Academic Journal
Periodontal Disease Diagnosis by a Chemically Etched Single-Mode Fiber-Optic Biosensor for Label-Free Detection of Matrix Metalloproteinase-8 (MMP-8).
| Τίτλος: | Periodontal Disease Diagnosis by a Chemically Etched Single-Mode Fiber-Optic Biosensor for Label-Free Detection of Matrix Metalloproteinase-8 (MMP-8). |
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| Συγγραφείς: | Tovar R Jr; Department of Bioengineering, Civil Engineering, and Environmental Engineering, U. A. Whitaker College of Engineering, Florida Gulf Coast University, 10501 FGCU Boulevard South, Fort Myers, FL 33965, USA., Sozkes S; Department of Bioengineering, Civil Engineering, and Environmental Engineering, U. A. Whitaker College of Engineering, Florida Gulf Coast University, 10501 FGCU Boulevard South, Fort Myers, FL 33965, USA.; Department of Biomaterials, Biomedical Engineering Corlu Faculty, Tekirdag Namik Kemal University, Yılmaz Alpaslan Cd. No:25, 59850 Tekirdag, Turkey., Sypabekova M; Department of Bioengineering, Civil Engineering, and Environmental Engineering, U. A. Whitaker College of Engineering, Florida Gulf Coast University, 10501 FGCU Boulevard South, Fort Myers, FL 33965, USA. |
| Πηγή: | Biosensors [Biosensors (Basel)] 2026 Sep 03; Vol. 16 (9). Date of Electronic Publication: 2026 Sep 03. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: MDPI Pub Country of Publication: Switzerland NLM ID: 101609191 Publication Model: Electronic Cited Medium: Internet ISSN: 2079-6374 (Electronic) Linking ISSN: 20796374 NLM ISO Abbreviation: Biosensors (Basel) Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI Pub. |
| Ιατρικοί όροι (MeSH): | Matrix Metalloproteinase 8*/analysis , Periodontal Diseases*/diagnosis , Biosensing Techniques* , Fiber Optic Technology*, Humans ; Optical Fibers ; Saliva ; Limit of Detection ; Biomarkers |
| Περίληψη: | A miniature label-free biosensor based on a chemically etched single-mode optical fiber (SMF) is reported for the detection of matrix metalloproteinase-8 (MMP-8), a salivary biomarker of active periodontitis with a clinical decision threshold of 20 ng/mL. Fibers etched in 48% hydrofluoric acid to a waist diameter of 16.0 ± 1.4 µm gave a mean refractive index (RI) sensitivity of 376.8%/RIU and an RI limit of detection (LOD) of 5.9 × 10-4 RIU. Fibers were tested with MMP-8 spiked into phosphate-buffered saline (PBS) and into saliva over 0-200 ng/mL using a post-rinse protocol with per-fiber matrix subtraction. Dose-responses followed a Langmuir isotherm (Kd = 7.1 ng/mL in PBS, 12.7 ng/mL in saliva), with cohort LODs of 0.043 and 0.52 ng/mL, both well below the threshold. MMP-9 (100 ng/mL) and human serum albumin (1 mg/mL) gave negligible responses (≤2.7%, versus 68.2% for MMP-8 at 100 ng/mL); antibody immobilization was confirmed by confocal immunofluorescence. A commercial sandwich ELISA on the same spike series gave a matched-matrix LOD of 41.1 ng/mL, nearly two orders of magnitude higher. This performance requires no metal coating, nanostructuring, label, or signal amplification, only a single wet-etching step on stock telecommunications fiber. |
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| Grant Information: | Head Start Grant from the U. A. Whitaker College of Engineering, Florida Gulf Coast University, 2025-2026. |
| Contributed Indexing: | Keywords: MMP-8; etched single-mode fiber; evanescent wave; label-free detection; optical fiber biosensor; periodontal disease; point-of-care diagnostics; spiked saliva |
| Substance Nomenclature: | EC 3.4.24.34 (Matrix Metalloproteinase 8) 0 (Biomarkers) |
| Entry Date(s): | Date Created: 20260924 Date Completed: 20260924 Latest Revision: 20260926 |
| Update Code: | 20260926 |
| PubMed Central ID: | PMC13604195 |
| DOI: | 10.3390/bios16090491 |
| PMID: | 42783158 |
| Βάση Δεδομένων: | MEDLINE |
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