Academic Journal
Transcriptional dynamics of the murine heart during perinatal development at single-cell resolution.
| Τίτλος: | Transcriptional dynamics of the murine heart during perinatal development at single-cell resolution. |
|---|---|
| Συγγραφείς: | Feulner L; Cardiovascular Genetics, Department of Paediatrics, Centre Hospitalier Universitaire Sainte-Justine Research Centre, Montreal, QC H3T 1C5, Canada.; University of Montreal, Department of Molecular Biology, Montreal, QC H3T 1J4, Canada., Wünnemann F; Cardiovascular Genetics, Department of Paediatrics, Centre Hospitalier Universitaire Sainte-Justine Research Centre, Montreal, QC H3T 1C5, Canada.; Institute for Computational Biomedicine, Faculty of Medicine, University Hospital Heidelberg and Heidelberg University, 69120 Heidelberg, Germany., Rafatian N; Cardiovascular Genetics, Department of Paediatrics, Centre Hospitalier Universitaire Sainte-Justine Research Centre, Montreal, QC H3T 1C5, Canada., Liang J; Cardiovascular Genetics, Department of Paediatrics, Centre Hospitalier Universitaire Sainte-Justine Research Centre, Montreal, QC H3T 1C5, Canada.; McGill University, Department of Anatomy and Cell Biology, Montreal, QC H3A 0G4, Canada., Medouni A; Cardiovascular Genetics, Department of Paediatrics, Centre Hospitalier Universitaire Sainte-Justine Research Centre, Montreal, QC H3T 1C5, Canada.; McGill University, Department of Anatomy and Cell Biology, Montreal, QC H3A 0G4, Canada., Hofmann P; German Centre for Cardiovascular Research, Institute of Medical Genetics, Carl von Ossietzky University, 26129 Oldenburg, Germany., Hitz MP; German Centre for Cardiovascular Research, Institute of Medical Genetics, Carl von Ossietzky University, 26129 Oldenburg, Germany., Schapiro D; Institute for Computational Biomedicine, Faculty of Medicine, University Hospital Heidelberg and Heidelberg University, 69120 Heidelberg, Germany., Leclerc S; Cardiovascular Genetics, Department of Paediatrics, Centre Hospitalier Universitaire Sainte-Justine Research Centre, Montreal, QC H3T 1C5, Canada.; Centre Hospitalier de I'Université de Montreal (CHUM) Research Centre, OPTILAB Diagnostic Laboratory, Montreal, QC H2X 0C1, Canada., van Vliet PP; Cardiovascular Genetics, Department of Paediatrics, Centre Hospitalier Universitaire Sainte-Justine Research Centre, Montreal, QC H3T 1C5, Canada., Andelfinger G; Cardiovascular Genetics, Department of Paediatrics, Centre Hospitalier Universitaire Sainte-Justine Research Centre, Montreal, QC H3T 1C5, Canada. |
| Πηγή: | Biology open [Biol Open] 2026 Sep 15; Vol. 15 (9). Date of Electronic Publication: 2026 Sep 11. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Company of Biologists Ltd Country of Publication: England NLM ID: 101578018 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2046-6390 (Electronic) Linking ISSN: 20466390 NLM ISO Abbreviation: Biol Open Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Cambridge : Company of Biologists Ltd. |
| Ιατρικοί όροι (MeSH): | Single-Cell Analysis*/methods , Heart*/embryology , Heart*/growth & development , Myocardium*/metabolism , Gene Expression Regulation, Developmental* , Transcription, Genetic*, RNA, Long Noncoding/genetics ; Endothelial Cells/metabolism ; Induced Pluripotent Stem Cells/cytology ; Induced Pluripotent Stem Cells/metabolism ; Computational Biology/methods ; Animals ; Mice ; Humans ; Single-Cell Gene Expression Analysis ; Gene Expression Profiling ; Transcriptome |
