Academic Journal
Immobilization of urease onto cellulose spheres for the selective removal of urea.
| Title: | Immobilization of urease onto cellulose spheres for the selective removal of urea. |
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| Authors: | Lv, Miaomiao, Ma, Xiaofei, Anderson, Debbie P., Chang, Peter R. |
| Source: | Cellulose; Jan2018, Vol. 25 Issue 1, p233-243, 11p |
| Subject Terms: | Urease genetics, Amidases, Cellulose synthase, Crystallinity, Crystal structure |
| Abstract: | Cellulose spheres were selected as an economical and biodegradable carrier for urease immobilization. The high crystallinity cellulose spheres (Ces) were crosslinked using citric acid and then oxidized using sodium periodate to produce aldehyde groups on the Ces surface that would bind covalently with the amino groups of urease through a Schiff base reaction. Ces, the obtained dialdehyde cellulose spheres (DACes) and cellulose spheres immobilized by urease (UrCes) were characterized by FTIR, XRD, SEM and TG. The results demonstrated the successful modification of Ces and immobilization of urease, along with the retention of the original spherical morphology and improved thermal stability. As an adsorbent, UrCes had both a greater adsorption capacity for urea and a greater specific selective adsorption than DACes. Urease was respectively immobilized on DACes with 19, 31 and 45% aldehyde contents to obtain UrCes1, UrCes2 and UrCes3. The maximum removal capacities of UrCes1, UrCes2, and UrCes3 for urea were 243.3, 276.2, and 187.6 mg g, while only 10.71, 15.08 and 20.50 mg g l-phenylalanine were adsorbed, respectively. [ABSTRACT FROM AUTHOR] |
| Copyright of Cellulose is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Complementary Index |
| FullText | Links: – Type: other Text: Availability: 0 CustomLinks: – Url: https://dx.doi.org/doi:10.1007/s10570-017-1592-3 Name: EDS - Springer Nature Journals (s7799221) Category: fullText Text: View record at Springer |
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| Header | DbId: edb DbLabel: Complementary Index An: 127247653 RelevancyScore: 862 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 861.622375488281 |
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| Items | – Name: Title Label: Title Group: Ti Data: Immobilization of urease onto cellulose spheres for the selective removal of urea. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Lv%2C+Miaomiao%22">Lv, Miaomiao</searchLink><br /><searchLink fieldCode="AR" term="%22Ma%2C+Xiaofei%22">Ma, Xiaofei</searchLink><br /><searchLink fieldCode="AR" term="%22Anderson%2C+Debbie+P%2E%22">Anderson, Debbie P.</searchLink><br /><searchLink fieldCode="AR" term="%22Chang%2C+Peter+R%2E%22">Chang, Peter R.</searchLink> – Name: TitleSource Label: Source Group: Src Data: Cellulose; Jan2018, Vol. 25 Issue 1, p233-243, 11p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Urease+genetics%22">Urease genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Amidases%22">Amidases</searchLink><br /><searchLink fieldCode="DE" term="%22Cellulose+synthase%22">Cellulose synthase</searchLink><br /><searchLink fieldCode="DE" term="%22Crystallinity%22">Crystallinity</searchLink><br /><searchLink fieldCode="DE" term="%22Crystal+structure%22">Crystal structure</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Cellulose spheres were selected as an economical and biodegradable carrier for urease immobilization. The high crystallinity cellulose spheres (Ces) were crosslinked using citric acid and then oxidized using sodium periodate to produce aldehyde groups on the Ces surface that would bind covalently with the amino groups of urease through a Schiff base reaction. Ces, the obtained dialdehyde cellulose spheres (DACes) and cellulose spheres immobilized by urease (UrCes) were characterized by FTIR, XRD, SEM and TG. The results demonstrated the successful modification of Ces and immobilization of urease, along with the retention of the original spherical morphology and improved thermal stability. As an adsorbent, UrCes had both a greater adsorption capacity for urea and a greater specific selective adsorption than DACes. Urease was respectively immobilized on DACes with 19, 31 and 45% aldehyde contents to obtain UrCes1, UrCes2 and UrCes3. The maximum removal capacities of UrCes1, UrCes2, and UrCes3 for urea were 243.3, 276.2, and 187.6 mg g, while only 10.71, 15.08 and 20.50 mg g l-phenylalanine were adsorbed, respectively. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Cellulose is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10570-017-1592-3 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 233 Subjects: – SubjectFull: Urease genetics Type: general – SubjectFull: Amidases Type: general – SubjectFull: Cellulose synthase Type: general – SubjectFull: Crystallinity Type: general – SubjectFull: Crystal structure Type: general Titles: – TitleFull: Immobilization of urease onto cellulose spheres for the selective removal of urea. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Lv, Miaomiao – PersonEntity: Name: NameFull: Ma, Xiaofei – PersonEntity: Name: NameFull: Anderson, Debbie P. – PersonEntity: Name: NameFull: Chang, Peter R. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2018 Type: published Y: 2018 Identifiers: – Type: issn-print Value: 09690239 Numbering: – Type: volume Value: 25 – Type: issue Value: 1 Titles: – TitleFull: Cellulose Type: main |
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