Academic Journal
Dnmt3b and Dnmt3l knockdown reduces blastocyst development in early mouse embryos.
| Τίτλος: | Dnmt3b and Dnmt3l knockdown reduces blastocyst development in early mouse embryos. |
|---|---|
| Συγγραφείς: | Bozdemir N; Department of Histology and Embryology, Ankara Medipol University School of Medicine, Altindag, 06050, Ankara, Turkey., Cinar O; Department of Histology and Embryology, Ankara University School of Medicine, Altindag, 06080, Ankara, Turkey., Oznacar T; Department of Biostatistics, Ankara Medipol University School of Medicine, Altindag, 06050, Ankara, Turkey., Ceylan AC; Department of Medical Genetics, Ankara Yıldırım Beyazit University School of Medicine, Cankaya, 06800, Ankara, Turkey., Cinar FU; Department of Histology and Embryology, Ankara Medipol University School of Medicine, Altindag, 06050, Ankara, Turkey. fatma.uysal@ankaramedipol.edu.tr. |
| Πηγή: | Histochemistry and cell biology [Histochem Cell Biol] 2026 Sep 16; Vol. 164 (1). Date of Electronic Publication: 2026 Sep 16. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer Country of Publication: Germany NLM ID: 9506663 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-119X (Electronic) Linking ISSN: 09486143 NLM ISO Abbreviation: Histochem Cell Biol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Berlin : Springer |
| Ιατρικοί όροι (MeSH): | DNA (Cytosine-5-)-Methyltransferases*/genetics , DNA (Cytosine-5-)-Methyltransferases*/deficiency , DNA (Cytosine-5-)-Methyltransferases*/metabolism , Blastocyst*/metabolism , Blastocyst*/cytology , Embryonic Development*/genetics , Gene Knockdown Techniques*, RNA, Small Interfering/genetics ; Animals ; DNA Methyltransferase 3B ; Mice ; Female ; DNA Methylation |
| Περίληψη: | A one-cell embryo called a zygote develops into a blastocyst through several successive cell divisions and lineage specification, this process is called early embryo development. Both embryonic genome activation (EGA) and the first lineage specification during early embryonic development depend on tightly coordinated epigenomic organization. Regulation of the epigenome is primarily governed by DNA methylation mediated through DNA methyltransferase (Dnmt) enzymes. Dnmt1 is responsible for the maintenance of methylation during cellular division, while Dnmt3a/Dnmt3b enzymes play a role in the establishment of de novo methylation particularly during gametogenesis and early embryo development. Despite its lack of catalytic activity, Dnmt3l functions as a cofactor enhancing Dnmt3a/3b activity. Dnmt3b deficiency results in global hypomethylation and ultimately embryonic lethality. In this study, we aim to elucidate the effect of Dnmt3b and Dnmt3l silencing on early embryo development. For this purpose, our experimental groups were established using an in vitro mouse embryo development model: control, Dnmt3b small interfering RNA (siRNA), Dnmt3l siRNA, and a nontargeting siRNA group. Following gene silencing at the one-cell stage, embryonic developmental competence, the expression pattern of nonsilenced Dnmt enzymes, global DNA methylation levels, and transcriptome profiles were analyzed at the blastocyst stage. Dnmt3b/3l silencing resulted in decreased global DNA methylation and Dnmt1/3a expression, and reduced blastocyst rate. Differentially expressed genes included those involved in X-chromosome inactivation (Xist), transcriptional regulation (Rn7sk), translation (Eef1a1, Eef2), trophoblast development (Hsd3b1), compaction (Gja1), and oxidative phosphorylation (CYTB, COX1, mt-Rnr1). Our findings indicate that siRNA-mediated knockdown of Dnmt3b and Dnmt3l is associated with reduced blastocyst development, impaired embryo quality, and alterations in DNA methylation-related processes during early embryonic development. (© 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
| Competing Interests: | Declarations. Conflict of interest: The authors declare no conflicts interests. Ethical approval: The experimental protocol was approved by the Animal Care and Usage Committee of Ankara University (protocol no. 2023-6-44). |
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| Grant Information: | 123S031. Türkiye Bilimsel ve Teknolojik Araştırma Kurumu |
| Contributed Indexing: | Keywords: Blastocyst; DNA methylation; Dnmt3b; Dnmt3l; Early embryo development |
| Substance Nomenclature: | EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases) 0 (DNA Methyltransferase 3B) EC 2.1.1.- (Dnmt3l protein, mouse) 0 (RNA, Small Interfering) |
| Entry Date(s): | Date Created: 20260916 Date Completed: 20260917 Latest Revision: 20261008 |
| Update Code: | 20261008 |
| DOI: | 10.1007/s00418-026-02540-3 |
| PMID: | 42749899 |
| Βάση Δεδομένων: | MEDLINE |
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