| Περίληψη: | Heart maturation and remodelling during the foetal and early postnatal period are critical for the proper survival and growth of the foetus, yet our knowledge of the molecular processes involved is lacking for many cardiac cell types. To gain a deeper understanding of the transcriptional dynamics of the heart during the perinatal period, we performed single-cell RNA sequencing on foetal and early postnatal mouse hearts to establish a catalogue of 49,769 single-cell transcriptomes and used this for bioinformatics analyses. Pseudotime analyses and RNAscope fluorescence in situ hybridisation showed that while lncRNA H19 expression decreased over time in multiple cardiac cell types, it remained stably expressed in endocardial and valve endothelial cells. To further investigate this in a human setting, we generated human induced pluripotent stem cell (hiPSC)-derived endothelial cells and used single-cell data to develop a sorting strategy to separate endocardial from vascular endothelial cells, ensuring endothelial subtype purity. Knockdown of H19 in hiPSC-derived endocardial cells resulted in morphological changes, decreased expression of endothelial markers, and increased expression of mesenchymal markers, consistent with the onset of endothelial-to-mesenchymal transition. Together, our data suggest a role for H19 in maintaining endocardial and valve endothelial cell states during perinatal heart maturation. (© 2026. Published by The Company of Biologists.) |
| Competing Interests: | Competing interests The authors declare no competing or financial interests. |
| References: | Can J Physiol Pharmacol. 1991 Oct;69(10):1486-92. (PMID: 1723347) Cell Rep. 2015 Nov 24;13(8):1705-16. (PMID: 26586429) Sci Rep. 2018 Oct 18;8(1):15362. (PMID: 30337648) J Am Heart Assoc. 2018 Oct 16;7(20):e010378. (PMID: 30371266) Circ Res. 2011 Jul 8;109(2):183-92. (PMID: 21597012) Nature. 2018 Feb 22;554(7693):475-480. (PMID: 29443965) Anat Rec (Hoboken). 2014 Aug;297(8):1414-29. (PMID: 24863187) Circ Res. 2020 Oct 9;127(9):e232-e249. (PMID: 32811295) Cell. 2019 Dec 12;179(7):1647-1660.e19. (PMID: 31835037) Diabetologia. 2019 Mar;62(3):517-530. (PMID: 30612136) Front Physiol. 2018 Jul 19;9:959. (PMID: 30072919) Stem Cell Reports. 2020 May 12;14(5):770-787. (PMID: 32359445) Cell Biosci. 2016 Nov 22;6:58. (PMID: 27895893) Nat Commun. 2021 Mar 12;12(1):1648. (PMID: 33712605) Cell. 2015 May 21;161(5):1202-1214. (PMID: 26000488) Nat Cardiovasc Res. 2022 Dec 21;1(12):1215-1229. (PMID: 36938497) Circulation. 2017 Sep 19;136(12):1123-1139. (PMID: 28733351) Nat Commun. 2022 Dec 27;13(1):7960. (PMID: 36575170) Nat Rev Genet. 2005 Nov;6(11):826-35. (PMID: 16304598) BMC Bioinformatics. 2013 Apr 15;14:128. (PMID: 23586463) Nat Protoc. 2017 Jan;12(1):15-31. (PMID: 27906170) Nat Rev Cardiol. 2018 Nov;15(11):685-704. (PMID: 30287945) Circ Res. 2016 Feb 5;118(3):368-70. (PMID: 26846633) J Histochem Cytochem. 2019 Jun;67(6):453-463. (PMID: 30768367) Cells. 2022 Aug 17;11(16):. (PMID: 36010635) Circ Res. 2020 Jun 5;126(12):1685-1702. (PMID: 32212902) Nat Genet. 