Academic Journal
Evaluation of a new reagent strip rapid urease test for detection of Helicobacter pylori infection.
| Τίτλος: | Evaluation of a new reagent strip rapid urease test for detection of Helicobacter pylori infection. |
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| Συγγραφείς: | Yousfi MM; Department of Medicine, Veterans Affairs Medical Center, Houston, Texas 77030, USA., el-Zimaity HM, Genta RM, Graham DY |
| Πηγή: | Gastrointestinal endoscopy [Gastrointest Endosc] 1996 Nov; Vol. 44 (5), pp. 519-22. |
| Τύπος έκδοσης: | Clinical Trial; Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Mosby Yearbook Country of Publication: United States NLM ID: 0010505 Publication Model: Print Cited Medium: Print ISSN: 0016-5107 (Print) Linking ISSN: 00165107 NLM ISO Abbreviation: Gastrointest Endosc Subsets: MEDLINE |
| Imprint Name(s): | Publication: St Louis, Mo : Mosby Yearbook Original Publication: Denver. |
| Ιατρικοί όροι (MeSH): | Reagent Strips*, Helicobacter Infections/*microbiology , Helicobacter pylori/*isolation & purification , Urease/*analysis, Gastric Mucosa/microbiology ; Helicobacter pylori/enzymology ; Pyloric Antrum/microbiology ; Adult ; Agar ; Aged ; Aged, 80 and over ; Biopsy ; Evaluation Studies as Topic ; False Negative Reactions ; False Positive Reactions ; Female ; Gels ; Humans ; Male ; Middle Aged |
| Περίληψη: | Background: Rapid urease tests are commonly used as a convenient method to detect Helicobacter pylori infection. Our previous experiments demonstrated enhanced efficacy of agar gel rapid urease test compared with reagent strip rapid urease tests. We evaluated the efficacy of PyloriTek, a new reagent strip rapid test for detecting H. pylori infection. Methods: Gastric antral mucosal biopsy specimens were obtained for comparison between agar gel rapid urease tests and PyloriTek (200 specimens). The rapid urease test to be used first was selected randomly. H. pylori status was determined using the Genta stain. Culture was performed to confirm H. pylori status when false rapid urease tests were suspected. Results: One hundred patients were studied; 68 had H. pylori infection. There were two false-negative and one false-positive PyloriTek when scored at 1 hour, compared with only one false-positive and no false-negative tests at 2 hours. With the agar gel rapid urease tests, there were no false-positive tests and 5 false-negative tests when scored at 1 hour, 2 false-negative tests at 12 hours and 1 at 24 hours; there were no false-positive tests. At 1 hour, 3% (95% CI = 1% to 9%) of PyloriTek tests had an erroneous categorization of H. pylori status compared with 5% for the agar gel rapid urease tests (95% CI = 1.6% to 11%) (p > 0.7). Conclusion: The new reagent strip rapid urease test, PyloriTek, is rapid and comparable in accuracy to agar gel rapid urease tests for detecting H. pylori Infection. |
| Substance Nomenclature: | 0 (Gels) 0 (Reagent Strips) 9002-18-0 (Agar) EC 3.5.1.5 (Urease) |
| Entry Date(s): | Date Created: 19961101 Date Completed: 19970304 Latest Revision: 20190722 |
| Update Code: | 20260130 |
| DOI: | 10.1016/s0016-5107(96)70001-9 |
| PMID: | 8934154 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 0016-5107 |
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| DOI: | 10.1016/s0016-5107(96)70001-9 |