Academic Journal
Protein profiling and GC-MS product analysis provide insights into lignite solubilization and bioconversion by Lysinibacillus sphaericus strain SH19.
| Τίτλος: | Protein profiling and GC-MS product analysis provide insights into lignite solubilization and bioconversion by Lysinibacillus sphaericus strain SH19. |
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| Συγγραφείς: | Cheng J; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China. cjg143@126.com., Zhang Z; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Chen P; College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010021, People's Republic of China., Zhao Y; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., He Z; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Li Q; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Yang Z; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Zhang S; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Liu Y; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Duan J; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Wang X; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China. |
| Πηγή: | Archives of microbiology [Arch Microbiol] 2026 Sep 08; Vol. 208 (12). Date of Electronic Publication: 2026 Sep 08. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0410427 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-072X (Electronic) Linking ISSN: 03028933 NLM ISO Abbreviation: Arch Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Berlin, New York, Springer-Verlag. |
| Ιατρικοί όροι (MeSH): | Bacillaceae*/metabolism , Bacillaceae*/genetics , Bacillaceae*/chemistry , Bacillaceae*/isolation & purification , Bacterial Proteins*/metabolism , Bacterial Proteins*/chemistry , Coal*/microbiology , Coal*/analysis, Gas Chromatography-Mass Spectrometry ; Solubility ; Tandem Mass Spectrometry ; Biotransformation ; Electrophoresis, Gel, Two-Dimensional ; Proteomics |
| Περίληψη: | Lignite biosolubilization offers a mild route for valorizing low-rank coal, although the microbial processes that accompany solubilization remain incompletely defined. Here, an endogenous isolate designated Lysinibacillus sphaericus strain SH19 was evaluated using nitric-acid-pretreated Shengli lignite. Under the selected working conditions (4 M nitric-acid pretreatment, initial pH 8, 40°C, and 16 days), the apparent solubilization rate reached 66.81%. Changes in A450, residual solid mass, culture pH, and extracellular protein concentration showed that chemical pretreatment and bacterial culture were both associated with the release of soluble lignite-derived material. SDS-PAGE and two-dimensional electrophoresis revealed treatment-associated differences in extracellular and intracellular protein patterns. LC-MS/MS analysis of excised protein spots yielded 85 candidate protein assignments; the revised supplementary table reports PEAKS scores, sequence coverage, peak area, and unique-peptide counts and highlights the limited support for several entries. GC-MS analysis produced 33 tentative library assignments in the solubilized fraction, but siloxane- and silyl-related signals were treated as possible analytical background, and no pathway was inferred from these assignments alone. Together, the data identify strain SH19 as a promising lignite-biosolubilizing isolate and provide candidate proteins and product signals for future validation. The proposed process model remains exploratory because direct enzyme assays, inhibitor experiments, carbon-balance measurements, transcriptomic or genetic validation, complete GC-MS blank subtraction, and authentic-standard confirmation were not available. (© 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
