Academic Journal
Προβολή σε EDS

The phenotypic landscape of the model firmicute Bacillus subtilis.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: The phenotypic landscape of the model firmicute Bacillus subtilis.
Συγγραφείς: Kim LM; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158., Koo BM; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158., Burkhart HN; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158., Vollmer D; Institute for Molecular Bioscience, The University of Queensland, Brisbane 4067, QLD, Australia., Vollmer W; Institute for Molecular Bioscience, The University of Queensland, Brisbane 4067, QLD, Australia., Todor H; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158., Gross CA; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158.; Department of Cell and Tissue Biology, University of California, San Francisco, CA 94158.; Quantitative Biosciences Institute, University of California, San Francisco, CA 94158.
Πηγή: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2026 Oct 06; Vol. 123 (40), pp. e2619440123. Date of Electronic Publication: 2026 Sep 29.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE
Imprint Name(s): Original Publication: Washington, DC : National Academy of Sciences
Ιατρικοί όροι (MeSH): Bacillus subtilis*/genetics , Bacillus subtilis*/metabolism , Bacillus subtilis*/growth & development, Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cell Wall/metabolism ; Phenotype ; Genome, Bacterial
Περίληψη: Firmicutes are gram-positive bacteria with important roles in human health, disease, and industry. However, more than a quarter of genes in the model firmicute Bacillus subtilis remain completely uncharacterized, including numerous phylum-specific genes. Here, we design a compact pooled CRISPRi library targeting all protein-coding genes in B. subtilis and test its growth in ~150 stress conditions. Using data from this screen as a hypothesis generator, we perform targeted experiments, revealing that the conserved essential firmicute protein YneF is part of the SRP cotranslational protein secretion pathway. We also demonstrate that ECF-transporters play a broadly conserved role in cell wall homeostasis, perform an unbiased analysis of amino acid crossfeeding, and make additional insights into bacterial competition. This work additionally provides a rich dataset to nucleate future studies of uncharacterized genes and presents a framework for performing accessible full-genome functional genomic screens in other bacteria.
Competing Interests: Competing interests statement:The authors declare no competing interest.
Σχόλια: Update of: bioRxiv. 2026 Jun 01:2026.05.13.724699. doi: 10.64898/2026.05.13.724699.. (PMID: 42182432)
References: J Biol Chem. 2013 Dec 20;288(51):36385-97. (PMID: 24151069)
J Bacteriol. 2025 Oct 23;207(10):e0022025. (PMID: 40919935)
Cell. 2016 Jun 2;165(6):1493-1506. (PMID: 27238023)
Mol Syst Biol. 2015 Nov 17;11(11):839. (PMID: 26577401)
Mol Microbiol. 2014 Sep;93(6):1284-301. (PMID: 25069588)
Cell Syst. 2020 Nov 18;11(5):523-535.e9. (PMID: 33080209)
Annu Rev Biochem. 2019 Jun 20;88:551-576. (PMID: 30485755)
Mol Microbiol. 2006 May;60(3):765-82. (PMID: 16629676)
Mol Syst Biol. 2026 Apr;22(4):497-518. (PMID: 41559189)
Rev Med Microbiol. 2016 Jul;27(3):95-101. (PMID: 27340340)
J Bacteriol. 1999 Apr;181(7):2059-66. (PMID: 10094682)
Appl Environ Microbiol. 2024 Jan 24;90(1):e0156623. (PMID: 38126758)
Antimicrob Agents Chemother. 1999 May;43(5):1124-8. (PMID: 10223924)
J Bacteriol. 2005 Sep;187(17):5946-54. (PMID: 16109936)
J Cell Biol. 2003 May 26;161(4):679-84. (PMID: 12756233)
ISME J. 2023 Dec;17(12):2370-2380. (PMID: 37891427)
Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):425-30. (PMID: 16407165)
mBio. 2024 Nov 13;15(11):e0231024. (PMID: 39440976)
Biochem Biophys Res Commun. 1970 May 22;39(4):699-706. (PMID: 4992428)
EMBO Rep. 2025 Sep;26(18):4587-4606. (PMID: 40817173)
Microbiology (Reading). 2002 Nov;148(Pt 11):3441-3455. (PMID: 12427936)
Nat Rev Microbiol. 2024 Sep;22(9):556-571. (PMID: 38730101)
Mol Syst Biol. 2023 Apr 12;19(4):e11544. (PMID: 36815589)
Nat Commun. 2024 Sep 9;15(1):7866. (PMID: 39251624)
Cell. 2014 Dec 4;159(6):1300-11. (PMID: 25480295)
Mol Syst Biol. 2024 Nov;20(11):1173-1186. (PMID: 39349762)
ISME J. 2021 Sep;15(9):2547-2560. (PMID: 33712699)
Science. 2020 Jul 31;369(6503):554-557. (PMID: 32732422)
Mol Syst Biol. 2006;2:2006.0008. (PMID: 16738554)
Proc Natl Acad Sci U S A. 1998 May 12;95(10):5752-6. (PMID: 9576956)
Cell. 2019 Jun 27;178(1):76-90.e22. (PMID: 31155236)
Genome Biol. 2006;7(7):R63. (PMID: 16859555)
J Biol Chem. 1971 Mar 25;246(6):1820-7. (PMID: 4993960)
J Bacteriol. 2014 Jun;196(11):2043-52. (PMID: 24659773)
PLoS Genet. 2019 Aug 22;15(8):e1008296. (PMID: 31437162)
J Bacteriol. 2004 Feb;186(4):1136-46. (PMID: 14762009)
Nat Biotechnol. 2024 Feb;42(2):243-246. (PMID: 37156916)
Methods Mol Biol. 2022;2548:145-167. (PMID: 36151497)
Mol Microbiol. 2008 Feb;67(4):830-48. (PMID: 18179421)
Mol Microbiol. 2012 Feb;83(3):623-39. (PMID: 22211522)
Elife. 2019 Jan 18;8:. (PMID: 30657448)
Nature. 2023 Jan;613(7945):721-728. (PMID: 36450355)
Curr Opin Chem Biol. 2015 Dec;29:79-86. (PMID: 26517565)
Nature. 2016 Apr 25;533(7602):255-9. (PMID: 27111508)
Nat Chem Biol. 2018 Jun;14(6):601-608. (PMID: 29662210)
RNA. 2003 May;9(5):566-73. (PMID: 12702815)
J Bacteriol. 2010 Jun;192(12):3132-43. (PMID: 20400549)
Sci Rep. 2020 Oct 21;10(1):17910. (PMID: 33087775)
Mol Syst Biol. 2017 May 10;13(5):931. (PMID: 28490437)
J Bacteriol. 1990 Feb;172(2):1151-4. (PMID: 1688840)
Nat Microbiol. 2023 Nov;8(11):2196-2212. (PMID: 37770760)
Antimicrob Agents Chemother. 2001 Jun;45(6):1737-42. (PMID: 11353619)
Front Microbiol. 2015 Aug 05;6:813. (PMID: 26300876)
Mol Microbiol. 2023 Jul;120(1):8-19. (PMID: 36882621)
J Bacteriol. 1994 Jan;176(1):157-65. (PMID: 7506707)
Antimicrob Agents Chemother. 2020 Dec 16;65(1):. (PMID: 33046497)
Front Microbiol. 2022 Jun 09;13:914472. (PMID: 35756023)
Nat Commun. 2025 Aug 20;16(1):7748. (PMID: 40835614)
Nat Microbiol. 2025 Jul;10(7):1698-1710. (PMID: 40528006)
J Bacteriol. 2001 May;183(10):3032-40. (PMID: 11325930)
Nature. 2018 May;557(7706):503-509. (PMID: 29769716)
Antibiotics (Basel). 2020 Jan 21;9(2):. (PMID: 31973108)
FEMS Microbiol Rev. 2008 Mar;32(2):149-67. (PMID: 18194336)
Nat Commun. 2026 Mar 02;17(1):. (PMID: 41771906)
mBio. 2023 Apr 25;14(2):e0047523. (PMID: 37017514)
Proc Natl Acad Sci U S A. 2023 May 16;120(20):e2301979120. (PMID: 37155911)
Mol Microbiol. 2026 Jun;125(6):506-545. (PMID: 41992703)
Cell Syst. 2017 Mar 22;4(3):291-305.e7. (PMID: 28189581)
PLoS Biol. 2024 Apr 29;22(4):e3002589. (PMID: 38683856)
J Bacteriol. 2009 Jan;191(1):42-51. (PMID: 18931129)
Proc Natl Acad Sci U S A. 2026 Jun 30;123(26):e2610431123. (PMID: 42335227)
Nat Microbiol. 2017 Feb 06;2:16274. (PMID: 28165460)
J Bacteriol. 2023 May 25;205(5):e0010223. (PMID: 37140386)
Nucleic Acids Res. 2014 Jan;42(Database issue):D692-8. (PMID: 24178028)
Cell. 2011 Jan 7;144(1):143-56. (PMID: 21185072)
Biosci Biotechnol Biochem. 2015;79(5):845-54. (PMID: 25559894)
Cell Syst. 2025 Nov 19;16(11):101406. (PMID: 41045937)
EMBO J. 2022 Mar 1;41(5):e109800. (PMID: 35037270)
Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4678-83. (PMID: 12682299)
PLoS One. 2012;7(4):e35698. (PMID: 22536428)
J Bacteriol. 1996 Feb;178(4):1088-93. (PMID: 8576042)
