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Structure and Assembly of the Proteus mirabilis Flagellar Motor by Cryo-Electron Tomography.

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Title: Structure and Assembly of the Proteus mirabilis Flagellar Motor by Cryo-Electron Tomography.
Authors: Kaplan, Mohammed, Yao, Qing, Jensen, Grant J.
Source: International Journal of Molecular Sciences; May2023, Vol. 24 Issue 9, p8292, 8p
Subject Terms: Tomography, Urinary tract infections, Gram-negative bacteria, Escherichia coli
Abstract: Proteus mirabilis is a Gram-negative Gammaproteobacterium and a major causative agent of urinary tract infections in humans. It is characterized by its ability to switch between swimming motility in liquid media and swarming on solid surfaces. Here, we used cryo-electron tomography and subtomogram averaging to reveal the structure of the flagellar motor of P. mirabilis at nanometer resolution in intact cells. We found that P. mirabilis has a motor that is structurally similar to those of Escherichia coli and Salmonella enterica, lacking the periplasmic elaborations that characterize other more specialized gammaproteobacterial motors. In addition, no density corresponding to stators was present in the subtomogram average suggesting that the stators are dynamic. Finally, several assembly intermediates of the motor were seen that support the inside-out assembly pathway. [ABSTRACT FROM AUTHOR]
Copyright of International Journal of Molecular Sciences is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Label: Title
  Group: Ti
  Data: Structure and Assembly of the Proteus mirabilis Flagellar Motor by Cryo-Electron Tomography.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Kaplan%2C+Mohammed%22">Kaplan, Mohammed</searchLink><br /><searchLink fieldCode="AR" term="%22Yao%2C+Qing%22">Yao, Qing</searchLink><br /><searchLink fieldCode="AR" term="%22Jensen%2C+Grant+J%2E%22">Jensen, Grant J.</searchLink>
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  Data: International Journal of Molecular Sciences; May2023, Vol. 24 Issue 9, p8292, 8p
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Tomography%22">Tomography</searchLink><br /><searchLink fieldCode="DE" term="%22Urinary+tract+infections%22">Urinary tract infections</searchLink><br /><searchLink fieldCode="DE" term="%22Gram-negative+bacteria%22">Gram-negative bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Escherichia+coli%22">Escherichia coli</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Proteus mirabilis is a Gram-negative Gammaproteobacterium and a major causative agent of urinary tract infections in humans. It is characterized by its ability to switch between swimming motility in liquid media and swarming on solid surfaces. Here, we used cryo-electron tomography and subtomogram averaging to reveal the structure of the flagellar motor of P. mirabilis at nanometer resolution in intact cells. We found that P. mirabilis has a motor that is structurally similar to those of Escherichia coli and Salmonella enterica, lacking the periplasmic elaborations that characterize other more specialized gammaproteobacterial motors. In addition, no density corresponding to stators was present in the subtomogram average suggesting that the stators are dynamic. Finally, several assembly intermediates of the motor were seen that support the inside-out assembly pathway. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of International Journal of Molecular Sciences is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Type: general
      – SubjectFull: Urinary tract infections
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      – SubjectFull: Gram-negative bacteria
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      – TitleFull: Structure and Assembly of the Proteus mirabilis Flagellar Motor by Cryo-Electron Tomography.
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              Text: May2023
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