| Competing Interests: | Declarations. Conflict of interest: The authors declare no competing interests. |
| References: | Akimbekov N, Digel I, Abdieva G et al (2021) Lignite biosolubilization and bioconversion by Bacillus sp.: the collation of analytical data. Biofuels 12:247–258. https://doi.org/10.1080/17597269.2020.1753936. (PMID: 10.1080/17597269.2020.1753936) Akimbekov N, Digel I, Qiao X et al (2020) Lignite biosolubilization by Bacillus sp. RKB 2 and characterization of its products. Geomicrobiol J 37:255–261. https://doi.org/10.1080/01490451.2019.1695022. (PMID: 10.1080/01490451.2019.1695022) Altschul SF, Gish W, Miller W et al (1990) Basic local alignment search tool. J Mol Biol 215:403–410. https://doi.org/10.1016/S0022-2836(05)80360-2. (PMID: 10.1016/S0022-2836(05)80360-22231712) Başaran Y, Denizli A, Sakintuna B et al (2003) Bio-liquefaction/solubilization of low-rank Turkish lignites and characterization of the products. Energy Fuels 17:1068–1074. https://doi.org/10.1021/ef020210s. (PMID: 10.1021/ef020210s) Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254. https://doi.org/10.1016/0003-2697(76)90527-3. (PMID: 10.1016/0003-2697(76)90527-3942051) Cohen MS, Gabriele PD (1982) Degradation of coal by the fungi Polyporus versicolor and Poria monticola. Appl Environ Microbiol 44:23–27. https://doi.org/10.1128/AEM.44.1.23-27.1982. (PMID: 10.1128/AEM.44.1.23-27.198216346060241962) Fakoussa RM, Hofrichter M (1999) Biotechnology and microbiology of coal degradation. Appl Microbiol Biotechnol 52:25–40. https://doi.org/10.1007/s002530051483. (PMID: 10.1007/s00253005148310461367) Feng X, Sun JH, Xie YH (2021) Degradation of Shanxi lignite by Trichoderma citrinoviride. Fuel 291:120204. https://doi.org/10.1016/j.fuel.2021.120204. (PMID: 10.1016/j.fuel.2021.120204) Gao TG, Jiang F, Yang JS et al (2012) Biodegradation of Leonardite by an alkali-producing bacterial community and characterization of the degraded products. Appl Microbiol Biotechnol 93:2581–2590. https://doi.org/10.1007/s00253-011-3669-5. (PMID: 10.1007/s00253-011-3669-522075634) He H, Cheng J, Song DX et al (2022) Impacts of surfactant on bituminous coal biosolubilization by white-rot fungi Hypocrea lixii HN-1. Geomicrobiol J 39:750–756. https://doi.org/10.1080/01490451.2022.2074574. (PMID: 10.1080/01490451.2022.2074574) Hofrichter M, Ziegenhagen D, Sorge S et al (1999) Degradation of lignite (low-rank coal) by ligninolytic basidiomycetes and their manganese peroxidase system. Appl Microbiol Biotechnol 52:78–84. https://doi.org/10.1007/s002530051490. (PMID: 10.1007/s00253005149010461373) Iriani P, Allison GG, Bugg TDH (2025) Degradation of brown coal and 1-methylnaphthalene by a Micrococcus luteus isolate: investigation of a novel aromatic degradation gene cluster containing paa genes. J Appl Microbiol 136:lxaf153. https://doi.org/10.1093/jambio/lxaf153. (PMID: 10.1093/jambio/lxaf15340578825) Jiang F, Li ZH, Lv ZW et al (2013) The biosolubilization of lignite by Bacillus sp. Y7 and characterization of the soluble products. Fuel 103:639–645. https://doi.org/10.1016/j.fuel.2012.08.030. (PMID: 10.1016/j.fuel.2012.08.030) Kang HL, Liu XR, Zhang YW et al (2021) Bacteria solubilization of Shenmu lignite: influence of surfactants and characterization of the biosolubilization products. Energ Source Part A 43:1162–1180. https://doi.org/10.1080/15567036.2019.1635664. (PMID: 10.1080/15567036.2019.1635664) Laborda F, Fernández M, Luna N et al (1997) Study of the mechanisms by which microorganisms solubilize and/or