2004 Sep;36(9):958-60. (PMID: 15314640) Stem Cell Reports. 2023 Oct 10;18(10):1972-1986. (PMID: 37714147) Cell Rep. 2025 Jan 28;44(1):115091. (PMID: 39709602) Genes Dev. 2003 Aug 15;17(16):1937-56. (PMID: 12893779) Circ Res. 2008 Apr 11;102(7):813-22. (PMID: 18292603) Cardiovasc Res. 2022 Nov 10;118(14):2960-2972. (PMID: 35212715) Cell Rep. 2020 Mar 3;30(9):3149-3163.e6. (PMID: 32130914) Development. 2004 May;131(9):2219-31. (PMID: 15073151) Am J Med Genet A. 2013 Mar;161A(3):572-7. (PMID: 23401077) Eur Heart J. 2020 Sep 21;41(36):3462-3474. (PMID: 32657324) J Pharmacol Sci. 2012;119(3):198-203. (PMID: 22786561) Cardiovasc Res. 2005 Sep 1;67(4):583-93. (PMID: 16002056) Nat Commun. 2021 Jan 29;12(1):681. (PMID: 33514719) PLoS Comput Biol. 2021 Sep 17;17(9):e1009305. (PMID: 34534204) Dev Cell. 2016 Nov 21;39(4):491-507. (PMID: 27840109) J Pharm Anal. 2023 Aug;13(8):894-907. (PMID: 37719196) Dev Cell. 2022 Oct 24;57(20):2426-2443.e6. (PMID: 36283392) Genome Biol. 2019 Dec 23;20(1):296. (PMID: 31870423) Circ Res. 2016 Jun 10;118(12):1880-93. (PMID: 27056912) J Asthma Allergy. 2021 Jan 28;14:71-80. (PMID: 33536765) FASEB J. 2020 Jun;34(6):8625-8640. (PMID: 32374060) Genes Dev. 2018 Oct 1;32(19-20):1344-1357. (PMID: 30254108) Mol Cell Biol. 2004 Jun;24(11):4791-800. (PMID: 15143173) Nature. 2019 Jan;565(7740):505-510. (PMID: 30651639) Eur Heart J. 2019 Aug 7;40(30):2507-2520. (PMID: 31162546) Genome Biol. 2019 Mar 22;20(1):63. (PMID: 30902100) Cell. 2016 May 5;165(4):1012-26. (PMID: 27062923) Nat Commun. 2018 Oct 30;9(1):4435. (PMID: 30375404) Int J Mol Sci. 2021 Mar 23;22(6):. (PMID: 33807107) Clin Perinatol. 2016 Sep;43(3):395-407. (PMID: 27524443) Nature. 2024 Mar;627(8005):854-864. (PMID: 38480880) Gigascience. 2020 Dec 26;9(12):. (PMID: 33367645) Circ Res. 2019 Aug 2;125(4):379-397. (PMID: 31284824) Nat Rev Cardiol. 2020 Aug;17(8):457-473. (PMID: 32231331) Dev Cell. 2016 Nov 21;39(4):480-490. (PMID: 27840107) Circulation. 2020 Nov 10;142(19):1831-1847. (PMID: 32972203) Circ Res. 2026 Jan 2;138(1):e327108. (PMID: 41216707) Cell Stem Cell. 2021 Jan 7;28(1):96-111.e7. (PMID: 33142114) Nat Commun. 2019 Apr 26;10(1):1929. (PMID: 31028265) Curr Protoc. 2021 Mar;1(3):e90. (PMID: 33780170) Cell Rep. 2019 Jul 30;28(5):1346-1361.e4. (PMID: 31365875) Genome Biol. 2025 Jul 17;26(1):209. (PMID: 40676652) Cold Spring Harb Perspect Biol. 2013 Mar 01;5(3):a008292. (PMID: 23457256) Cell Rep. 2018 Jan 16;22(3):600-610. (PMID: 29346760) Circulation. 2023 Jan 24;147(4):324-337. (PMID: 36314132) Dev Cell. 2022 Nov 21;57(22):2517-2532.e6. (PMID: 36347256) Nat Cell Biol. 2015 Aug;17(8):994-1003. (PMID: 26214132) Nat Cardiovasc Res. 2022 Mar;1(3):263-280. (PMID: 35959412) Physiol Rev. 2018 Oct 1;98(4):2453-2475. (PMID: 30156497) Fetal Diagn Ther. 2020;47(5):373-386. (PMID: 31533099) J Physiol. 2003 Apr 15;548(Pt 2):493-505. (PMID: 12640016) Nat Genet. 2020 Jan;52(1):40-47. (PMID: 31844321) Proc Natl Acad Sci U S A. 2020 Sep 1;117(35):21469-21479. (PMID: 32817558) Int J Mol Sci. 2020 Nov 06;21(21):. (PMID: 33172208) Cell Mol Life Sci. 2007 Mar;64(6):704-18. (PMID: 17380311) Immunity. 2022 Sep 13;55(9):1549-1563. (PMID: 36103852) Stem Cell Investig. 2017 Jun 13;4:54. (PMID: 28725650) Circulation. 2016 Dec 6;134(23):1848-1862. (PMID: 27789555) Vascul Pharmacol. 