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Svep1 stabilises developmental vascular anastomosis in reduced flow conditions.

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Title: Svep1 stabilises developmental vascular anastomosis in reduced flow conditions.
Authors: Coxam, Baptiste, Collins, Russell T., Hußmann, Melina, Huisman, Yvonne, Meier, Katja, Jung, Simone, Bartels-Klein, Eireen, Szymborska, Anna, Finotto, Lise, Helker, Christian S. M., Stainier, Didier Y. R., Schulte-Merker, Stefan, Gerhardt, Holger
Source: Development (0950-1991); Mar2022, Vol. 14 Issue 6, p1-13, 13p
Subject Terms: Zebra danio embryos, Blood flow, Cellular signal transduction, Blood vessels, Brachydanio, Neovascularization
Abstract: Molecular mechanisms controlling the formation, stabilisation and maintenance of blood vessel connections remain poorly defined. Here, we identify blood flow and the large extracellular protein Svep1 as co-modulators of vessel anastomosis during developmental angiogenesis in zebrafish embryos. Both loss of Svep1 and blood flow reduction contribute to defective anastomosis of intersegmental vessels. The reduced formation and lumenisation of the dorsal longitudinal anastomotic vessel (DLAV) is associated with a compensatory increase in Vegfa/Vegfr pERK signalling, concomittant expansion of apelin-positive tip cells, but reduced expression of klf2a. Experimentally, further increasing Vegfa/Vegfr signalling can rescue the DLAV formation and lumenisation defects, whereas its inhibition dramatically exacerbates the loss of connectivity. Mechanistically, our results suggest that flow and Svep1 co-regulate the stabilisation of vascular connections, in part by modulating the Vegfa/Vegfr signalling pathway. [ABSTRACT FROM AUTHOR]
Copyright of Development (0950-1991) is the property of Company of Biologists Ltd. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Complementary Index
An: 156212033
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PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 915.917297363281
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Items – Name: Title
  Label: Title
  Group: Ti
  Data: Svep1 stabilises developmental vascular anastomosis in reduced flow conditions.
– Name: Author
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  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Coxam%2C+Baptiste%22">Coxam, Baptiste</searchLink><br /><searchLink fieldCode="AR" term="%22Collins%2C+Russell+T%2E%22">Collins, Russell T.</searchLink><br /><searchLink fieldCode="AR" term="%22Hußmann%2C+Melina%22">Hußmann, Melina</searchLink><br /><searchLink fieldCode="AR" term="%22Huisman%2C+Yvonne%22">Huisman, Yvonne</searchLink><br /><searchLink fieldCode="AR" term="%22Meier%2C+Katja%22">Meier, Katja</searchLink><br /><searchLink fieldCode="AR" term="%22Jung%2C+Simone%22">Jung, Simone</searchLink><br /><searchLink fieldCode="AR" term="%22Bartels-Klein%2C+Eireen%22">Bartels-Klein, Eireen</searchLink><br /><searchLink fieldCode="AR" term="%22Szymborska%2C+Anna%22">Szymborska, Anna</searchLink><br /><searchLink fieldCode="AR" term="%22Finotto%2C+Lise%22">Finotto, Lise</searchLink><br /><searchLink fieldCode="AR" term="%22Helker%2C+Christian+S%2E+M%2E%22">Helker, Christian S. M.</searchLink><br /><searchLink fieldCode="AR" term="%22Stainier%2C+Didier+Y%2E+R%2E%22">Stainier, Didier Y. R.</searchLink><br /><searchLink fieldCode="AR" term="%22Schulte-Merker%2C+Stefan%22">Schulte-Merker, Stefan</searchLink><br /><searchLink fieldCode="AR" term="%22Gerhardt%2C+Holger%22">Gerhardt, Holger</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Development (0950-1991); Mar2022, Vol. 14 Issue 6, p1-13, 13p
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Zebra+danio+embryos%22">Zebra danio embryos</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+flow%22">Blood flow</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+signal+transduction%22">Cellular signal transduction</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+vessels%22">Blood vessels</searchLink><br /><searchLink fieldCode="DE" term="%22Brachydanio%22">Brachydanio</searchLink><br /><searchLink fieldCode="DE" term="%22Neovascularization%22">Neovascularization</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Molecular mechanisms controlling the formation, stabilisation and maintenance of blood vessel connections remain poorly defined. Here, we identify blood flow and the large extracellular protein Svep1 as co-modulators of vessel anastomosis during developmental angiogenesis in zebrafish embryos. Both loss of Svep1 and blood flow reduction contribute to defective anastomosis of intersegmental vessels. The reduced formation and lumenisation of the dorsal longitudinal anastomotic vessel (DLAV) is associated with a compensatory increase in Vegfa/Vegfr pERK signalling, concomittant expansion of apelin-positive tip cells, but reduced expression of klf2a. Experimentally, further increasing Vegfa/Vegfr signalling can rescue the DLAV formation and lumenisation defects, whereas its inhibition dramatically exacerbates the loss of connectivity. Mechanistically, our results suggest that flow and Svep1 co-regulate the stabilisation of vascular connections, in part by modulating the Vegfa/Vegfr signalling pathway. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Development (0950-1991) is the property of Company of Biologists Ltd. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=156212033
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        Value: 10.1242/dev.199858
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      – Code: eng
        Text: English
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        PageCount: 13
        StartPage: 1
    Subjects:
      – SubjectFull: Zebra danio embryos
        Type: general
      – SubjectFull: Blood flow
        Type: general
      – SubjectFull: Cellular signal transduction
        Type: general
      – SubjectFull: Blood vessels
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      – SubjectFull: Brachydanio
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      – SubjectFull: Neovascularization
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      – TitleFull: Svep1 stabilises developmental vascular anastomosis in reduced flow conditions.
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            – D: 15
              M: 03
              Text: Mar2022
              Type: published
              Y: 2022
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