Mol Microbiol. 2017 Jan;103(2):253-268. (PMID: 27748968)
Front Microbiol. 2017 Nov 23;8:2325. (PMID: 29218041)
Nat Prod Rep. 2018 May 1;35(5):455-488. (PMID: 29799048)
Nat Rev Microbiol. 2013 Jul;11(7):435-42. (PMID: 23712350)
Mol Cell. 2013 Dec 12;52(5):643-54. (PMID: 24211265)
J Bacteriol. 1992 Jan;174(2):464-70. (PMID: 1345911)
Cell Host Microbe. 2023 Apr 12;31(4):485-499. (PMID: 37054671)
J Bacteriol. 1985 Apr;162(1):391-7. (PMID: 2858468)
Nature. 2020 Sep;585(7823):124-128. (PMID: 32848247)
Trends Microbiol. 2025 Mar;33(3):340-353. (PMID: 39613687)
Genes Dev. 1992 Jul;6(7):1165-72. (PMID: 1628824)
ISME Commun. 2024 Mar 27;4(1):ycae045. (PMID: 39081364)
Science. 2011 Feb 18;331(6019):881-6. (PMID: 21330537)
Nat Methods. 2009 Oct;6(10):767-72. (PMID: 19767758)
STAR Protoc. 2021 May 05;2(2):100521. (PMID: 34027480)
Science. 2012 Mar 2;335(6072):1103-6. (PMID: 22383849)
mBio. 2021 Oct 26;12(5):e0256121. (PMID: 34634934)
Mol Microbiol. 2021 Jul;116(1):184-199. (PMID: 33590545)
Microb Cell Fact. 2013 Jan 14;12:3. (PMID: 23311580)
Nat Commun. 2018 May 15;9(1):1912. (PMID: 29765036)
J Bacteriol. 2007 Oct;189(19):6919-27. (PMID: 17675383)
PLoS Genet. 2016 Jun 29;12(6):e1006124. (PMID: 27355376)
Mol Microbiol. 2021 Apr;115(4):739-757. (PMID: 33155333)
Nucleic Acids Res. 2025 Jan 6;53(D1):D444-D456. (PMID: 39565202)
J Bacteriol. 1999 Apr;181(8):2394-402. (PMID: 10198001)
Nat Commun. 2022 Jun 7;13(1):3165. (PMID: 35672367)
Nat Microbiol. 2024 Mar;9(3):801-813. (PMID: 38443581)
Grant Information: R35 GM118061 HHS | NIH | National Institute of General Medical Sciences (NIGMS); NA University of Queensland (UQ); NA Marcus Fund for Cell Health
Contributed Indexing: Keywords: Bacillus subtilis; chemical genomics; firmicutes
Substance Nomenclature: 0 (Bacterial Proteins)
Entry Date(s): Date Created: 20260929 Date Completed: 20260929 Latest Revision: 20261008
Update Code: 20261008
DOI: 10.1073/pnas.2619440123
PMID: 42809408
Βάση Δεδομένων: MEDLINE
FullText Text:
  Availability: 0
Header DbId: cmedm
DbLabel: MEDLINE
An: 42809408
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: The phenotypic landscape of the model firmicute Bacillus subtilis.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Kim+LM%22">Kim LM</searchLink>; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158.<br /><searchLink fieldCode="AU" term="%22Koo+BM%22">Koo BM</searchLink>; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158.<br /><searchLink fieldCode="AU" term="%22Burkhart+HN%22">Burkhart HN</searchLink>; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158.<br /><searchLink fieldCode="AU" term="%22Vollmer+D%22">Vollmer D</searchLink>; Institute for Molecular Bioscience, The University of Queensland, Brisbane 4067, QLD, Australia.<br /><searchLink fieldCode="AU" term="%22Vollmer+W%22">Vollmer W</searchLink>; Institute for Molecular Bioscience, The University of Queensland, Brisbane 4067, QLD, Australia.<br /><searchLink fieldCode="AU" term="%22Todor+H%22">Todor H</searchLink>; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158.<br /><searchLink fieldCode="AU" term="%22Gross+CA%22">Gross CA</searchLink>; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158.; Department of Cell and Tissue Biology, University of California, San Francisco, CA 94158.; Quantitative Biosciences Institute, University of California, San Francisco, CA 94158.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%227505876%22">Proceedings of the National Academy of Sciences of the United States of America</searchLink> [Proc Natl Acad Sci U S A] 2026 Oct 06; Vol. 123 (40), pp. e2619440123. <i>Date of Electronic Publication: </i>2026 Sep 29.
