Academic Journal
A Direct DNA Extraction Workflow for Metabarcoding Fungal Bioaerosols From Adhesive Samplers.
| Τίτλος: | A Direct DNA Extraction Workflow for Metabarcoding Fungal Bioaerosols From Adhesive Samplers. |
|---|---|
| Συγγραφείς: | Jung J; Department of Infectious Disease Epidemiology, School of Public Health, Imperial College London, London, UK., Leitao R; Department of Infectious Disease Epidemiology, School of Public Health, Imperial College London, London, UK., Day ALF; Department of Infectious Disease Epidemiology, School of Public Health, Imperial College London, London, UK., Davis H; Niab, Cambridge, UK., Hawkins N; Niab, Cambridge, UK., Kanyuka K; Niab, Cambridge, UK., Fisher MC; Department of Infectious Disease Epidemiology, School of Public Health, Imperial College London, London, UK. |
| Πηγή: | Environmental microbiology [Environ Microbiol] 2026 Oct; Vol. 28 (10), pp. e70431. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Blackwell Science Country of Publication: England NLM ID: 100883692 Publication Model: Print Cited Medium: Internet ISSN: 1462-2920 (Electronic) Linking ISSN: 14622912 NLM ISO Abbreviation: Environ Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Oxford : Blackwell Science, 1999- |
| Ιατρικοί όροι (MeSH): | Fungi*/genetics , Fungi*/isolation & purification , Fungi*/classification , DNA, Fungal*/isolation & purification , DNA, Fungal*/genetics , DNA Barcoding, Taxonomic*/methods , Environmental Monitoring*/methods , Environmental Monitoring*/instrumentation , Air Microbiology*, Aerosols/analysis ; Adhesives ; Workflow |
| Περίληψη: | Monitoring fungal bioaerosols is essential for understanding their impacts on human health and crop productivity. However, current monitoring approaches rely on culture-dependent techniques, which underestimate the diversity of fungal exposures. Adhesive-coated films can effectively capture airborne particles but have been regarded as unsuitable for downstream DNA analysis, owing to the lack of standardised DNA extraction methods for the adhesive capture surface. Here, we present an optimised DNA extraction protocol for adhesive film, which we developed and validated to enable direct and culture-independent ITS2-based metabarcoding of fungal bioaerosols collected on a 96-well plate sealing film ('Sticky sampler'). This validated approach was applied to field samples collected from four agricultural farms and from a domestic garden to evaluate seasonal and spatial changes in fungal bioaerosols. Our analysis uncovered pronounced site-specific contrasts linked to geography and land management, as well as clear seasonal shifts in garden fungal communities. This study demonstrates that adhesive samplers, combined with optimised DNA extraction, provide a practical and inexpensive tool for fungal bioaerosol research by resolving ecologically meaningful spatiotemporal dynamics of airborne fungi. This protocol opens new opportunities for highly scalable surveillance of environmental bioaerosols. (© 2026 The Author(s). Environmental Microbiology published by John Wiley & Sons Ltd.) |
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| Grant Information: | MR/X020258/1 United Kingdom MRC_ Medical Research Council; NE/X00547X/1 Natural Environment Research Council (NERC); United Kingdom WT_ Wellcome Trust; 219551/Z/19/Z United Kingdom WT_ Wellcome Trust; NE/X005259/1 Natural Environment Research Council (NERC) |
| Contributed Indexing: | Keywords: DNA extraction; ITS2 metabarcoding; adhesive samplers; airborne fungal communities; culture‐independent methods; environmental monitoring; fungal bioaerosols |
| Substance Nomenclature: | 0 (DNA, Fungal) 0 (Aerosols) 0 (Adhesives) |
| Entry Date(s): | Date Created: 20261006 Date Completed: 20261006 Latest Revision: 20261009 |
| Update Code: | 20261009 |
| PubMed Central ID: | PMC13641972 |
| DOI: | 10.1111/1462-2920.70431 |
| PMID: | 42838728 |
| Βάση Δεδομένων: | MEDLINE |
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