Academic Journal
Fatty acid species differentially regulate macrophage polarization and oxidative stress with secondary effects on macrophage-HSC crosstalk.
| Τίτλος: | Fatty acid species differentially regulate macrophage polarization and oxidative stress with secondary effects on macrophage-HSC crosstalk. |
|---|---|
| Συγγραφείς: | Nakanishi K; Department of Hepato-Biliary-Pancreatic Surgery, Osaka Metropolitan University, Osaka, Japan., Shinkawa H; Department of Hepato-Biliary-Pancreatic Surgery, Osaka Metropolitan University, Osaka, Japan., Takemura S; Department of Frontier Life-Science, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan., Nakagawa K; Department of Frontier Life-Science, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan., Minamiyama Y; Department of Hepato-Biliary-Pancreatic Surgery, Osaka Metropolitan University, Osaka, Japan.; Food Hygiene and Environmental Health Division of Applied Life Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan., Ishizawa T; Department of Hepato-Biliary-Pancreatic Surgery, Osaka Metropolitan University, Osaka, Japan. |
| Πηγή: | Physiological reports [Physiol Rep] 2026 Sep; Vol. 14 (17), pp. e71082. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society Country of Publication: United States NLM ID: 101607800 Publication Model: Print Cited Medium: Internet ISSN: 2051-817X (Electronic) Linking ISSN: 2051817X NLM ISO Abbreviation: Physiol Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [Malden MA] : published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society, 2013- |
| Ιατρικοί όροι (MeSH): | Macrophages*/metabolism , Macrophages*/drug effects , Oxidative Stress*/drug effects , Hepatic Stellate Cells*/metabolism , Hepatic Stellate Cells*/drug effects , Fatty Acids*/metabolism , Fatty Acids*/pharmacology , Palmitic Acid*/pharmacology , Oleic Acid*/pharmacology, Reactive Oxygen Species/metabolism ; PPAR gamma/metabolism ; Fatty Acids, Monounsaturated/pharmacology ; Animals ; Mice ; Macrophage Activation ; Mice, Inbred C57BL ; Cells, Cultured ; Cell Communication |
| Περίληψη: | Metabolic dysfunction-associated steatohepatitis (MASH) is characterized by lipid accumulation, inflammation, and fibrosis. Macrophage-hepatic stellate cell (HSC) communication plays a key role in fibrogenesis; however, how fatty acid species influence macrophage polarization remains unclear. Bone marrow-derived macrophages (BMDMs) were polarized toward M1 or M2 phenotypes in the presence of palmitic acid (PA), oleic acid (OA), or palmitoleic acid (PO). Polarization markers, reactive oxygen species (ROS), and peroxisome proliferator-activated receptor gamma (PPARγ) expression were analyzed by RT-qPCR, Western blotting, and chemiluminescence. Primary HSCs were exposed to fatty acids or conditioned media from treated macrophages. PA enhanced selected inflammatory macrophage responses and increased ROS production under M1-polarizing conditions. OA enhanced ROS production in M1 macrophages and suppressed several M2-associated markers, whereas PO reduced inflammatory responses and ROS under M1-polarizing conditions. Direct fatty acid exposure did not significantly alter HSC activation markers. Conditioned media from polarized macrophages, particularly M1 macrophages, reduced α-SMA and cytoglobin protein expression in HSCs, while fatty acid-specific effects were modest and marker-dependent. Fatty acid species differentially modulate macrophage polarization and redox activity, partly in association with PPARγ signaling. These macrophage changes may secondarily affect macrophage-HSC communication and stellate cell redox-related responses, providing insight into lipid regulation of immune-stromal interactions in the liver. (© 2026 The Author(s). Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.) |
