Academic Journal
Neurofilament transport kinetics can be explained by the activity of small numbers of independent kinesin and dynein motors.
| Τίτλος: | Neurofilament transport kinetics can be explained by the activity of small numbers of independent kinesin and dynein motors. |
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| Συγγραφείς: | Nowier RM; Department of Physics and Astronomy and Quantitative Biology Institute, Ohio University, Athens, OH, USA., Johnson CM; Department of Physics and Astronomy and Quantitative Biology Institute, Ohio University, Athens, OH, USA., Fenn JD; Department of Neuroscience, Ohio State University, Columbus, OH, USA., Brown A; Department of Neuroscience, Ohio State University, Columbus, OH, USA. Electronic address: brown.2302@osu.edu., Jung P; Department of Physics and Astronomy and Quantitative Biology Institute, Ohio University, Athens, OH, USA. Electronic address: jungp@ohio.edu. |
| Πηγή: | Biophysical journal [Biophys J] 2026 Oct 20; Vol. 125 (20), pp. 5937-5957. Date of Electronic Publication: 2026 Sep 17. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Cell Press Country of Publication: United States NLM ID: 0370626 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1542-0086 (Electronic) Linking ISSN: 00063495 NLM ISO Abbreviation: Biophys J Subsets: MEDLINE |
| Imprint Name(s): | Publication: Cambridge, MA : Cell Press Original Publication: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society. |
| Ιατρικοί όροι (MeSH): | Dyneins*/metabolism , Kinesins*/metabolism , Intermediate Filaments*/metabolism, Kinetics ; Animals ; Models, Biological ; Stochastic Processes |
| Περίληψη: | Neurofilaments, which are intermediate filaments, are long flexible protein polymers that are transported bidirectionally along microtubule tracks in axons. Kymograph analysis of neurofilaments has revealed rapid intermittent movement with average run lengths consistent with the processive bouts of single motors, and frequent pauses and reversals that suggest rapid switching between opposing motors. To explore this, we used discrete stochastic models of kinesin and dynein motors to simulate neurofilament movement by teams of independent motors in axons. We describe an unbiased automated sampling algorithm to identify the numbers of kinesin and dynein motors required to match the range of pause times, run times, run lengths, and velocities for neurofilaments in a large data set of ∼37,000 runs and pauses acquired with 30.3 ms resolution. We find that the diverse kinetic behavior of neurofilaments can be explained by the activity of 1-2 kinesin or dynein motors, which interact indirectly via the mechanical forces they exert on the neurofilament cargo. Simultaneous engagement of opposing motors was rare in our simulations, indicating that the motile behavior can be explained by alternating kinesin and dynein activity without a competition between motors of opposite directionality. (Copyright © 2026 Biophysical Society. Published by Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of interests The authors declare no competing interests. |
| Contributed Indexing: | Keywords: axon; axonal transport; dynein; kinesin; neurofilament |
| Substance Nomenclature: | EC 3.6.4.2 (Dyneins) EC 3.6.4.4 (Kinesins) |
| Entry Date(s): | Date Created: 20260917 Date Completed: 20261020 Latest Revision: 20261020 |
| Update Code: | 20261021 |
| DOI: | 10.1016/j.bpj.2026.09.016 |
| PMID: | 42750279 |
| Βάση Δεδομένων: | MEDLINE |
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