2024 Dec;157:107434. (PMID: 39423955) J Cardiovasc Dev Dis. 2022 Apr 19;9(5):. (PMID: 35621833) Front Cardiovasc Med. 2019 Nov 26;6:165. (PMID: 31850371) Circulation. 2020 Aug 4;142(5):466-482. (PMID: 32403949) Nature. 2020 Dec;588(7838):466-472. (PMID: 32971526) Mol Cells. 2022 Mar 31;45(3):122-133. (PMID: 34887365) Cell Stem Cell. 2020 Oct 1;27(4):574-589.e8. (PMID: 32810435) Stem Cell Reports. 2014 Oct 14;3(4):594-605. (PMID: 25358788) Nat Commun. 2021 May 5;12(1):2551. (PMID: 33953173) Nat Cardiovasc Res. 2025 Oct;4(10):1345-1362. (PMID: 41044418) Dev Cell. 2023 May 8;58(9):791-805.e4. (PMID: 37023750) Cardiovasc Res. 2023 May 2;119(3):772-785. (PMID: 35950218) Cell Mol Life Sci. 2021 Aug;78(16):6033-6049. (PMID: 34274976) Elife. 2021 Aug 17;10:. (PMID: 34402430) Stem Cell Reports. 2018 May 8;10(5):1657-1672. (PMID: 29681541) Circ Res. 2007 Feb 16;100(3):354-62. (PMID: 17234970) Cell Rep. 2019 Feb 12;26(7):1934-1950.e5. (PMID: 30759401) FASEB J. 2015 Apr;29(4):1329-43. (PMID: 25491312) Bioinformatics. 2022 Jun 24;38(Suppl 1):i290-i298. (PMID: 35758781) Nucleic Acids Res. 2016 Jul 8;44(W1):W90-7. (PMID: 27141961) Nat Commun. 2020 May 22;11(1):2585. (PMID: 32444791) Nat Biotechnol. 2021 Jul;39(7):813-818. (PMID: 33795888) Front Cell Dev Biol. 2023 Apr 03;11:1163331. (PMID: 37077417) Nat Genet. 2025 Nov;57(11):2756-2771. (PMID: 41162788) Development. 2019 Mar 12;146(12):. (PMID: 30796046) Am J Dis Child. 1977 Mar;131(3):293-4. (PMID: 842515) Nat Commun. 2020 May 1;11(1):2142. (PMID: 32358524) Am J Physiol Heart Circ Physiol. 2007 Sep;293(3):H1883-91. (PMID: 17604329) iScience. 2023 Nov 30;27(1):108599. (PMID: 38170020) F1000Res. 2021 Sep 28;10:979. (PMID: 35814628) Development. 2005 Mar;132(5):1137-46. (PMID: 15689382) Semin Cell Dev Biol. 2021 Nov;119:49-60. (PMID: 33952430) Clin Sci (Lond). 2022 Aug 12;136(15):1157-1178. (PMID: 35946958) Circ Res. 2016 Feb 5;118(3):400-9. (PMID: 26635390) FEBS Lett. 2016 Feb;590(4):559-70. (PMID: 26801864) Elife. 2022 Nov 28;11:. (PMID: 36441651) Bioinformatics. 2005 Aug 15;21(16):3439-40. (PMID: 16082012) Sci Rep. 2024 Oct 26;14(1):25481. (PMID: 39461968) Nat Methods. 2017 Apr;14(4):395-398. (PMID: 28192419) Circ Cardiovasc Genet. 2011 Apr;4(2):123-33. (PMID: 21282332) Blood. 2016 Aug 25;128(8):e20-31. (PMID: 27365425) Circulation. 2024 Apr 30;149(18):1435-1456. (PMID: 38357822) Cell Rep. 2024 Jan 23;43(1):113616. (PMID: 38150367) Nature. 2019 Aug;572(7767):120-124. (PMID: 31341279) Am J Hum Genet. 2006 Apr;78(4):604-14. (PMID: 16532391) |
| Grant Information: | G-17-0019170 Heart and Stroke Foundation of Canada; MIBAVA-Leducq 12CVD03 Fondation Leducq; Nationale Bank van België; 453653 Canada CAPMC CIHR; 27335 Fonds de Recherche du Québec - Sante; Deutsche Forschungsgemeinschaft; BMBF 01ZZ2004 German Federal Ministry of Education and Research |
| Contributed Indexing: | Keywords: Endocardial development; H19; Heart maturation; Perinatal; Single-cell RNA-seq; Valve development |
| Substance Nomenclature: | 0 (RNA, Long Noncoding) 0 (H19 long non-coding RNA) |
| Entry Date(s): | Date Created: 20260911 Date Completed: 20260911 Latest Revision: 20260926 |
| Update Code: | 20260926 |
| PubMed Central ID: | PMC13602375 |
| DOI: | 10.1242/bio.062493 |
| PMID: | 42725465 |
| Βάση Δεδομένων: | MEDLINE |
καταχωρήστε σχόλιο πρώτοι!