liquefy Spanish coals. Fuel Process Technol 52:95–107. https://doi.org/10.1016/S0378-3820(97)00019-2. (PMID: 10.1016/S0378-3820(97)00019-2) Laborda F, Monistrol IF, Luna N et al (1999) Processes of liquefaction/solubilization of Spanish coals by microorganisms. Appl Microbiol Biotechnol 52:49–56. https://doi.org/10.1007/s002530051485. (PMID: 10.1007/s00253005148510461369) Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685. https://doi.org/10.1038/227680a0. (PMID: 10.1038/227680a05432063) Li SY, Li F, Li PP et al (2023) Bio-solubilization of Yunnan lignite by Penicillium ortum MJ51 and characterization of its products. Fuel 331:125923. https://doi.org/10.1016/j.fuel.2022.125923. (PMID: 10.1016/j.fuel.2022.125923) Ma XM, Zhang MX, Min FF (2014) Study of enhanced low-quality coal oxidative desulphurization and deashing by using HNO<sub>3</sub> and microwave pretreatment. Environ Technol 35:36–41. https://doi.org/10.1080/09593330.2013.808248. (PMID: 10.1080/09593330.2013.80824824600838) Machnikowska H, Pawelec K, Podgórska A (2002) Microbial degradation of low rank coals. Fuel Process Technol 77–78:17–23. https://doi.org/10.1016/S0378-3820(02)00064-4. (PMID: 10.1016/S0378-3820(02)00064-4) Mangu JCK, Rai N, Mandal A et al (2022) Lysinibacillus sphaericus mediates stress responses and attenuates arsenic toxicity in Caenorhabditis elegans. Sci Total Environ 835:155377. https://doi.org/10.1016/j.scitotenv.2022.155377. (PMID: 10.1016/j.scitotenv.2022.15537735460794) Niu X, Zhang JB, Suo YL et al (2022) Proteomic analysis of Fusarium sp. NF01 revealed a multi-level regulatory machinery for lignite biodegradation. Energy 250:123763. https://doi.org/10.1016/j.energy.2022.123763. (PMID: 10.1016/j.energy.2022.123763) Niu Y, Suo YL, Niu X (2023) Insights into the response mechanism of Fusarium sp. NF01 during lignite biodegradation using proteomic analysis. Energy 278:127990. https://doi.org/10.1016/j.energy.2023.127990. (PMID: 10.1016/j.energy.2023.127990) Nussipov D, Akimbekov N, Tastambek K et al (2026) Resource recovery from low-rank coal and livestock manure for sustainable agroecosystems: a review. Arch Microbiol 208:204. https://doi.org/10.1007/s00203-026-04758-0. (PMID: 10.1007/s00203-026-04758-04171190312920336) O’Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021. https://doi.org/10.1016/S0021-9258(19)41496-8. (PMID: 10.1016/S0021-9258(19)41496-82363082874754) Quigley DR, Wey JE, Breckenridge CR et al (1988) The influence of pH on biological solubilization of oxidized, low-rank coal. Resour Conserv Recycl 1:163–174. https://doi.org/10.1016/0921-3449(88)90013-4. (PMID: 10.1016/0921-3449(88)90013-4) Romanowska I, Strzelecki B, Bielecki S (2015) Biosolubilization of Polish brown coal by Gordonia alkanivorans S7 and Bacillus mycoides NS1020. Fuel Process Technol 131:430–436. https://doi.org/10.1016/j.fuproc.2014.12.019. (PMID: 10.1016/j.fuproc.2014.12.019) Sekhohola LM, Igbinigie EE, Cowan AK (2013) Biological degradation and solubilisation of coal. Biodegradation 24:305–318. https://doi.org/10.1007/s10532-012-9594-1. (PMID: 10.1007/s10532-012-9594-123001629) Sekhohola-Dlamini LM, Khan S, Wang BB et al (2025) Recent progress on the biological degradation and solubilization of coal. Biodegradation 36:84. https://doi.org/10.1007/s10532-025-10175-9. (PMID: 10.1007/s10532-025-10175-94095251912436530) Shi C, Liu XR, Zhao SS et al (2023a) Additions of esterase and rhamnolipid promote the degradation of Inner Mongolia coal by Pseudomonas japonica. Fuel 340:127640. https://doi.org/10.1016/j.fuel.2023.127640. (PMID: 10.1016/j.fuel.2023.127640) Shi C, Liu XR, Wu H