Academic Journal
The phenotypic landscape of the model firmicute Bacillus subtilis.
| Τίτλος: | The phenotypic landscape of the model firmicute Bacillus subtilis. |
|---|---|
| Συγγραφείς: | Kim LM; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158., Koo BM; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158., Burkhart HN; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158., Vollmer D; Institute for Molecular Bioscience, The University of Queensland, Brisbane 4067, QLD, Australia., Vollmer W; Institute for Molecular Bioscience, The University of Queensland, Brisbane 4067, QLD, Australia., Todor H; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158., Gross CA; Department of Microbiology and Immunology, University of California, San Francisco, CA 94158.; Department of Cell and Tissue Biology, University of California, San Francisco, CA 94158.; Quantitative Biosciences Institute, University of California, San Francisco, CA 94158. |
| Πηγή: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2026 Oct 06; Vol. 123 (40), pp. e2619440123. Date of Electronic Publication: 2026 Sep 29. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : National Academy of Sciences |
| Ιατρικοί όροι (MeSH): | Bacillus subtilis*/genetics , Bacillus subtilis*/metabolism , Bacillus subtilis*/growth & development, Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cell Wall/metabolism ; Phenotype ; Genome, Bacterial |
| Περίληψη: | Firmicutes are gram-positive bacteria with important roles in human health, disease, and industry. However, more than a quarter of genes in the model firmicute Bacillus subtilis remain completely uncharacterized, including numerous phylum-specific genes. Here, we design a compact pooled CRISPRi library targeting all protein-coding genes in B. subtilis and test its growth in ~150 stress conditions. Using data from this screen as a hypothesis generator, we perform targeted experiments, revealing that the conserved essential firmicute protein YneF is part of the SRP cotranslational protein secretion pathway. We also demonstrate that ECF-transporters play a broadly conserved role in cell wall homeostasis, perform an unbiased analysis of amino acid crossfeeding, and make additional insights into bacterial competition. This work additionally provides a rich dataset to nucleate future studies of uncharacterized genes and presents a framework for performing accessible full-genome functional genomic screens in other bacteria. |
| Competing Interests: | Competing interests statement:The authors declare no competing interest. |
| Σχόλια: | Update of: bioRxiv. 2026 Jun 01:2026.05.13.724699. doi: 10.64898/2026.05.13.724699.. (PMID: 42182432) |
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| Grant Information: | R35 GM118061 HHS | NIH | National Institute of General Medical Sciences (NIGMS); NA University of Queensland (UQ); NA Marcus Fund for Cell Health |
| Contributed Indexing: | Keywords: Bacillus subtilis; chemical genomics; firmicutes |
| Substance Nomenclature: | 0 (Bacterial Proteins) |
| Entry Date(s): | Date Created: 20260929 Date Completed: 20260929 Latest Revision: 20261008 |
| Update Code: | 20261008 |
| DOI: | 10.1073/pnas.2619440123 |
| PMID: | 42809408 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1091-6490 |
|---|---|
| DOI: | 10.1073/pnas.2619440123 |