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Plasmid-Host Networks Reveal Persistent Mobile Elements Across Wastewater Treatment.

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Τίτλος: Plasmid-Host Networks Reveal Persistent Mobile Elements Across Wastewater Treatment.
Συγγραφείς: Zhou S; Civil and Environmental Engineering, Rice University, Houston, TX, USA., Philo SE; Sonny Astani Department of Civil and Environmental Engineering, University of Southern California, Los Angeles, CA, USA., Saldana MA; Sonny Astani Department of Civil and Environmental Engineering, University of Southern California, Los Angeles, CA, USA., Vela JD; Department of Civil and Environmental Engineering, Duke University, Durham, NC, USA., Smith AL; Sonny Astani Department of Civil and Environmental Engineering, University of Southern California, Los Angeles, CA, USA., Stadler LB; Civil and Environmental Engineering, Rice University, Houston, TX, USA. lauren.stadler@rice.edu.
Πηγή: Nature communications [Nat Commun] 2026 Sep 07; Vol. 17 (1). Date of Electronic Publication: 2026 Sep 07.
Τύπος έκδοσης: Journal Article; Research Support, Non-U.S. Gov't
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
Imprint Name(s): Original Publication: [London] : Nature Pub. Group
Ιατρικοί όροι (MeSH): Plasmids*/genetics , Wastewater*/microbiology , Interspersed Repetitive Sequences*/genetics , Water Purification*, Sewage/microbiology ; Anti-Bacterial Agents/pharmacology ; Drug Resistance, Bacterial/genetics ; Host Specificity/genetics ; Bacteria/genetics ; Gene Transfer, Horizontal ; Metagenomics
Περίληψη: Plasmids drive horizontal gene transfer and antimicrobial resistance spread in water resource recovery facilities, yet their in situ dynamics in complex microbial communities remain poorly characterized. We apply Hi-C metagenomics to resolve plasmid-host associations across influent, activated sludge, and effluent at three facilities. We identify 944 plasmid clusters with facility- and stage-specific distributions. Plasmid host range narrows from influent to effluent, indicating that treatment selectively constrains plasmid-host associations. Persistent plasmid clusters with detectable mobility markers show a trend toward broader, less modular host networks than those with no detected markers. Hi-C-supported antibiotic resistance gene associations highlight Burkholderiaceae and Rhodocyclaceae as prominent hosts of beta-lactam, tetracycline and sulfonamide resistance genes across facilities. These findings indicate that wastewater treatment narrows plasmid host range while maintaining dominant plasmid-carrying taxa and adaptive mobile elements, providing a framework for understanding plasmid persistence across treatment systems.
(© 2026. The Author(s).)
Competing Interests: Competing interests: The authors declare no competing interests.
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Grant Information: No. EEC-2133504 National Science Foundation (NSF); R21 AI190938 United States AI NIAID NIH HHS; W9132T-23-2-0002 United States Department of Defense | United States Army | U.S. Army Corps of Engineers (US Army Corps of Engineers); R21AI190938 U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID); NAS Grant G10001728 National Academies of Sciences, Engineering, and Medicine | National Academy of Sciences (NAS); W9132T-23-2-0002 United States Department of Defense | United States Army | US Army Corps of Engineers | Engineer Research and Development Center (U.S. Army Engineer Research and Development Center)
Substance Nomenclature: 0 (Wastewater)
0 (Sewage)
0 (Anti-Bacterial Agents)
Entry Date(s): Date Created: 20261007 Date Completed: 20261007 Latest Revision: 20261010
Update Code: 20261010
PubMed Central ID: PMC13646282
DOI: 10.1038/s41467-026-77447-7
PMID: 42844282
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:2041-1723
DOI:10.1038/s41467-026-77447-7