et al (2023b) Enhancements of mixed surfactants on Wucaiwan coal biodegradation by Nocardia mangyaensis. Energy 266:126501. https://doi.org/10.1016/j.energy.2022.126501. (PMID: 10.1016/j.energy.2022.126501) Shi KY, Tao XX, Yin SD et al (2009) Bio-liquefaction of Fushun lignite: characterization of newly isolated lignite liquefying fungus and liquefaction products. Procedia Earth Planet Sci 1:627–633. https://doi.org/10.1016/j.proeps.2009.09.099. (PMID: 10.1016/j.proeps.2009.09.099) Shi KY, Yin SD, Tao XX et al (2013) Quantitative measurement of coal bio-solubilization by ultraviolet-visible spectroscopy. Energ Source Part A 35:1456–1462. https://doi.org/10.1080/15567036.2010.521800. (PMID: 10.1080/15567036.2010.521800) Shi KY, Gui XH, Tao XX et al (2015) Macromolecular structural unit construction of Fushun nitric-acid-oxidized coal. Energy Fuels 29:3566–3572. https://doi.org/10.1021/ef502859r. (PMID: 10.1021/ef502859r) Sobolczyk-Bednarek J, Choińska-Pulit A, Łaba W (2021) Biosolubilization of low-rank coal by the newly isolated strain Streptomyces fulvissimus K59. Fuel 301:121082. https://doi.org/10.1016/j.fuel.2021.121082. (PMID: 10.1016/j.fuel.2021.121082) Tamura K, Stecher G, Kumar S (2021) MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 38:3022–3027. https://doi.org/10.1093/molbev/msab120. (PMID: 10.1093/molbev/msab120338924918233496) Vigil TN, Johnson GC, Jacob SG et al (2024) Microbial mineralization with Lysinibacillus sphaericus for selective lithium nanoparticle extraction. Environ Sci Technol 58:16915–16921. https://doi.org/10.1021/acs.est.4c06540. (PMID: 10.1021/acs.est.4c065403926382611447963) Wingfield PT (2001) Protein precipitation using ammonium sulfate. Curr Protoc Protein Sci. https://doi.org/10.1002/0471140864.psa03fs13. (PMID: 10.1002/0471140864.psa03fs13184291954821428) Yang J, Liu XR, Yang ZW et al (2023) Biodegradation of Dananhu low-rank coal by Planomicrobium huatugouensis: target metabolites possessing degradation abilities and their biodegradation pathways. Energy 276:127642. https://doi.org/10.1016/j.energy.2023.127642. (PMID: 10.1016/j.energy.2023.127642) Yin SD, Tao XX, Shi KY (2009) Bio-solubilization of Chinese lignite II: protein adsorption onto the lignite surface. Min Sci Technol (China) 19:363–368. https://doi.org/10.1016/S1674-5264(09)60068-5. (PMID: 10.1016/S1674-5264(09)60068-5) Yuan HL, Yang JS, Chen WX (2006) Production of alkaline materials, surfactants and enzymes by Penicillium decumbens strain P6 in association with lignite degradation/solubilization. Fuel 85:1378–1382. https://doi.org/10.1016/j.fuel.2005.12.003. (PMID: 10.1016/j.fuel.2005.12.003) Zhao YL, Chai RN, Duan JG et al (2024) Isolation of native microorganisms from Shengli lignite and study on their ability to dissolve lignite. Bioprocess Biosyst Eng 47:1985–1997. https://doi.org/10.1007/s00449-024-03080-7. (PMID: 10.1007/s00449-024-03080-739127829) |
| Grant Information: | 2022QN02007 Natural Science Foundation of Inner Mongolia Autonomous Region; 2023QN02012 Natural Science Foundation of Inner Mongolia Autonomous Region |
| Contributed Indexing: | Keywords: Lysinibacillus sphaericus strain SH19; GC–MS; LC–MS/MS; Lignite bioconversion; Lignite biosolubilization; Protein profiling |
| Substance Nomenclature: | 0 (Bacterial Proteins) 0 (Coal) |
| SCR Organism: | Lysinibacillus sphaericus |
| Entry Date(s): | Date Created: 20260908 Date Completed: 20260908 Latest Revision: 20260908 |
| Update Code: | 20260909 |
| DOI: | 10.1007/s00203-026-05162-4 |
| PMID: | 42709201 |
| Βάση Δεδομένων: | MEDLINE |
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