| References: | Trends Endocrinol Metab. 2018 Mar;29(3):178-190. (PMID: 29290500) Life Sci. 2018 Jun 15;203:291-304. (PMID: 29709653) J Clin Invest. 2006 Nov;116(11):3015-25. (PMID: 17053832) J Gastroenterol Hepatol. 2015 Feb;30(2):405-12. (PMID: 25089018) J Lipid Res. 2013 Mar;54(3):725-733. (PMID: 23275648) J Immunol Res. 2019 Nov 11;2019:1278301. (PMID: 31815149) J Hepatol. 2020 Jul;73(1):202-209. (PMID: 32278004) Clin Exp Pharmacol Physiol. 2017 May;44(5):566-575. (PMID: 28135761) Diabetes. 2012 Aug;61(8):2074-83. (PMID: 22586586) PLoS One. 2013 Sep 30;8(9):e75147. (PMID: 24098682) PLoS One. 2015 Jun 01;10(6):e0128218. (PMID: 26030149) J Biol Chem. 2015 Jul 3;290(27):16979-88. (PMID: 25987561) Evid Based Complement Alternat Med. 2021 Jun 22;2021:6671129. (PMID: 34239589) Nature. 2006 Dec 14;444(7121):860-7. (PMID: 17167474) J Leukoc Biol. 2015 Jan;97(1):61-9. (PMID: 25359998) ISRN Endocrinol. 2012;2012:237613. (PMID: 23251812) Front Physiol. 2017 Nov 08;8:902. (PMID: 29167646) J Hepatol. 2001 Aug;35(2):297-306. (PMID: 11580156) Physiol Rev. 2008 Jan;88(1):125-72. (PMID: 18195085) Hepatology. 2010 Nov;52(5):1836-46. (PMID: 21038418) Cells. 2026 Feb 06;15(3):. (PMID: 41677671) Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Oct;1865(10):158776. (PMID: 32738301) Prostaglandins Leukot Essent Fatty Acids. 2021 Jun;169:102270. (PMID: 33930845) J Hepatol. 2020 Oct;73(4):882-895. (PMID: 32330605) Mediators Inflamm. 2014;2014:582197. (PMID: 25147439) Med Sci Monit. 2018 Apr 10;24:2134-2141. (PMID: 29633755) Sci Rep. 2018 Dec 14;8(1):17860. (PMID: 30552362) Am J Physiol Gastrointest Liver Physiol. 2015 Aug 15;309(4):G270-8. (PMID: 26113297) Free Radic Res. 2007 Apr;41(4):489-97. (PMID: 17454131) Scand J Immunol. 2017 Mar;85(3):166-174. (PMID: 27491503) Liver Int. 2005 Apr;25(2):420-8. (PMID: 15780068) J Am Soc Nephrol. 2011 Feb;22(2):317-26. (PMID: 21289217) Am J Physiol Gastrointest Liver Physiol. 2018 Feb 1;314(2):G150-G163. (PMID: 28971836) Arterioscler Thromb Vasc Biol. 2011 Jun;31(6):1397-402. (PMID: 21415385) Food Funct. 2021 Sep 7;12(17):7909-7922. (PMID: 34250536) Chemotherapy. 2013;59(3):214-24. (PMID: 24356281) Biochem Biophys Res Commun. 2021 Dec 10;582:77-85. (PMID: 34695754) |
| Grant Information: | 21K08690 Japan Society for the Promotion of Science (JSPS KAKENHI) |
| Contributed Indexing: | Keywords: PPARγ; hepatic stellate cells; lipid species; macrophage polarization; metabolic dysfunction–associated steatohepatitis |
| Substance Nomenclature: | 0 (Reactive Oxygen Species) 0 (PPAR gamma) 0 (Fatty Acids) 209B6YPZ4I (palmitoleic acid) 2V16EO95H1 (Palmitic Acid) 2UMI9U37CP (Oleic Acid) 0 (Fatty Acids, Monounsaturated) |
| Entry Date(s): | Date Created: 20260911 Date Completed: 20260911 Latest Revision: 20260914 |
| Update Code: | 20260914 |
| PubMed Central ID: | PMC13562811 |
| DOI: | 10.14814/phy2.71082 |
| PMID: | 42723224 |
| Βάση Δεδομένων: | MEDLINE |
καταχωρήστε σχόλιο πρώτοι!