Academic Journal
Global diffusion of IncC plasmid harboring blaNDM-1 in the high-risk Escherichia coli ST131 clone.
| Τίτλος: | Global diffusion of IncC plasmid harboring blaNDM-1 in the high-risk Escherichia coli ST131 clone. |
|---|---|
| Συγγραφείς: | Bouhalouene K; Biology Department, Faculty of Sciences of Bizerte, University of Carthage, Jarzouna 7021, Tunisia.; Laboratory Department, National Bone Marrow Transplant Center, 1006 Tunis, Tunisia.; Biology Department, Faculty of Medicine of Tunis, University of Tunis El Manar, LR18ES39, 1007 Tunis, Tunisia., Raddaoui A; Laboratory Department, National Bone Marrow Transplant Center, 1006 Tunis, Tunisia.; Biology Department, Faculty of Medicine of Tunis, University of Tunis El Manar, LR18ES39, 1007 Tunis, Tunisia., Chebbi Y; Laboratory Department, National Bone Marrow Transplant Center, 1006 Tunis, Tunisia.; Biology Department, Faculty of Medicine of Tunis, University of Tunis El Manar, LR18ES39, 1007 Tunis, Tunisia., Achour W; Laboratory Department, National Bone Marrow Transplant Center, 1006 Tunis, Tunisia.; Biology Department, Faculty of Medicine of Tunis, University of Tunis El Manar, LR18ES39, 1007 Tunis, Tunisia. |
| Πηγή: | Journal of applied microbiology [J Appl Microbiol] 2026 Oct 01; Vol. 137 (10). |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2022- : Oxford : Oxford University Press Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997- |
| Ιατρικοί όροι (MeSH): | Escherichia coli*/genetics , Escherichia coli*/drug effects , Escherichia coli*/enzymology , Escherichia coli*/isolation & purification , beta-Lactamases*/genetics , Plasmids*/genetics , Drug Resistance, Multiple, Bacterial*/genetics , Escherichia coli Infections*/microbiology, Anti-Bacterial Agents/pharmacology ; Quinolones/pharmacology ; Fluoroquinolones/pharmacology ; Humans ; Microbial Sensitivity Tests ; Multilocus Sequence Typing ; beta-Lactam Resistance |
| Περίληψη: | Aims: The global expansion of quinolone-resistant Escherichia coli (QR-EC) is increasingly associated with β-lactam resistance and mobile genetic elements that facilitate resistance dissemination. This study investigated the molecular mechanisms underlying fluoroquinolone and β-lactam resistance in clinical QR-EC isolates and explored the plasmid type associated. Methods and Results: A total of 123 non-duplicate QR-EC clinical isolates responsible mainly for gastrointestinal colonization were collected between 2019 and 2021. Plasmid-mediated quinolone resistance (PMQR), extended-spectrum β-lactamase (ESBL), and carbapenemase genes were screened by PCR. Mutations in the quinolone resistance-determining regions (QRDR) of gyrA and parC were analyzed using sequencing and mismatch amplification mutation assay PCR (MAMA-PCR). Selected isolates underwent multilocus sequence typing (MLST). Whole-genome sequencing (WGS) of a representative extensively drug-resistant strain carrying multiple quinolone resistance determinants, ESBL genes, and carbapenemase genes, was performed. PMQR genes were prevalent among QR-EC, dominated by aac(6')-Ib-cr (60.9% of isolates). ESBL genes were identified in 93.5% of isolates, predominantly blaCTX-M (95.7%). Among ertapenem-resistant isolates (QCR-EC) (n = 18), blaNDM-1 and blaOXA-48 were detected in 13 and 11 isolates, respectively. QRDR mutations were highly frequent, particularly gyrA83 (98.4%) and parC80 (30.9%). Major QCR-EC genotypes belonged to sequence types ST167 (n = 2), ST1196, ST469, and ST410. High-risk E. coli ST131 clone harboring IncC plasmid encoding blaNDM-1 was described for the first time in Africa, following its emergence, in two continents, Asia and America. Despite the very rare description of these strains worldwide, their description in three continents sign their global diffusion. Conclusions: This finding highlights the ongoing spread of carbapenem resistance and underscores the urgent need for strengthened genomic surveillance. (© The Author(s) 2026. Published by Oxford University Press on behalf of Applied Microbiology International. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com.) |
| Contributed Indexing: | Keywords: Escherichia coli; IncC plasmid; NDM-1; ST131; carbapenemase; quinolone-resistance |
| Substance Nomenclature: | EC 3.5.2.6 (beta-Lactamases) 0 (Anti-Bacterial Agents) EC 3.5.2.6 (beta-lactamase NDM-1) 0 (Quinolones) 0 (Fluoroquinolones) |
| Entry Date(s): | Date Created: 20260909 Date Completed: 20261008 Latest Revision: 20261008 |
| Update Code: | 20261009 |
| DOI: | 10.1093/jambio/lxag229 |
| PMID: | 42714829 |
| Βάση Δεδομένων: | MEDLINE |
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