This study deepens our understanding of plasmonic-based enzymatic urea hydrolysis in real time and opens a new way to quantify chemical reaction kinetics for various other systems. This is the first detailed experimental investigation of the real-time kinetics of urea-urease hydrolysis reaction exploiting wavelength-interrogated surface plasmon resonance method in response to produced CO2 in the CO2-free N2 environment and CO2-enriched ambient reaction medium utilizing integrated cavity output spectroscopy. [ABSTRACT FROM AUTHOR]
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Βάση Δεδομένων:	Complementary Index

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