Academic Journal
NIE Antigen Mapping for Serological Diagnostic of Strongyloidiasis.
| Τίτλος: | NIE Antigen Mapping for Serological Diagnostic of Strongyloidiasis. |
|---|---|
| Συγγραφείς: | Cambra-Pellejà M; ISGlobal, 08036Barcelona, Spain.; GraphenicaLab SL, 08018Barcelona, Spain.; Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, 08007Barcelona, Spain., Aguilar R; ISGlobal, 08036Barcelona, Spain., Vidal M; ISGlobal, 08036Barcelona, Spain., Pejenaute A; Center for Cooperative Research in Biosciences CIC bioGUNE, Basque Research and Technology Alliance (BRTA), Bizkaia Science and Technology Park, Building 800, 48160Derio, Spain.; Tekniker, Basque Research and Technology Alliance (BRTA), C/Iñaki Goenaga 5, 20600Eibar, Spain., Jiménez A; ISGlobal, 08036Barcelona, Spain., Fleitas P; ISGlobal, 08036Barcelona, Spain., Simao CD; GraphenicaLab SL, 08018Barcelona, Spain., Baptista-Pires L; I3N, Physics Department, University of Aveiro, Aveiro3810-193, Portugal., Camprubí-Ferrer D; ISGlobal, 08036Barcelona, Spain.; Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, 08007Barcelona, Spain.; International Health Department Hospital Clinic de Barcelona, 08036Barcelona, Spain.; CIBER de Enfermedades Infecciosas (CIBERINFEC), Barcelona, Spain., Muñoz J; ISGlobal, 08036Barcelona, Spain.; Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, 08007Barcelona, Spain.; International Health Department Hospital Clinic de Barcelona, 08036Barcelona, Spain.; CIBER de Enfermedades Infecciosas (CIBERINFEC), Barcelona, Spain., Ortega-Quintanilla G; Center for Cooperative Research in Biosciences CIC bioGUNE, Basque Research and Technology Alliance (BRTA), Bizkaia Science and Technology Park, Building 800, 48160Derio, Spain.; Ikerbasque, Basque Foundation for Science, Plaza Euskadi 5, 48009Bilbao, Spain., Dobaño C; ISGlobal, 08036Barcelona, Spain.; CIBER de Enfermedades Infecciosas (CIBERINFEC), Barcelona, Spain., Parolo C; ISGlobal, 08036Barcelona, Spain.; INTERFIBIO Research Group, Departament d'Enginyeria Química, Universitat Rovira iVirgili, 43007Tarragona, Spain., Gandasegui J; ISGlobal, 08036Barcelona, Spain. |
| Πηγή: | ACS infectious diseases [ACS Infect Dis] 2026 Sep 11; Vol. 12 (9), pp. 3104-3113. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: ACS Publications Country of Publication: United States NLM ID: 101654580 Publication Model: Print Cited Medium: Internet ISSN: 2373-8227 (Electronic) Linking ISSN: 23738227 NLM ISO Abbreviation: ACS Infect Dis Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : ACS Publications, [2015]- |
| Ιατρικοί όροι (MeSH): | Strongyloidiasis*/diagnosis , Strongyloidiasis*/immunology , Epitope Mapping*/methods , Antigens, Helminth*/immunology , Antigens, Helminth*/chemistry , Antigens, Helminth*/genetics , Strongyloides stercoralis*/immunology, Serologic Tests/methods ; Antibodies, Helminth/blood ; Immunoglobulin G/blood ; Animals ; Humans ; Amino Acid Sequence |
| Περίληψη: | Strongyloidiasis is a neglected tropical disease for which scalable serological diagnostics are essential to support control programmes. The Strongyloides stercoralis NIE antigen is widely used for antibody detection, but its antigenic determinants remain incompletely defined. Here, we performed epitope mapping of the NIE antigen using a panel of 23 overlapping 15-mer peptides and 4 bioinformatically predicted peptides. Immunoreactivity was evaluated by detecting IgG assay in sera from infected and noninfected individuals. Full-length NIE showed excellent diagnostic performance (AUC = 0.934), whereas four peptides (11, 12, 17, 18) demonstrated moderate discrimination (AUC = 0.59-0.64). Combining peptides improved performance (AUC > 0.70), with peptides 12 and 18 consistently contributing to the best models. Structural mapping revealed two solvent-exposed regions corresponding to peptide pairs 11-12 and 17-18. These findings identify key immunoreactive regions within NIE and provide a structural basis for antigen engineering, while confirming the superior performance of the full-length antigen for serodiagnosis. (© 2026 The Authors. Published by American Chemical Society.) |
| Grant Information: | NA ?Ayudas a proyectos de colaboraci?n p?blico-privada? (CPP2022-009773), MCIN/AEI/10?13039/501100011033, ?NextGenerationEU?/PRTR; NA ?Ayudas para la formaci?n de doctores en empresas ?Doctorados Industriales?? (DIN2022-012602), MCIN/AEI/10?13039/501100011033; NA CEX2023-0001290-S, MCIN/AEI/10.13039/501100011033, Generalitat de Catalunya; NA FCT I.P. for the research action; NA i3N, (UIDB/ 50025/2020-2023 & UIDP/50025/2020-2023 & LA/P/0037/2020), FCT/MEC; NA Pla de Doctorats Industrials del Departament de Recerca i Universitats de la Generalitat de Catalunya; NA Ram?n y Cajal grant, Spanish Ministry of Science |
| Contributed Indexing: | Keywords: NIE antigen; Strongyloidiasis; diagnostic; epitope mapping; serology |
| Substance Nomenclature: | 0 (Antigens, Helminth) 0 (Antibodies, Helminth) 0 (Immunoglobulin G) |
| Entry Date(s): | Date Created: 20260911 Date Completed: 20260911 Latest Revision: 20260911 |
| Update Code: | 20260912 |
| DOI: | 10.1021/acsinfecdis.6c00271 |
| PMID: | 42725987 |
| Βάση Δεδομένων: | MEDLINE |
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