Effects of variability in anatomical reconstruction techniques on models of synaptic integration by dendrites: a comparison of three internet archives.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Effects of variability in anatomical reconstruction techniques on models of synaptic integration by dendrites: a comparison of three internet archives.
Συγγραφείς:	Szilágyi, Tibor, De Schutter, Erik
Πηγή:	European Journal of Neuroscience; Mar2004, Vol. 19 Issue 5, p1257-1266, 10p
Θεματικοί όροι:	Neurons, Dynamics, Synapses
Περίληψη:	The first step in building a realistic computational neuron model is to produce a passive electrical skeleton on to which active conductances can be grafted. For this, anatomically accurate morphological reconstructions of the desired cell type are required. In this study compartmental models were used to compare from a functional perspective three on-line archives of rat hippocampal CA1 pyramidal cell morphologies. The topological organization of cells was found to be similar for all archives, but several morphometric differences were observed. The three-dimensional size of the cells, the diameter and tortuosity of dendrites, and the electrotonic length of the main apical dendrite and of the branches in stratum lacunosum moleculare were dissimilar. The experimentally measured kinetics of somatically recorded inhibitory postsynaptic currents evoked in the stratum lacunosum moleculare (data from the literature) could be reproduced only using the archives that contained cells with an electrotonically short main apical dendrite. In the amplitude attenuation of the simulated postsynaptic currents and the voltage escape from the command potential under voltage clamp conditions, a two- to three-fold difference was observed among archives. Upon activation of a single model synapse on distal branches, cells with low dendritic diameter showed a voltage escape larger than 15 mV. The diameter of the dendrites influenced greatly the results, emphasizing the importance of methods that allow an accurate measurement of this parameter. Our results indicate that there are functionally significant differences in the morphometric data available in different archives even if the cell type, brain region and species are the same. [ABSTRACT FROM AUTHOR]
	Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Βάση Δεδομένων:	Complementary Index

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Header	DbId: edb DbLabel: Complementary Index An: 12493013 RelevancyScore: 832 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 832.050415039063
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Items	– Name: Title Label: Title Group: Ti Data: Effects of variability in anatomical reconstruction techniques on models of synaptic integration by dendrites: a comparison of three internet archives. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Szilágyi%2C+Tibor%22">Szilágyi, Tibor</searchLink><br /><searchLink fieldCode="AR" term="%22De+Schutter%2C+Erik%22">De Schutter, Erik</searchLink> – Name: TitleSource Label: Source Group: Src Data: European Journal of Neuroscience; Mar2004, Vol. 19 Issue 5, p1257-1266, 10p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Neurons%22">Neurons</searchLink><br /><searchLink fieldCode="DE" term="%22Dynamics%22">Dynamics</searchLink><br /><searchLink fieldCode="DE" term="%22Synapses%22">Synapses</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The first step in building a realistic computational neuron model is to produce a passive electrical skeleton on to which active conductances can be grafted. For this, anatomically accurate morphological reconstructions of the desired cell type are required. In this study compartmental models were used to compare from a functional perspective three on-line archives of rat hippocampal CA1 pyramidal cell morphologies. The topological organization of cells was found to be similar for all archives, but several morphometric differences were observed. The three-dimensional size of the cells, the diameter and tortuosity of dendrites, and the electrotonic length of the main apical dendrite and of the branches in stratum lacunosum moleculare were dissimilar. The experimentally measured kinetics of somatically recorded inhibitory postsynaptic currents evoked in the stratum lacunosum moleculare (data from the literature) could be reproduced only using the archives that contained cells with an electrotonically short main apical dendrite. In the amplitude attenuation of the simulated postsynaptic currents and the voltage escape from the command potential under voltage clamp conditions, a two- to three-fold difference was observed among archives. Upon activation of a single model synapse on distal branches, cells with low dendritic diameter showed a voltage escape larger than 15 mV. The diameter of the dendrites influenced greatly the results, emphasizing the importance of methods that allow an accurate measurement of this parameter. Our results indicate that there are functionally significant differences in the morphometric data available in different archives even if the cell type, brain region and species are the same. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of European Journal of Neuroscience is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo	BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/j.1460-9568.2004.03222.x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 1257 Subjects: – SubjectFull: Neurons Type: general – SubjectFull: Dynamics Type: general – SubjectFull: Synapses Type: general Titles: – TitleFull: Effects of variability in anatomical reconstruction techniques on models of synaptic integration by dendrites: a comparison of three internet archives. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Szilágyi, Tibor – PersonEntity: Name: NameFull: De Schutter, Erik IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2004 Type: published Y: 2004 Identifiers: – Type: issn-print Value: 0953816X Numbering: – Type: volume Value: 19 – Type: issue Value: 5 Titles: – TitleFull: European Journal of Neuroscience Type: main
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