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Staphylococcus cohnii HFUTY-08: a novel acid urease-producing strain.

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Title: Staphylococcus cohnii HFUTY-08: a novel acid urease-producing strain.
Authors: Zhang, Aona, Liu, Kun, Cao, Jingjing, Yan, Wanghui, Zheng, Yue, Zeng, Qingmei
Source: Annals of Microbiology; Jun2018, Vol. 68 Issue 6, p365-374, 10p
Abstract: Urea in alcoholic beverages is a precursor of ethyl carbamate (EC), which is carcinogenic. At present, removal of urea by acid urease is considered to be the most effective method. In this study, a strain with higher acid urease production was screened and the enzyme activity was 1.12 U/mL. The strain was identified as Staphylococcus cohnii via a phylogenetic analysis of its 16S rDNA gene sequence, its morphological characteristics, and its physiological and biochemical properties, named as Staphylococcus cohnii HFUTY-08. Optimum culture conditions were determined through a single-factor test and an orthogonal test, with results as follows: glucose concentration 30 g/L, peptone concentration 15 g/L, initial pH 5, and an optimal inoculation amounts of 5%. Under these conditions, the activity of acid urease produced by strain Staphylococcus cohnii HFUTY-08 was 1.78 U/ml. Besides, the crude enzyme was purified to electrophoretic homogeneity by ion exchange chromatography and gel filtration chromatography. The molecular weight of the enzyme was estimated to be 295 kDa and the structural features of the enzyme were defined as (αβγ)3. Finally, the preliminary study on the removal of urea by acid urease in Chinese rice wine (CRW) showed that the enzyme could remove about 75% urea within 72 h at 37 °C, which effectively prevented EC production. [ABSTRACT FROM AUTHOR]
Copyright of Annals of Microbiology is the property of BioMed Central and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Staphylococcus cohnii HFUTY-08: a novel acid urease-producing strain.
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  Data: <searchLink fieldCode="AR" term="%22Zhang%2C+Aona%22">Zhang, Aona</searchLink><br /><searchLink fieldCode="AR" term="%22Liu%2C+Kun%22">Liu, Kun</searchLink><br /><searchLink fieldCode="AR" term="%22Cao%2C+Jingjing%22">Cao, Jingjing</searchLink><br /><searchLink fieldCode="AR" term="%22Yan%2C+Wanghui%22">Yan, Wanghui</searchLink><br /><searchLink fieldCode="AR" term="%22Zheng%2C+Yue%22">Zheng, Yue</searchLink><br /><searchLink fieldCode="AR" term="%22Zeng%2C+Qingmei%22">Zeng, Qingmei</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: Annals of Microbiology; Jun2018, Vol. 68 Issue 6, p365-374, 10p
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Urea in alcoholic beverages is a precursor of ethyl carbamate (EC), which is carcinogenic. At present, removal of urea by acid urease is considered to be the most effective method. In this study, a strain with higher acid urease production was screened and the enzyme activity was 1.12 U/mL. The strain was identified as Staphylococcus cohnii via a phylogenetic analysis of its 16S rDNA gene sequence, its morphological characteristics, and its physiological and biochemical properties, named as Staphylococcus cohnii HFUTY-08. Optimum culture conditions were determined through a single-factor test and an orthogonal test, with results as follows: glucose concentration 30 g/L, peptone concentration 15 g/L, initial pH 5, and an optimal inoculation amounts of 5%. Under these conditions, the activity of acid urease produced by strain Staphylococcus cohnii HFUTY-08 was 1.78 U/ml. Besides, the crude enzyme was purified to electrophoretic homogeneity by ion exchange chromatography and gel filtration chromatography. The molecular weight of the enzyme was estimated to be 295 kDa and the structural features of the enzyme were defined as (αβγ)<subscript>3</subscript>. Finally, the preliminary study on the removal of urea by acid urease in Chinese rice wine (CRW) showed that the enzyme could remove about 75% urea within 72 h at 37 °C, which effectively prevented EC production. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Annals of Microbiology is the property of BioMed Central and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1007/s13213-018-1343-1
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      – TitleFull: Staphylococcus cohnii HFUTY-08: a novel acid urease-producing strain.
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            NameFull: Zhang, Aona
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              Text: Jun2018
              Type: published
              Y: 2018
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