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22National+Academy+of+Sciences%22">National Academy of Sciences </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>7505876 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1091-6490 (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2200278424%22">00278424 </searchLink><i>NLM ISO Abbreviation: </i>Proc Natl Acad Sci U S A <i>Subsets: </i>MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Original Publication</i>: Washington, DC : National Academy of Sciences
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Bacillus+subtilis%22">Bacillus subtilis*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillus+subtilis+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillus+subtilis%22">Bacillus subtilis*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillus+subtilis+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Bacillus+subtilis%22">Bacillus subtilis*</searchLink>/<searchLink fieldCode="MM" term="%22Bacillus+subtilis+growth+%26+development%22">growth & development</searchLink><br /><searchLink fieldCode="MH" term="%22Bacterial+Proteins%22">Bacterial Proteins</searchLink>/<searchLink fieldCode="MH" term="%22Bacterial+Proteins+genetics%22">genetics</searchLink> ; <searchLink fieldCode="MH" term="%22Bacterial+Proteins%22">Bacterial Proteins</searchLink>/<searchLink fieldCode="MH" term="%22Bacterial+Proteins+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Cell+Wall%22">Cell Wall</searchLink>/<searchLink fieldCode="MH" term="%22Cell+Wall+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Phenotype%22">Phenotype</searchLink> ; <searchLink fieldCode="MH" term="%22Genome%2C+Bacterial%22">Genome, Bacterial</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Firmicutes are gram-positive bacteria with important roles in human health, disease, and industry. However, more than a quarter of genes in the model firmicute Bacillus subtilis remain completely uncharacterized, including numerous phylum-specific genes. Here, we design a compact pooled CRISPRi library targeting all protein-coding genes in B. subtilis and test its growth in ~150 stress conditions. Using data from this screen as a hypothesis generator, we perform targeted experiments, revealing that the conserved essential firmicute protein YneF is part of the SRP cotranslational protein secretion pathway. We also demonstrate that ECF-transporters play a broadly conserved role in cell wall homeostasis, perform an unbiased analysis of amino acid crossfeeding, and make additional insights into bacterial competition. This work additionally provides a rich dataset to nucleate future studies of uncharacterized genes and presents a framework for performing accessible full-genome functional genomic screens in other bacteria.
– Name: Abstract
  Label: Competing Interests
  Group: Ab
  Data: Competing interests statement:The authors declare no competing interest.
– Name: Comment
  Label: Comments
  Group: Commnt
  Data: Update of: bioRxiv. 2026 Jun 01:2026.05.13.724699. doi: 10.64898/2026.05.13.724699.. (PMID: <searchLink fieldCode="PM" term="%2242182432%22">42182432)</searchLink>
– Name: Ref
  Label: References
  Group: RefInfo
  Data: J Biol Chem. 2013 Dec 20;288(51):36385-97. (PMID: <searchLink fieldCode="PM" term="%2224151069%22">24151069)</searchLink><br />J Bacteriol. 2025 Oct 23;207(10):e0022025. (PMID: <searchLink fieldCode="PM" term="%2240919935%22">40919935)</searchLink><br />Cell. 2016 Jun 2;165(6):1493-1506. (PMID: <searchLink fieldCode="PM" term="%2227238023%22">27238023)</searchLink><br />Mol Syst Biol. 2015 Nov 17;11(11):839. (PMID: <searchLink fieldCode="PM" term="%2226577401%22">26577401)</searchLink><br />Mol Microbiol. 