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Development of a strip‐based test for detection of urea adulteration in milk.

Bibliographic Details
Title: Development of a strip‐based test for detection of urea adulteration in milk.
Authors: Savaliya, Ravi R., Shaikh, Ahesanvarish Ismailbhai, Chauhan, Mahipal P., Parmar, Satishkumar Chaturbhai, Modi, Ramesh B.
Source: Journal of Food Processing & Preservation; Jul2022, Vol. 46 Issue 7, p1-10, 10p
Subject Terms: Urea, Adulterations, Milk, Detection limit, Urease, Rural geography
Geographic Terms: India
Abstract: The availability of sophisticated instrumental techniques for adulteration detection in milk is a distant dream for rural areas in India. To address this constraint, a simple chromatographic paper‐based test strip for the detection of urea in milk is developed. The said strip was prepared by dipping a chromatographic paper for 10 min in an admixture of a supernatant (coarse ground soybean powder soaked overnight in water) and an acid‐base indicator. The strip utilizes the urease naturally present in soybean for the production of ammonia from urea which consequently raises the pH enabling the change in color of the strip. The limit of detection of urea by developed strip is 0.07%. The response time of the strip ranges from 3 to 5 min. The strip was found to be stable for 6 months when stored at ambient temperature with reproducible results. Novelty impact statement: A simple, low cost and sensitive strip based test for detection of urea in milk is developed. The strip works on the principle of use of natural urease present in soybean slurry. The limit of detection observed in the test for urea detection in milk is 0.07%. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Food Processing & Preservation is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Complementary Index
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DbLabel: Complementary Index
An: 158110567
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  Label: Title
  Group: Ti
  Data: Development of a strip‐based test for detection of urea adulteration in milk.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Savaliya%2C+Ravi+R%2E%22">Savaliya, Ravi R.</searchLink><br /><searchLink fieldCode="AR" term="%22Shaikh%2C+Ahesanvarish+Ismailbhai%22">Shaikh, Ahesanvarish Ismailbhai</searchLink><br /><searchLink fieldCode="AR" term="%22Chauhan%2C+Mahipal+P%2E%22">Chauhan, Mahipal P.</searchLink><br /><searchLink fieldCode="AR" term="%22Parmar%2C+Satishkumar+Chaturbhai%22">Parmar, Satishkumar Chaturbhai</searchLink><br /><searchLink fieldCode="AR" term="%22Modi%2C+Ramesh+B%2E%22">Modi, Ramesh B.</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Journal of Food Processing & Preservation; Jul2022, Vol. 46 Issue 7, p1-10, 10p
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Urea%22">Urea</searchLink><br /><searchLink fieldCode="DE" term="%22Adulterations%22">Adulterations</searchLink><br /><searchLink fieldCode="DE" term="%22Milk%22">Milk</searchLink><br /><searchLink fieldCode="DE" term="%22Detection+limit%22">Detection limit</searchLink><br /><searchLink fieldCode="DE" term="%22Urease%22">Urease</searchLink><br /><searchLink fieldCode="DE" term="%22Rural+geography%22">Rural geography</searchLink>
– Name: SubjectGeographic
  Label: Geographic Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22India%22">India</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The availability of sophisticated instrumental techniques for adulteration detection in milk is a distant dream for rural areas in India. To address this constraint, a simple chromatographic paper‐based test strip for the detection of urea in milk is developed. The said strip was prepared by dipping a chromatographic paper for 10 min in an admixture of a supernatant (coarse ground soybean powder soaked overnight in water) and an acid‐base indicator. The strip utilizes the urease naturally present in soybean for the production of ammonia from urea which consequently raises the pH enabling the change in color of the strip. The limit of detection of urea by developed strip is 0.07%. The response time of the strip ranges from 3 to 5 min. The strip was found to be stable for 6 months when stored at ambient temperature with reproducible results. Novelty impact statement: A simple, low cost and sensitive strip based test for detection of urea in milk is developed. The strip works on the principle of use of natural urease present in soybean slurry. The limit of detection observed in the test for urea detection in milk is 0.07%. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Food Processing & Preservation is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1111/jfpp.16722
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 1
    Subjects:
      – SubjectFull: India
        Type: general
      – SubjectFull: Urea
        Type: general
      – SubjectFull: Adulterations
        Type: general
      – SubjectFull: Milk
        Type: general
      – SubjectFull: Detection limit
        Type: general
      – SubjectFull: Urease
        Type: general
      – SubjectFull: Rural geography
        Type: general
    Titles:
      – TitleFull: Development of a strip‐based test for detection of urea adulteration in milk.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Savaliya, Ravi R.
      – PersonEntity:
          Name:
            NameFull: Shaikh, Ahesanvarish Ismailbhai
      – PersonEntity:
          Name:
            NameFull: Chauhan, Mahipal P.
      – PersonEntity:
          Name:
            NameFull: Parmar, Satishkumar Chaturbhai
      – PersonEntity:
          Name:
            NameFull: Modi, Ramesh B.
    IsPartOfRelationships:
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          Dates:
            – D: 01
              M: 07
              Text: Jul2022
              Type: published
              Y: 2022
          Identifiers:
            – Type: issn-print
              Value: 01458892
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            – Type: volume
              Value: 46
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              Value: 7
          Titles:
            – TitleFull: Journal of Food Processing & Preservation
              Type: main
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