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Acute Toxicity Assessment of Cell-Free Supernatant Derived from Lysinibacillus sp. from Indonesian Peat Soil as a Potential Broad-Spectrum Antibiotic.

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Τίτλος: Acute Toxicity Assessment of Cell-Free Supernatant Derived from Lysinibacillus sp. from Indonesian Peat Soil as a Potential Broad-Spectrum Antibiotic.
Συγγραφείς: Mukti BH, Mahdiyah D, Redjeki DSS, Kandek IA, Putri RS
Πηγή: Pakistan journal of biological sciences : PJBS [Pak J Biol Sci] 2026 Oct; Vol. 29 (6), pp. 300-307.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Asian Network for Scientific Information Country of Publication: Pakistan NLM ID: 101247723 Publication Model: Print Cited Medium: Internet ISSN: 1812-5735 (Electronic) Linking ISSN: 10288880 NLM ISO Abbreviation: Pak J Biol Sci Subsets: MEDLINE
Imprint Name(s): Original Publication: Faisalabad : Asian Network for Scientific Information
Ιατρικοί όροι (MeSH): Anti-Bacterial Agents*/therapeutic use , Anti-Bacterial Agents*/pharmacology , Anti-Bacterial Agents*/adverse effects , Bacillaceae*/metabolism, Toxicity Tests, Acute/methods ; Animals ; Indonesia ; Mice ; Female ; Soil Microbiology ; Soil
Περίληψη: <b>Background and Objective:</b> Antimicrobial resistance (AMR) drives an urgent need for new antibiotics. A <i>Lysinibacillus</i> sp. isolate from Indonesian peat soil has shown promising broad-spectrum antibacterial activity <i>in vitro</i>. This study aims to evaluate the acute toxicity profile of its cell-free supernatant (CFS) as an initial <i>in vivo</i> safety assessment. <b>Materials and Methods:</b> A single oral dose acute toxicity test was conducted on female mice (n = 3 per group). Treatment groups received CFS at volumes of 0.2 mL, 0.5 mL and 1.0 mL. Control groups received 0.5% Na carboxy methyl cellulose (CMC) and nutrient broth (NB). All animals were observed for 14 days for mortality, behavior and body weight changes, followed by necropsy on day 15. Statistical analysis was conducted using SPSS version 26, applying GLM repeated measures with Shapiro-Wilk normality, Mauchly's sphericity and Bonferroni <i>post-hoc</i> tests at a significance level of p<0.05, with results expressed as Mean±SD. <b>Results:</b> There was no mortality or significant behavioral changes. However, macroscopic necropsy revealed dose-dependent toxicity. At a dose of 0.2 mL, heart swelling and tracheal hemorrhage were found. A dose of 0.5 mL caused lung hemorrhage and a pale trachea. At the highest dose of 1.0 mL, all animals showed kidney swelling, lung hemorrhage and pale digestive tract. The Na CMC control group was normal, while the NB control showed mild abnormalities. <b>Conclusion:</b> The CFS of <i>Lysinibacillus</i> sp. did not show LD<sub>50</sub> toxicity or behavioral changes in mice. Some high doses showed organ-specific toxicity.
Contributed Indexing: Keywords: Acute toxicity; Lysinibacillus; antibiotic; cell-free supernatant; mice; peat soil
Substance Nomenclature: 0 (Anti-Bacterial Agents)
0 (Soil)
Entry Date(s): Date Created: 20260911 Date Completed: 20260911 Latest Revision: 20260911
Update Code: 20260911
DOI: 10.3923/pjbs.2026.300.307
PMID: 42723162
Βάση Δεδομένων: MEDLINE
FullText Text:
  Availability: 0
Header DbId: cmedm
DbLabel: MEDLINE
An: 42723162
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
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  Data: Acute Toxicity Assessment of Cell-Free Supernatant Derived from Lysinibacillus sp. from Indonesian Peat Soil as a Potential Broad-Spectrum Antibiotic.