2014 Sep;93(6):1284-301. (PMID: <searchLink fieldCode="PM" term="%2225069588%22">25069588)</searchLink><br />Cell Syst. 2020 Nov 18;11(5):523-535.e9. (PMID: <searchLink fieldCode="PM" term="%2233080209%22">33080209)</searchLink><br />Annu Rev Biochem. 2019 Jun 20;88:551-576. (PMID: <searchLink fieldCode="PM" term="%2230485755%22">30485755)</searchLink><br />Mol Microbiol. 2006 May;60(3):765-82. (PMID: <searchLink fieldCode="PM" term="%2216629676%22">16629676)</searchLink><br />Mol Syst Biol. 2026 Apr;22(4):497-518. (PMID: <searchLink fieldCode="PM" term="%2241559189%22">41559189)</searchLink><br />Rev Med Microbiol. 2016 Jul;27(3):95-101. (PMID: <searchLink fieldCode="PM" term="%2227340340%22">27340340)</searchLink><br />J Bacteriol. 1999 Apr;181(7):2059-66. (PMID: <searchLink fieldCode="PM" term="%2210094682%22">10094682)</searchLink><br />Appl Environ Microbiol. 2024 Jan 24;90(1):e0156623. (PMID: <searchLink fieldCode="PM" term="%2238126758%22">38126758)</searchLink><br />Antimicrob Agents Chemother. 1999 May;43(5):1124-8. (PMID: <searchLink fieldCode="PM" term="%2210223924%22">10223924)</searchLink><br />J Bacteriol. 2005 Sep;187(17):5946-54. (PMID: <searchLink fieldCode="PM" term="%2216109936%22">16109936)</searchLink><br />J Cell Biol. 2003 May 26;161(4):679-84. (PMID: <searchLink fieldCode="PM" term="%2212756233%22">12756233)</searchLink><br />ISME J. 2023 Dec;17(12):2370-2380. (PMID: <searchLink fieldCode="PM" term="%2237891427%22">37891427)</searchLink><br />Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):425-30. (PMID: <searchLink fieldCode="PM" term="%2216407165%22">16407165)</searchLink><br />mBio. 2024 Nov 13;15(11):e0231024. (PMID: <searchLink fieldCode="PM" term="%2239440976%22">39440976)</searchLink><br />Biochem Biophys Res Commun. 1970 May 22;39(4):699-706. (PMID: <searchLink fieldCode="PM" term="%224992428%22">4992428)</searchLink><br />EMBO Rep. 2025 Sep;26(18):4587-4606. (PMID: <searchLink fieldCode="PM" term="%2240817173%22">40817173)</searchLink><br />Microbiology (Reading). 2002 Nov;148(Pt 11):3441-3455. (PMID: <searchLink fieldCode="PM" term="%2212427936%22">12427936)</searchLink><br />Nat Rev Microbiol. 2024 Sep;22(9):556-571. (PMID: <searchLink fieldCode="PM" term="%2238730101%22">38730101)</searchLink><br />Mol Syst Biol. 2023 Apr 12;19(4):e11544. (PMID: <searchLink fieldCode="PM" term="%2236815589%22">36815589)</searchLink><br />Nat Commun. 2024 Sep 9;15(1):7866. (PMID: <searchLink fieldCode="PM" term="%2239251624%22">39251624)</searchLink><br />Cell. 2014 Dec 4;159(6):1300-11. (PMID: <searchLink fieldCode="PM" term="%2225480295%22">25480295)</searchLink><br />Mol Syst Biol. 2024 Nov;20(11):1173-1186. (PMID: <searchLink fieldCode="PM" term="%2239349762%22">39349762)</searchLink><br />ISME J. 2021 Sep;15(9):2547-2560. (PMID: <searchLink fieldCode="PM" term="%2233712699%22">33712699)</searchLink><br />Science. 2020 Jul 31;369(6503):554-557. (PMID: <searchLink fieldCode="PM" term="%2232732422%22">32732422)</searchLink><br />Mol Syst Biol. 2006;2:2006.0008. (PMID: <searchLink fieldCode="PM" term="%2216738554%22">16738554)</searchLink><br />Proc Natl Acad Sci U S A. 1998 May 12;95(10):5752-6. (PMID: <searchLink fieldCode="PM" term="%229576956%22">9576956)</searchLink><br />Cell. 2019 Jun 27;178(1):76-90.e22. (PMID: <searchLink fieldCode="PM" term="%2231155236%22">31155236)</searchLink><br />Genome Biol. 2006;7(7):R63. (PMID: <searchLink fieldCode="PM" term="%2216859555%22">16859555)</searchLink><br />J Biol Chem. 1971 Mar 25;246(6):1820-7. (PMID: <searchLink fieldCode="PM" term="%224993960%22">4993960)</searchLink><br />J Bacteriol. 2014 Jun;196(11):2043-52. (PMID: <searchLink fieldCode="PM" term="%2224659773%22">24659773)</searchLink><br />PLoS Genet. 2019 Aug 22;15(8):e1008296. (PMID: <searchLink fieldCode="PM" term="%2231437162%22">31437162)</searchLink><br />J Bacteriol. 