Academic Journal
Προβολή σε EDS
Συνδεδεμένο Πλήρες Κείμενο

Molecular divergence and genomic composition of B chromosomes in the fish Cyphocharax modestus (Characiformes, Curimatidae).

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Molecular divergence and genomic composition of B chromosomes in the fish Cyphocharax modestus (Characiformes, Curimatidae).
Συγγραφείς: Dos Santos N; Faculdade de Ciências, Universidade Estadual Paulista, Bauru, SP, 17033-360, Brazil., Sassi FMC; Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, 13565-905, Brazil.; Aquaculture Center of Unesp, São Paulo State University (Unesp), Jaboticabal - SP, 14884-900, Brazil., Dos Santos RZ; Faculdade de Ciências, Universidade Estadual Paulista, Bauru, SP, 17033-360, Brazil., Goes CAG; Faculdade de Ciências, Universidade Estadual Paulista, Bauru, SP, 17033-360, Brazil., de Moraes RLR; Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, 13565-905, Brazil., Porto-Foresti F; Faculdade de Ciências, Universidade Estadual Paulista, Bauru, SP, 17033-360, Brazil., Liehr T; Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany. thomas.liehr@med.uni-jena.de., Cioffi MB; Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, 13565-905, Brazil., Utsunomia R; Faculdade de Ciências, Universidade Estadual Paulista, Bauru, SP, 17033-360, Brazil.
Πηγή: Scientific reports [Sci Rep] 2026 Sep 09; Vol. 16 (1). Date of Electronic Publication: 2026 Sep 09.
Τύπος έκδοσης: Journal Article; Research Support, Non-U.S. Gov't
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Imprint Name(s): Original Publication: London : Nature Publishing Group, copyright 2011-
Ιατρικοί όροι (MeSH): Characiformes*/genetics , Chromosomes*/genetics , Genetic Variation* , Genome*, DNA, Satellite/genetics ; Animals ; Karyotype ; In Situ Hybridization, Fluorescence ; Comparative Genomic Hybridization ; Evolution, Molecular ; Karyotyping ; Genomics ; Phylogeny
Περίληψη: B chromosomes are supernumerary elements that evolve from standard A chromosomes and are primarily composed of repetitive DNAs, yet their origin, diversification, and molecular composition remain poorly understood in most vertebrates. We investigated two allopatric populations of Cyphocharax modestus (Curimatidae) combining classical cytogenetics, comparative genomic hybridization (CGH), and comparative satellitomics to characterize the repetitive DNA landscape of its B chromosomes. While both populations exhibited a conserved karyotype of 2n=54 biarmed chromosomes, five individuals from the Batalha River (BR) carried supernumerary chromosomes, comprising two distinct variants: a C-positive B1 and an C-negative B2. Comparative satellitome analysis between 3B-carrying and B-lacking individuals identified 116 satellite DNAs (CmoSatDNAs), with the 3B library showing higher abundances of specific sequences. Fluorescence in situ hybridization (FISH) revealed that both B variants share two centromeric satellites (CmoSat01-192 and CmoSat02-108) with the A complement, while CmoSat58-47 was exclusively to B2. Minimum spanning tree analysis of CmoSat58-47 revealed B-exclusive haplotypes alongside haplotypes shared with B-lacking individuals, suggesting a recent origin for these chromosomes. CGH experiments further confirm the sequence sharing between the A and B chromosomes, supporting an intraspecific origin, and revealing substantial genomic differentiation among B variants.
(© 2026. The Author(s).)
Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Ethics approval: Sample was approved by the Brazilian Environmental Agency ICMBIO/SISBIO (License 3245). Allexperiments followed the guidelines and were approved by the Ethics Committee on the Use of Animals at SãoPaulo State University (IBB/UNESP), under protocol 1204-CEUA/2019. The authors complied with ARRIVEguidelines
References: Prog Mol Subcell Biol. 2021;60:85-102. (PMID: 34386873)
BMC Biol. 2013 Aug 06;11:90. (PMID: 23915065)
Cells. 2021 May 11;10(5):. (PMID: 34064768)
Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404)
Genet Mol Biol. 2022 Jun 27;45(2):e20210125. (PMID: 35766400)
Nucleic Acids Res. 2017 Jul 07;45(12):e111. (PMID: 28402514)
J Exp Bot. 2021 Feb 27;72(5):1606-1616. (PMID: 33216934)
Chromosoma. 2017 Aug;126(4):487-500. (PMID: 27522227)
Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286)
PLoS One. 2011 Mar 09;6(3):e17288. (PMID: 21408061)
BMC Biol. 2022 Feb 7;20(1):36. (PMID: 35130900)
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7. (PMID: 15034147)
Trends Plant Sci. 2003 Sep;8(9):417-23. (PMID: 13678908)
Mol Biol Evol. 2017 Dec 1;34(12):3299-3302. (PMID: 29029172)
Chromosoma. 2017 Feb;126(1):73-81. (PMID: 27558128)
Cytogenet Genome Res. 2004;106(2-4):230-4. (PMID: 15292596)
Philos Trans R Soc Lond B Biol Sci. 2000 Feb 29;355(1394):163-78. (PMID: 10724453)
Sci Rep. 2016 Jul 07;6:28333. (PMID: 27385065)
Proc Natl Acad Sci U S A. 2012 Aug 14;109(33):13343-6. (PMID: 22847450)
Cytogenet Genome Res. 2021;161(5):249-256. (PMID: 34433167)
Comp Cytogenet. 2011 Nov 09;5(4):301-13. (PMID: 24260637)
PLoS One. 2014 Apr 15;9(4):e94896. (PMID: 24736529)
Cell Mol Life Sci. 2014 Feb;71(3):467-78. (PMID: 23912901)
Cytogenet Genome Res. 2011;135(2):135-42. (PMID: 21952475)
Plant Cell Rep. 2009 Dec;28(12):1857-67. (PMID: 19847437)
PLoS One. 2016 Mar 02;11(3):e0150573. (PMID: 26934481)
Genes (Basel). 2018 Jul 31;9(8):. (PMID: 30065230)
Genome Dyn. 2012;7:126-52. (PMID: 22759817)
Cytogenet Genome Res. 2014;144(3):220-6. (PMID: 25612643)
Heredity (Edinb). 2005 Aug;95(2):118-28. (PMID: 15931241)
Genetics. 2018 Dec;210(4):1197-1212. (PMID: 30249684)
Genetika. 2010 Sep;46(9):1234-6. (PMID: 21061624)
Genes (Basel). 2019 Feb 18;10(2):. (PMID: 30781667)
Chromosome Res. 2022 Sep;30(2-3):229-239. (PMID: 35412169)
Cytogenet Genome Res. 2015;146(1):51-7. (PMID: 25999244)
Heredity (Edinb). 2000 Jul;85 ( Pt 1):1-9. (PMID: 10971685)
BMC Biol. 2021 Mar 19;19(1):52. (PMID: 33740955)
Braz J Biol. 2007 Dec;67(4 Suppl):905-8. (PMID: 18278358)
Cells. 2021 Jun 17;10(6):. (PMID: 34204462)
Cytogenet Cell Genet. 1981;31(3):137-44. (PMID: 6173166)
Exp Cell Res. 2020 Apr 15;389(2):111895. (PMID: 32035948)
Genes (Basel). 2019 Mar 16;10(3):. (PMID: 30884847)
Genes (Basel). 2018 Oct 26;9(11):. (PMID: 30373193)
Nat Rev Genet. 2015 Apr;16(4):213-23. (PMID: 25732611)
Front Plant Sci. 2017 Feb 15;8:210. (PMID: 28261259)
Genes (Basel). 2017 Oct 25;8(11):. (PMID: 29068386)
An Acad Bras Cienc. 2013;85(4):1371-7. (PMID: 24141415)
Comp Cytogenet. 2016 Jan 22;10(1):77-95. (PMID: 27186339)
Exp Cell Res. 1972 Nov;75(1):304-6. (PMID: 4117921)
Cytogenet Genome Res. 2009;125(2):132-41. (PMID: 19729917)
Nat Commun. 2022 Jun 20;13(1):3525. (PMID: 35725842)
Mol Biol Evol. 2014 Aug;31(8):2061-72. (PMID: 24770715)
Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):. (PMID: 34088847)
Cytogenet Genome Res. 2020;160(11-12):711-718. (PMID: 33752199)
Bioinformatics. 2017 Jan 1;33(1):128-129. (PMID: 27605102)
Genome Biol. 2025 Mar 06;26(1):47. (PMID: 40050975)
Genome Biol Evol. 2020 Mar 1;12(3):88-102. (PMID: 32211863)
Mol Genet Genomics. 2020 Jan;295(1):195-207. (PMID: 31624915)
PLoS One. 2014 Mar 14;9(3):e91820. (PMID: 24632855)
Contributed Indexing: Keywords: Comparative genomic hybridization; Cytogenomic; Repetitive DNAs; Satellitome; Supernumerary chromosomes; satDNAs
Substance Nomenclature: 0 (DNA, Satellite)
Entry Date(s): Date Created: 20260910 Date Completed: 20260910 Latest Revision: 20260917
Update Code: 20260917
PubMed Central ID: PMC13558729
DOI: 10.1038/s41598-026-66448-7
PMID: 42717220
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:2045-2322
DOI:10.1038/s41598-026-66448-7