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22101247723%22&quot;&gt;Pakistan journal of biological sciences : PJBS&lt;/searchLink&gt; [Pak J Biol Sci] 2026 Oct; Vol. 29 (6), pp. 300-307.
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  Data: &lt;i&gt;Original Publication&lt;/i&gt;: Faisalabad : Asian Network for Scientific Information
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– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: &amp;lt;b&amp;gt;Background and Objective:&amp;lt;/b&amp;gt; Antimicrobial resistance (AMR) drives an urgent need for new antibiotics. A &amp;lt;i&amp;gt;Lysinibacillus&amp;lt;/i&amp;gt; sp. isolate from Indonesian peat soil has shown promising broad-spectrum antibacterial activity &amp;lt;i&amp;gt;in vitro&amp;lt;/i&amp;gt;. This study aims to evaluate the acute toxicity profile of its cell-free supernatant (CFS) as an initial &amp;lt;i&amp;gt;in vivo&amp;lt;/i&amp;gt; safety assessment. &amp;lt;b&amp;gt;Materials and Methods:&amp;lt;/b&amp;gt; A single oral dose acute toxicity test was conducted on female mice (n = 3 per group). Treatment groups received CFS at volumes of 0.2 mL, 0.5 mL and 1.0 mL. Control groups received 0.5% Na carboxy methyl cellulose (CMC) and nutrient broth (NB). All animals were observed for 14 days for mortality, behavior and body weight changes, followed by necropsy on day 15. Statistical analysis was conducted using SPSS version 26, applying GLM repeated measures with Shapiro-Wilk normality, Mauchly&#39;s sphericity and Bonferroni &amp;lt;i&amp;gt;post-hoc&amp;lt;/i&amp;gt; tests at a significance level of p&amp;lt;0.05, with results expressed as Mean&#177;SD. &amp;lt;b&amp;gt;Results:&amp;lt;/b&amp;gt; There was no mortality or significant behavioral changes. However, macroscopic necropsy revealed dose-dependent toxicity. At a dose of 0.2 mL, heart swelling and tracheal hemorrhage were found. A dose of 0.5 mL caused lung hemorrhage and a pale trachea. At the highest dose of 1.0 mL, all animals showed kidney swelling, lung hemorrhage and pale digestive tract. The Na CMC control group was normal, while the NB control showed mild abnormalities. &amp;lt;b&amp;gt;Conclusion:&amp;lt;/b&amp;gt; The CFS of &amp;lt;i&amp;gt;Lysinibacillus&amp;lt;/i&amp;gt; sp. did not show LD&amp;lt;sub&amp;gt;50&amp;lt;/sub&amp;gt; toxicity or behavioral changes in mice. Some high doses showed organ-specific toxicity.
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  Data: &lt;i&gt;Keywords: &lt;/i&gt;Acute toxicity; Lysinibacillus; antibiotic; cell-free supernatant; mice; peat soil
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  Data: 0 (Anti-Bacterial Agents)&lt;br /&gt;0 (Soil)
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  Data: &lt;i&gt;Date Created: &lt;/i&gt;20260911 &lt;i&gt;Date Completed: &lt;/i&gt;20260911 &lt;i&gt;Latest Revision: &lt;/i&gt;20260911
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PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42723162
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.3923/pjbs.2026.300.307
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 300
    Subjects:
      – SubjectFull: Toxicity Tests, Acute methods
        Type: general
      – SubjectFull: Animals
        Type: general
      – SubjectFull: Indonesia
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      – SubjectFull: Mice
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      – SubjectFull: Female
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      – SubjectFull: Soil Microbiology
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      – SubjectFull: Anti-Bacterial Agents therapeutic use
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      – SubjectFull: Anti-Bacterial Agents adverse effects
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      – SubjectFull: Bacillaceae metabolism
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      – TitleFull: Acute Toxicity Assessment of Cell-Free Supernatant Derived from Lysinibacillus sp. from Indonesian Peat Soil as a Potential Broad-Spectrum Antibiotic.
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            NameFull: Mukti BH
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              M: 10
              Text: 2026 Oct
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