Nickel induced cell impairments are negatively regulated by the Tor1 kinase in Schizosaccharomyces pombe.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Nickel induced cell impairments are negatively regulated by the Tor1 kinase in Schizosaccharomyces pombe.
Συγγραφείς:	Navratilova, Alica, Kovar, Marek, Trakovicka, Anna, Pozgajova, Miroslava
Πηγή:	World Journal of Microbiology & Biotechnology; Oct2021, Vol. 37 Issue 10, p1-13, 13p
Θεματικοί όροι:	Schizosaccharomyces pombe, Inductively coupled plasma atomic emission spectrometry, Nickel, Cellular signal transduction, Cell growth
Περίληψη:	In our study we investigated the effect of different nickel (NiSO4·6H2O) (Ni) concentrations on cell division, cellular morphology and ionome homeostasis of the eukaryotic model organism Schizosaccharomyces pombe. Target of rapamycin (TOR) protein kinase is one of the key regulators of cell growth under different environmental stresses. We analyzed the effect of Ni on cell strains lacking the Tor1 signaling pathway utilizing light-absorbance spectroscopy, visualization, microscopy and inductively coupled plasma optical emission spectroscopy. Interestingly, our findings revealed that Ni mediated cell growth alterations are noticeably lower in Tor1 deficient cells. Greater size of Tor1 depleted cells reached similar quantitative parameters to wild type cells upon incubation with 400 μM Ni. Differences of ion levels among the two tested yeast strains were detected even before Ni addition. Addition of high concentration (1 mM) of the heavy metal, representing acute contamination, caused considerable changes in the ionome of both strains. Strikingly, Tor1 deficient cells displayed largely reduced Ni content after treatment compared to wild type controls (644.1 ± 49 vs. 2096.8 ± 75 μg/g), suggesting its significant role in Ni trafficking. Together our results predict yet undefined role for the Tor1 signaling in metal uptake and/or metabolism. [ABSTRACT FROM AUTHOR]
	Copyright of World Journal of Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Βάση Δεδομένων:	Complementary Index

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Header	DbId: edb DbLabel: Complementary Index An: 153080023 RelevancyScore: 916 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 915.652038574219
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Items	– Name: Title Label: Title Group: Ti Data: Nickel induced cell impairments are negatively regulated by the Tor1 kinase in Schizosaccharomyces pombe. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Navratilova%2C+Alica%22">Navratilova, Alica</searchLink><br /><searchLink fieldCode="AR" term="%22Kovar%2C+Marek%22">Kovar, Marek</searchLink><br /><searchLink fieldCode="AR" term="%22Trakovicka%2C+Anna%22">Trakovicka, Anna</searchLink><br /><searchLink fieldCode="AR" term="%22Pozgajova%2C+Miroslava%22">Pozgajova, Miroslava</searchLink> – Name: TitleSource Label: Source Group: Src Data: World Journal of Microbiology & Biotechnology; Oct2021, Vol. 37 Issue 10, p1-13, 13p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Schizosaccharomyces+pombe%22">Schizosaccharomyces pombe</searchLink><br /><searchLink fieldCode="DE" term="%22Inductively+coupled+plasma+atomic+emission+spectrometry%22">Inductively coupled plasma atomic emission spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Nickel%22">Nickel</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+signal+transduction%22">Cellular signal transduction</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+growth%22">Cell growth</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: In our study we investigated the effect of different nickel (NiSO<subscript>4</subscript>·6H<subscript>2</subscript>O) (Ni) concentrations on cell division, cellular morphology and ionome homeostasis of the eukaryotic model organism Schizosaccharomyces pombe. Target of rapamycin (TOR) protein kinase is one of the key regulators of cell growth under different environmental stresses. We analyzed the effect of Ni on cell strains lacking the Tor1 signaling pathway utilizing light-absorbance spectroscopy, visualization, microscopy and inductively coupled plasma optical emission spectroscopy. Interestingly, our findings revealed that Ni mediated cell growth alterations are noticeably lower in Tor1 deficient cells. Greater size of Tor1 depleted cells reached similar quantitative parameters to wild type cells upon incubation with 400 μM Ni. Differences of ion levels among the two tested yeast strains were detected even before Ni addition. Addition of high concentration (1 mM) of the heavy metal, representing acute contamination, caused considerable changes in the ionome of both strains. Strikingly, Tor1 deficient cells displayed largely reduced Ni content after treatment compared to wild type controls (644.1 ± 49 vs. 2096.8 ± 75 μg/g), suggesting its significant role in Ni trafficking. Together our results predict yet undefined role for the Tor1 signaling in metal uptake and/or metabolism. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of World Journal of Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo	BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s11274-021-03130-2 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 1 Subjects: – SubjectFull: Schizosaccharomyces pombe Type: general – SubjectFull: Inductively coupled plasma atomic emission spectrometry Type: general – SubjectFull: Nickel Type: general – SubjectFull: Cellular signal transduction Type: general – SubjectFull: Cell growth Type: general Titles: – TitleFull: Nickel induced cell impairments are negatively regulated by the Tor1 kinase in Schizosaccharomyces pombe. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Navratilova, Alica – PersonEntity: Name: NameFull: Kovar, Marek – PersonEntity: Name: NameFull: Trakovicka, Anna – PersonEntity: Name: NameFull: Pozgajova, Miroslava IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 10 Text: Oct2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 09593993 Numbering: – Type: volume Value: 37 – Type: issue Value: 10 Titles: – TitleFull: World Journal of Microbiology & Biotechnology Type: main
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