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A combined surface plasmonic and isotope-selective spectroscopic study toward a deeper understanding of real-time enzymatic urea hydrolysis.

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Τίτλος: A combined surface plasmonic and isotope-selective spectroscopic study toward a deeper understanding of real-time enzymatic urea hydrolysis.
Συγγραφείς: Banerjee, Jayeta, Pradhan, Manik
Πηγή: Journal of Chemical Sciences; Jun2023, Vol. 135 Issue 2, p1-9, 9p
Περίληψη: We employed the wavelength-interrogated surface plasmon resonance (SPR) method to characterize the real-time kinetics of urea-urease hydrolysis reaction in response to a CO2-free N2 environment and CO2-enriched ambient reaction medium. We established that a simple label-free SPR probe could accurately extract kinetic parameters from the nature of the sharp jump of the SPR wavelength shift in the reaction profile. The kinetic analysis showed that CO2 production increases with increasing reaction time irrespective of CO2-free N2 or CO2-enriched reaction environment. We also explored the essential insights into the isotopic fractionations of 12CO2, 13CO2, 12C18O16O in the reaction medium utilizing integrated cavity output spectroscopy. The plasmonic system measured the reaction rate in the order of 10-7 M/s for urea species in the presence of the urease enzyme. This study deepens our understanding of plasmonic-based enzymatic urea hydrolysis in real time and opens a new way to quantify chemical reaction kinetics for various other systems. This is the first detailed experimental investigation of the real-time kinetics of urea-urease hydrolysis reaction exploiting wavelength-interrogated surface plasmon resonance method in response to produced CO2 in the CO2-free N2 environment and CO2-enriched ambient reaction medium utilizing integrated cavity output spectroscopy. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Chemical Sciences is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  – Url: https://dx.doi.org/doi:10.1007/s12039-023-02175-0
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  Data: A combined surface plasmonic and isotope-selective spectroscopic study toward a deeper understanding of real-time enzymatic urea hydrolysis.
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  Data: <searchLink fieldCode="AR" term="%22Banerjee%2C+Jayeta%22">Banerjee, Jayeta</searchLink><br /><searchLink fieldCode="AR" term="%22Pradhan%2C+Manik%22">Pradhan, Manik</searchLink>
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  Data: Journal of Chemical Sciences; Jun2023, Vol. 135 Issue 2, p1-9, 9p
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: We employed the wavelength-interrogated surface plasmon resonance (SPR) method to characterize the real-time kinetics of urea-urease hydrolysis reaction in response to a CO<subscript>2</subscript>-free N<subscript>2</subscript> environment and CO<subscript>2</subscript>-enriched ambient reaction medium. We established that a simple label-free SPR probe could accurately extract kinetic parameters from the nature of the sharp jump of the SPR wavelength shift in the reaction profile. The kinetic analysis showed that CO<subscript>2</subscript> production increases with increasing reaction time irrespective of CO<subscript>2</subscript>-free N<subscript>2</subscript> or CO<subscript>2</subscript>-enriched reaction environment. We also explored the essential insights into the isotopic fractionations of <superscript>12</superscript>CO<subscript>2</subscript>, <superscript>13</superscript>CO<subscript>2</subscript>, <superscript>12</superscript>C<superscript>18</superscript>O<superscript>16</superscript>O in the reaction medium utilizing integrated cavity output spectroscopy. The plasmonic system measured the reaction rate in the order of 10<superscript>-7</superscript> M/s for urea species in the presence of the urease enzyme. This study deepens our understanding of plasmonic-based enzymatic urea hydrolysis in real time and opens a new way to quantify chemical reaction kinetics for various other systems. This is the first detailed experimental investigation of the real-time kinetics of urea-urease hydrolysis reaction exploiting wavelength-interrogated surface plasmon resonance method in response to produced CO<subscript>2</subscript> in the CO<subscript>2</subscript>-free N<subscript>2</subscript> environment and CO<subscript>2</subscript>-enriched ambient reaction medium utilizing integrated cavity output spectroscopy. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chemical Sciences is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s12039-023-02175-0
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        Text: English
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        PageCount: 9
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      – TitleFull: A combined surface plasmonic and isotope-selective spectroscopic study toward a deeper understanding of real-time enzymatic urea hydrolysis.
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            NameFull: Banerjee, Jayeta
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            – D: 01
              M: 06
              Text: Jun2023
              Type: published
              Y: 2023
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              Value: 135
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