Academic Journal

Organ-Specific Phenolic Specialization and Antioxidant Responses in Crocus cancellatus: LC-MS/MS and Multivariate Analysis.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Organ-Specific Phenolic Specialization and Antioxidant Responses in Crocus cancellatus: LC-MS/MS and Multivariate Analysis.
Συγγραφείς: Selvi B; Department of Biology, Faculty of Arts and Sciences, Tokat Gaziosmanpaşa University, 60250 Tokat, Türkiye.
Πηγή: International journal of molecular sciences [Int J Mol Sci] 2026 Oct 07; Vol. 27 (19). Date of Electronic Publication: 2026 Oct 07.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
Imprint Name(s): Original Publication: Basel, Switzerland : MDPI, [2000-
Ιατρικοί όροι (MeSH): Antioxidants*/chemistry , Antioxidants*/pharmacology , Phenols*/chemistry , Phenols*/analysis , Phenols*/pharmacology , Crocus*/chemistry , Crocus*/metabolism, Tandem Mass Spectrometry/methods ; Liquid Chromatography-Mass Spectrometry/methods ; Plant Extracts/chemistry ; Flavonoids/chemistry ; Flowers/chemistry ; Multivariate Analysis ; Chromatography, Liquid ; Organ Specificity ; Principal Component Analysis
Περίληψη: Organ-specific allocation of phenolic metabolites may shape antioxidant responses in Crocus species. This study characterized stamen, pistil, tepal, perigon tube, and corm of Crocus cancellatus Herb. using ultrasound-assisted methanolic extraction, liquid chromatography-tandem mass spectrometry with 30 authentic standards, six antioxidant assays, a relative antioxidant capacity index, Pearson correlation analysis, and principal component analysis. Twenty phenolic compounds were quantified. Hesperidin predominated in all organs and was highest in the tepal (17,660 ± 60 μg/g extract), approximately 43-fold above the corm. The pistil contained the highest 3-hydroxybenzoic acid (331 ± 1 μg/g extract) and 4-hydroxybenzoic acid (341 ± 2 μg/g extract), whereas the corm was enriched in pinoresinol (60.1 ± 0.8 μg/g extract) and uniquely contained vanillin (16.0 ± 0.6 μg/g extract). The tepal showed the highest total flavonoid content (81.50 ± 0.40 mg RE/g extract), while the stamen had the highest total phenolic content (12.63 ± 0.05 mg GAE/g extract). The corm showed the strongest DPPH radical scavenging (IC50 = 3.96 mg/mL), whereas the pistil led in ABTS and FRAP responses. Principal component analysis explained 64.16% of total variance and separated the five organ profiles. These findings demonstrate organ-specific phenolic specialization and support targeted bioactivity studies.
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Contributed Indexing: Keywords: Crocus cancellatus; LC–MS/MS; antioxidant capacity; hesperidin; multivariate analysis; organ-specific phytochemistry; phenolic compounds; principal component analysis
Substance Nomenclature: 0 (Antioxidants)
0 (Phenols)
0 (Plant Extracts)
0 (Flavonoids)
Entry Date(s): Date Created: 20261016 Date Completed: 20261016 Latest Revision: 20261020
Update Code: 20261020
PubMed Central ID: PMC13664975
DOI: 10.3390/ijms27198904
PMID: 42884146
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1422-0067
DOI:10.3390/ijms27198904