Academic Journal
Adipose triglyceride lipase: function, regulation by insulin, and comparison with adiponutrin.
| Τίτλος: | Adipose triglyceride lipase: function, regulation by insulin, and comparison with adiponutrin. |
|---|---|
| Συγγραφείς: | Kershaw EE, Hamm JK, Verhagen LAW, Peroni O, Katic M, Flier JS, Kershaw, Erin E, Hamm, Jonathan K, Verhagen, Linda A W, Peroni, Odile, Katic, Masa, Flier, Jeffrey S |
| Πηγή: | Diabetes; Jan2006, Vol. 55 Issue 1, p148-157, 10p |
| Περίληψη: | Adipose triglyceride lipase (ATGL) is a recently described adipose-enriched protein with triglyceride-specific lipase activity. ATGL shares the greatest sequence homology with adiponutrin, a nutritionally regulated protein of unclear biological function. Here we present a functional analysis of ATGL and adiponutrin and describe their regulation by insulin. Retroviral-mediated overexpression of ATGL in 3T3-L1 adipocytes increased basal and isoproterenol-stimulated glycerol and nonesterified fatty acid (NEFA) release, whereas siRNA-mediated knockdown of ATGL had the opposite effect. In contrast, siRNA-mediated knockdown of adiponutrin in 3T3-L1 adipocytes had no effect on glycerol or NEFA release. In mice, both ATGL and adiponutrin are nutritionally regulated in adipose tissue, with ATGL being upregulated and adiponutrin being downregulated by fasting. In 3T3-L1 adipocytes, insulin decreased ATGL and increased adiponutrin expression in a dose- and time-dependent manner, suggesting that insulin directly mediates this nutritional regulation. In addition, adipose expression of ATGL was increased by insulin deficiency and decreased by insulin replacement in streptozotocin-induced diabetic mice and was increased in fat-specific insulin receptor knockout mice, whereas adiponutrin showed the opposite pattern. These data suggest that murine ATGL but not adiponutrin contributes to net adipocyte lipolysis and that ATGL and adiponutrin are oppositely regulated by insulin both in vitro and in vivo. [ABSTRACT FROM AUTHOR] |
| Copyright of Diabetes is the property of American Diabetes Association and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Βάση Δεδομένων: | Complementary Index |
| FullText | Text: Availability: 0 |
|---|---|
| Header | DbId: edb DbLabel: Complementary Index An: 106453682 RelevancyScore: 833 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 832.6142578125 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Adipose triglyceride lipase: function, regulation by insulin, and comparison with adiponutrin. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kershaw+EE%22">Kershaw EE</searchLink><br /><searchLink fieldCode="AR" term="%22Hamm+JK%22">Hamm JK</searchLink><br /><searchLink fieldCode="AR" term="%22Verhagen+LAW%22">Verhagen LAW</searchLink><br /><searchLink fieldCode="AR" term="%22Peroni+O%22">Peroni O</searchLink><br /><searchLink fieldCode="AR" term="%22Katic+M%22">Katic M</searchLink><br /><searchLink fieldCode="AR" term="%22Flier+JS%22">Flier JS</searchLink><br /><searchLink fieldCode="AR" term="%22Kershaw%2C+Erin+E%22">Kershaw, Erin E</searchLink><br /><searchLink fieldCode="AR" term="%22Hamm%2C+Jonathan+K%22">Hamm, Jonathan K</searchLink><br /><searchLink fieldCode="AR" term="%22Verhagen%2C+Linda+A+W%22">Verhagen, Linda A W</searchLink><br /><searchLink fieldCode="AR" term="%22Peroni%2C+Odile%22">Peroni, Odile</searchLink><br /><searchLink fieldCode="AR" term="%22Katic%2C+Masa%22">Katic, Masa</searchLink><br /><searchLink fieldCode="AR" term="%22Flier%2C+Jeffrey+S%22">Flier, Jeffrey S</searchLink> – Name: TitleSource Label: Source Group: Src Data: Diabetes; Jan2006, Vol. 55 Issue 1, p148-157, 10p – Name: Abstract Label: Abstract Group: Ab Data: Adipose triglyceride lipase (ATGL) is a recently described adipose-enriched protein with triglyceride-specific lipase activity. ATGL shares the greatest sequence homology with adiponutrin, a nutritionally regulated protein of unclear biological function. Here we present a functional analysis of ATGL and adiponutrin and describe their regulation by insulin. Retroviral-mediated overexpression of ATGL in 3T3-L1 adipocytes increased basal and isoproterenol-stimulated glycerol and nonesterified fatty acid (NEFA) release, whereas siRNA-mediated knockdown of ATGL had the opposite effect. In contrast, siRNA-mediated knockdown of adiponutrin in 3T3-L1 adipocytes had no effect on glycerol or NEFA release. In mice, both ATGL and adiponutrin are nutritionally regulated in adipose tissue, with ATGL being upregulated and adiponutrin being downregulated by fasting. In 3T3-L1 adipocytes, insulin decreased ATGL and increased adiponutrin expression in a dose- and time-dependent manner, suggesting that insulin directly mediates this nutritional regulation. In addition, adipose expression of ATGL was increased by insulin deficiency and decreased by insulin replacement in streptozotocin-induced diabetic mice and was increased in fat-specific insulin receptor knockout mice, whereas adiponutrin showed the opposite pattern. These data suggest that murine ATGL but not adiponutrin contributes to net adipocyte lipolysis and that ATGL and adiponutrin are oppositely regulated by insulin both in vitro and in vivo. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Diabetes is the property of American Diabetes Association and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=106453682 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.2337/diabetes.55.01.06.db05-0982 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 148 Titles: – TitleFull: Adipose triglyceride lipase: function, regulation by insulin, and comparison with adiponutrin. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kershaw EE – PersonEntity: Name: NameFull: Hamm JK – PersonEntity: Name: NameFull: Verhagen LAW – PersonEntity: Name: NameFull: Peroni O – PersonEntity: Name: NameFull: Katic M – PersonEntity: Name: NameFull: Flier JS – PersonEntity: Name: NameFull: Kershaw, Erin E – PersonEntity: Name: NameFull: Hamm, Jonathan K – PersonEntity: Name: NameFull: Verhagen, Linda A W – PersonEntity: Name: NameFull: Peroni, Odile – PersonEntity: Name: NameFull: Katic, Masa – PersonEntity: Name: NameFull: Flier, Jeffrey S IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Text: Jan2006 Type: published Y: 2006 Identifiers: – Type: issn-print Value: 00121797 Numbering: – Type: volume Value: 55 – Type: issue Value: 1 Titles: – TitleFull: Diabetes Type: main |
| ResultId | 1 |