Academic Journal
Automated sample preparation microfluidic platform for immuno-LAMP detection: application to troponin I quantification.
| Τίτλος: | Automated sample preparation microfluidic platform for immuno-LAMP detection: application to troponin I quantification. |
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| Συγγραφείς: | Tardieux V; Univ. Grenoble Alpes, CEA-Leti, 38000 Grenoble, France. myriam.cubizolles@cea.fr., Aubret M; Univ. Grenoble Alpes, CEA-Leti, 38000 Grenoble, France. myriam.cubizolles@cea.fr., Parent C; Univ. Grenoble Alpes, CEA-Leti, 38000 Grenoble, France. myriam.cubizolles@cea.fr., Fouillet Y; Univ. Grenoble Alpes, CEA-Leti, 38000 Grenoble, France. myriam.cubizolles@cea.fr., Laurent P; Univ. Grenoble Alpes, CEA-Leti, 38000 Grenoble, France. myriam.cubizolles@cea.fr., Saint-Pierre C; Univ. Grenoble Alpes, CEA, CNRS, Grenoble INP, IRIG, SyMMES/CREAB, 38000 Grenoble, France., Boizot F; Univ. Grenoble Alpes, CEA-Leti, 38000 Grenoble, France. myriam.cubizolles@cea.fr., Gherbi I; Univ. Grenoble Alpes, CEA-Leti, 38000 Grenoble, France. myriam.cubizolles@cea.fr., Gudimard L; Univ. Grenoble Alpes, CEA-Leti, 38000 Grenoble, France. myriam.cubizolles@cea.fr., Buhot A; Univ. Grenoble Alpes, CEA, CNRS, Grenoble INP, IRIG, SyMMES/CREAB, 38000 Grenoble, France., Cubizolles M; Univ. Grenoble Alpes, CEA-Leti, 38000 Grenoble, France. myriam.cubizolles@cea.fr. |
| Πηγή: | Analytical methods : advancing methods and applications [Anal Methods] 2026 Oct 15; Vol. 18 (39), pp. 8876-8887. Date of Electronic Publication: 2026 Oct 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: RSC Pub Country of Publication: England NLM ID: 101519733 Publication Model: Electronic Cited Medium: Internet ISSN: 1759-9679 (Electronic) Linking ISSN: 17599660 NLM ISO Abbreviation: Anal Methods Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Cambridge : RSC Pub., 2009- |
| Ιατρικοί όροι (MeSH): | Troponin I*/blood , Nucleic Acid Amplification Techniques*/methods , Nucleic Acid Amplification Techniques*/instrumentation , Microfluidic Analytical Techniques*/instrumentation , Microfluidic Analytical Techniques*/methods , Molecular Diagnostic Techniques*/methods , Molecular Diagnostic Techniques*/instrumentation, Immunoassay/methods ; Immunoassay/instrumentation ; Humans ; Automation |
| Περίληψη: | Easy-to-use, highly-sensitive, and quantitative assays over a large dynamic range are needed to improve patient care and disease monitoring. These factors are challenging to address simultaneously using classical methods. The laboratory ELISA assay is relevant for complex matrices over a linear quantification range, whereas immunoassay implementation in commercial lateral flow assays (LFA) remains mostly qualitative. In the case of myocardial infarction (MI), the cardiac biomarker troponin I (cTnI) is present at low levels in the blood of a healthy patient but increases by several orders of magnitude at the onset of symptoms. Here, we aim to detect cTnI as a proof of concept for developing a sample preparation microfluidic system for the immuno loop-mediated isothermal amplification (immuno-LAMP) method. Immuno-LAMP combines the sensitivity and selectivity of antibody recognition with the wide dynamic range allowed through nucleic acid amplifications. cTnI immuno-LAMP assays are firstly validated in a well-plate, and then optimized and integrated into microfluidic cartridges to improve reliability and enable automation. A network of valves and chambers controls a sample preparation module that handles magnetic beads coupled to capture antibodies. Detection antibodies coupled to short oligonucleotides, used to form the immuno-complex, hybridize with an oligonucleotide structure called a dumbbell. This structure is isothermally amplified in tubes to allow the quantification of cTnI. Our work has demonstrated the ability of our automated sample preparation platform to quantify cTnI spiked in human blood plasma at sub-picomolar concentrations. This prototype opens perspectives for integrating the whole immuno-LAMP method from human plasma samples in a microfluidic device including the LAMP amplification and detection. |
| Substance Nomenclature: | 0 (Troponin I) |
| SCR Protocol: | LAMP assay |
| Entry Date(s): | Date Created: 20260914 Date Completed: 20261015 Latest Revision: 20261015 |
| Update Code: | 20261016 |
| DOI: | 10.1039/d6ay00864j |
| PMID: | 42734110 |
| Βάση Δεδομένων: | MEDLINE |
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