2004 Feb;186(4):1136-46. (PMID: <searchLink fieldCode="PM" term="%2214762009%22">14762009)</searchLink><br />Nat Biotechnol. 2024 Feb;42(2):243-246. (PMID: <searchLink fieldCode="PM" term="%2237156916%22">37156916)</searchLink><br />Methods Mol Biol. 2022;2548:145-167. (PMID: <searchLink fieldCode="PM" term="%2236151497%22">36151497)</searchLink><br />Mol Microbiol. 2008 Feb;67(4):830-48. (PMID: <searchLink fieldCode="PM" term="%2218179421%22">18179421)</searchLink><br />Mol Microbiol. 2012 Feb;83(3):623-39. (PMID: <searchLink fieldCode="PM" term="%2222211522%22">22211522)</searchLink><br />Elife. 2019 Jan 18;8:. (PMID: <searchLink fieldCode="PM" term="%2230657448%22">30657448)</searchLink><br />Nature. 2023 Jan;613(7945):721-728. (PMID: <searchLink fieldCode="PM" term="%2236450355%22">36450355)</searchLink><br />Curr Opin Chem Biol. 2015 Dec;29:79-86. (PMID: <searchLink fieldCode="PM" term="%2226517565%22">26517565)</searchLink><br />Nature. 2016 Apr 25;533(7602):255-9. (PMID: <searchLink fieldCode="PM" term="%2227111508%22">27111508)</searchLink><br />Nat Chem Biol. 2018 Jun;14(6):601-608. (PMID: <searchLink fieldCode="PM" term="%2229662210%22">29662210)</searchLink><br />RNA. 2003 May;9(5):566-73. (PMID: <searchLink fieldCode="PM" term="%2212702815%22">12702815)</searchLink><br />J Bacteriol. 2010 Jun;192(12):3132-43. (PMID: <searchLink fieldCode="PM" term="%2220400549%22">20400549)</searchLink><br />Sci Rep. 2020 Oct 21;10(1):17910. (PMID: <searchLink fieldCode="PM" term="%2233087775%22">33087775)</searchLink><br />Mol Syst Biol. 2017 May 10;13(5):931. (PMID: <searchLink fieldCode="PM" term="%2228490437%22">28490437)</searchLink><br />J Bacteriol. 1990 Feb;172(2):1151-4. (PMID: <searchLink fieldCode="PM" term="%221688840%22">1688840)</searchLink><br />Nat Microbiol. 2023 Nov;8(11):2196-2212. (PMID: <searchLink fieldCode="PM" term="%2237770760%22">37770760)</searchLink><br />Antimicrob Agents Chemother. 2001 Jun;45(6):1737-42. (PMID: <searchLink fieldCode="PM" term="%2211353619%22">11353619)</searchLink><br />Front Microbiol. 2015 Aug 05;6:813. (PMID: <searchLink fieldCode="PM" term="%2226300876%22">26300876)</searchLink><br />Mol Microbiol. 2023 Jul;120(1):8-19. (PMID: <searchLink fieldCode="PM" term="%2236882621%22">36882621)</searchLink><br />J Bacteriol. 1994 Jan;176(1):157-65. (PMID: <searchLink fieldCode="PM" term="%227506707%22">7506707)</searchLink><br />Antimicrob Agents Chemother. 2020 Dec 16;65(1):. (PMID: <searchLink fieldCode="PM" term="%2233046497%22">33046497)</searchLink><br />Front Microbiol. 2022 Jun 09;13:914472. (PMID: <searchLink fieldCode="PM" term="%2235756023%22">35756023)</searchLink><br />Nat Commun. 2025 Aug 20;16(1):7748. (PMID: <searchLink fieldCode="PM" term="%2240835614%22">40835614)</searchLink><br />Nat Microbiol. 2025 Jul;10(7):1698-1710. (PMID: <searchLink fieldCode="PM" term="%2240528006%22">40528006)</searchLink><br />J Bacteriol. 2001 May;183(10):3032-40. (PMID: <searchLink fieldCode="PM" term="%2211325930%22">11325930)</searchLink><br />Nature. 2018 May;557(7706):503-509. (PMID: <searchLink fieldCode="PM" term="%2229769716%22">29769716)</searchLink><br />Antibiotics (Basel). 2020 Jan 21;9(2):. (PMID: <searchLink fieldCode="PM" term="%2231973108%22">31973108)</searchLink><br />FEMS Microbiol Rev. 2008 Mar;32(2):149-67. (PMID: <searchLink fieldCode="PM" term="%2218194336%22">18194336)</searchLink><br />Nat Commun. 2026 Mar 02;17(1):. (PMID: <searchLink fieldCode="PM" term="%2241771906%22">41771906)</searchLink><br />mBio. 2023 Apr 25;14(2):e0047523. (PMID: <searchLink fieldCode="PM" term="%2237017514%22">37017514)</searchLink><br />Proc Natl Acad Sci U S A. 2023 May 16;120(20):e2301979120. (PMID: <searchLink fieldCode="PM" term="%2237155911%22">37155911)</searchLink><br />Mol Microbiol. 2026 Jun;125(6):506-545. (PMID: <searchLink fieldCode="PM" term="%2241992703%22">41992703)</searchLink><br />Cell Syst. 2017 Mar 22;4(3):291-305.e7. (PMID: <searchLink fieldCode="PM" term="%2228189581%22">28189581)</searchLink><br />PLoS Biol. 2024 Apr 29;22(4):e3002589. (PMID: <searchLink fieldCode="PM" term="%2238683856%22">38683856)</searchLink><br />J Bacteriol. 2009 Jan;191(1):42-51. (PMID: <searchLink fieldCode="PM" term="%2218931129%22">18931129)</searchLink><br />Proc Natl Acad Sci U S A. 2026 Jun 30;123(26):e2610431123. (PMID: <searchLink fieldCode="PM" term="%2242335227%22">42335227)</searchLink><br />Nat Microbiol. 2017 Feb 06;2:16274. (PMID: <searchLink fieldCode="PM" term="%2228165460%22">28165460)</searchLink><br />J Bacteriol. 2023 May 25;205(5):e0010223. (PMID: <searchLink fieldCode="PM" term="%2237140386%22">37140386)</searchLink><br />Nucleic Acids Res. 2014 Jan;42(Database issue):D692-8. (PMID: <searchLink fieldCode="PM" term="%2224178028%22">24178028)</searchLink><br />Cell. 2011 Jan 7;144(1):143-56. (PMID: <searchLink fieldCode="PM" term="%2221185072%22">21185072)</searchLink><br />Biosci Biotechnol Biochem. 2015;79(5):845-54. (PMID: <searchLink fieldCode="PM" term="%2225559894%22">25559894)</searchLink><br />Cell Syst. 2025 Nov 19;16(11):101406. (PMID: <searchLink fieldCode="PM" term="%2241045937%22">41045937)</searchLink><br />EMBO J. 2022 Mar 1;41(5):e109800. (PMID: <searchLink fieldCode="PM" term="%2235037270%22">35037270)</searchLink><br />Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4678-83. (PMID: <searchLink fieldCode="PM" term="%2212682299%22">12682299)</searchLink><br />PLoS One. 2012;7(4):e35698. (PMID: <searchLink fieldCode="PM" term="%2222536428%22">22536428)</searchLink><br />J Bacteriol. 1996 Feb;178(4):1088-93. (PMID: <searchLink fieldCode="PM" term="%228576042%22">8576042)</searchLink><br />Mol Microbiol. 2017 Jan;103(2):253-268. (PMID: <searchLink fieldCode="PM" term="%2227748968%22">27748968)</searchLink><br />Front Microbiol. 2017 Nov 23;8:2325. (PMID: <searchLink fieldCode="PM" term="%2229218041%22">29218041)</searchLink><br />Nat Prod Rep. 2018 May 1;35(5):455-488. (PMID: <searchLink fieldCode="PM" term="%2229799048%22">29799048)</searchLink><br />Nat Rev Microbiol. 2013 Jul;11(7):435-42. (PMID: <searchLink fieldCode="PM" term="%2223712350%22">23712350)</searchLink><br />Mol Cell. 2013 Dec 12;52(5):643-54. (PMID: <searchLink fieldCode="PM" term="%2224211265%22">24211265)</searchLink><br />J Bacteriol. 1992 Jan;174(2):464-70. (PMID: <searchLink fieldCode="PM" term="%221345911%22">1345911)</searchLink><br />Cell Host Microbe. 2023 Apr 12;31(4):485-499. (PMID: <searchLink fieldCode="PM" term="%2237054671%22">37054671)</searchLink><br />J Bacteriol. 1985 Apr;162(1):391-7. (PMID: <searchLink fieldCode="PM" term="%222858468%22">2858468)</searchLink><br />Nature. 2020 Sep;585(7823):124-128. (PMID: <searchLink fieldCode="PM" term="%2232848247%22">32848247)</searchLink><br />Trends Microbiol. 2025 Mar;33(3):340-353. (PMID: <searchLink fieldCode="PM" term="%2239613687%22">39613687)</searchLink><br />Genes Dev. 1992 Jul;6(7):1165-72. (PMID: <searchLink fieldCode="PM" term="%221628824%22">1628824)</searchLink><br />ISME Commun. 2024 Mar 27;4(1):ycae045. (PMID: <searchLink fieldCode="PM" term="%2239081364%22">39081364)</searchLink><br />Science. 2011 Feb 18;331(6019):881-6. (PMID: <searchLink fieldCode="PM" term="%2221330537%22">21330537)</searchLink><br />Nat Methods. 2009 Oct;6(10):767-72. (PMID: <searchLink fieldCode="PM" term="%2219767758%22">19767758)</searchLink><br />STAR Protoc. 2021 May 05;2(2):100521. (PMID: <searchLink fieldCode="PM" term="%2234027480%22">34027480)</searchLink><br />Science. 2012 Mar 2;335(6072):1103-6. (PMID: <searchLink fieldCode="PM" term="%2222383849%22">22383849)</searchLink><br />mBio. 2021 Oct 26;12(5):e0256121. (PMID: <searchLink fieldCode="PM" term="%2234634934%22">34634934)</searchLink><br />Mol Microbiol. 2021 Jul;116(1):184-199. (PMID: <searchLink fieldCode="PM" term="%2233590545%22">33590545)</searchLink><br />Microb Cell Fact. 2013 Jan 14;12:3. (PMID: <searchLink fieldCode="PM" term="%2223311580%22">23311580)</searchLink><br />Nat Commun. 2018 May 15;9(1):1912. (PMID: <searchLink fieldCode="PM" term="%2229765036%22">29765036)</searchLink><br />J Bacteriol. 2007 Oct;189(19):6919-27. (PMID: <searchLink fieldCode="PM" term="%2217675383%22">17675383)</searchLink><br />PLoS Genet. 2016 Jun 29;12(6):e1006124. (PMID: <searchLink fieldCode="PM" term="%2227355376%22">27355376)</searchLink><br />Mol Microbiol. 2021 Apr;115(4):739-757. (PMID: <searchLink fieldCode="PM" term="%2233155333%22">33155333)</searchLink><br />Nucleic Acids Res. 2025 Jan 6;53(D1):D444-D456. (PMID: <searchLink fieldCode="PM" term="%2239565202%22">39565202)</searchLink><br />J Bacteriol. 1999 Apr;181(8):2394-402. (PMID: <searchLink fieldCode="PM" term="%2210198001%22">10198001)</searchLink><br />Nat Commun. 2022 Jun 7;13(1):3165. (PMID: <searchLink fieldCode="PM" term="%2235672367%22">35672367)</searchLink><br />Nat Microbiol. 2024 Mar;9(3):801-813. (PMID: <searchLink fieldCode="PM" term="%2238443581%22">38443581)</searchLink>
– Name: GrantInfo
  Label: Grant Information
  Group: Grant
  Data: R35 GM118061 HHS | NIH | National Institute of General Medical Sciences (NIGMS); NA University of Queensland (UQ); NA Marcus Fund for Cell Health
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>Bacillus subtilis; chemical genomics; firmicutes
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 0 (Bacterial Proteins)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: <i>Date Created: </i>20260929 <i>Date Completed: </i>20260929 <i>Latest Revision: </i>20261008
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20261008
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1073/pnas.2619440123
– Name: AN
  Label: PMID
  Group: ID
  Data: 42809408
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42809408
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1073/pnas.2619440123
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: e2619440123
    Subjects:
      – SubjectFull: Bacterial Proteins genetics
        Type: general
      – SubjectFull: Bacterial Proteins metabolism
        Type: general
      – SubjectFull: Cell Wall metabolism
        Type: general
      – SubjectFull: Phenotype
        Type: general
      – SubjectFull: Genome, Bacterial
        Type: general
      – SubjectFull: Bacillus subtilis genetics
        Type: general
      – SubjectFull: Bacillus subtilis metabolism
        Type: general
      – SubjectFull: Bacillus subtilis growth & development
        Type: general
    Titles:
      – TitleFull: The phenotypic landscape of the model firmicute Bacillus subtilis.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Kim LM
      – PersonEntity:
          Name:
            NameFull: Koo BM
      – PersonEntity:
          Name:
            NameFull: Burkhart HN
      – PersonEntity:
          Name:
            NameFull: Vollmer D
      – PersonEntity:
          Name:
            NameFull: Vollmer W
      – PersonEntity:
          Name:
            NameFull: Todor H
      – PersonEntity:
          Name:
            NameFull: Gross CA
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 06
              M: 10
              Text: 2026 Oct 06
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 1091-6490
          Numbering:
            – Type: volume
              Value: 123
            – Type: issue
              Value: 40
          Titles:
            – TitleFull: Proceedings of the National Academy of Sciences of the United States of America
              Type: main
ResultId 1