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Protein profiling and GC-MS product analysis provide insights into lignite solubilization and bioconversion by Lysinibacillus sphaericus strain SH19.

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Τίτλος: Protein profiling and GC-MS product analysis provide insights into lignite solubilization and bioconversion by Lysinibacillus sphaericus strain SH19.
Συγγραφείς: Cheng J; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China. cjg143@126.com., Zhang Z; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Chen P; College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010021, People's Republic of China., Zhao Y; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., He Z; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Li Q; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Yang Z; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Zhang S; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Liu Y; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Duan J; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China., Wang X; School of Chemistry and Chemical Engineering, Inner Mongolia University of Science and Technology, Baotou, 014010, Inner Mongolia, People's Republic of China.
Πηγή: Archives of microbiology [Arch Microbiol] 2026 Sep 08; Vol. 208 (12). Date of Electronic Publication: 2026 Sep 08.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0410427 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-072X (Electronic) Linking ISSN: 03028933 NLM ISO Abbreviation: Arch Microbiol Subsets: MEDLINE
Imprint Name(s): Original Publication: Berlin, New York, Springer-Verlag.
Ιατρικοί όροι (MeSH): Bacillaceae*/metabolism , Bacillaceae*/genetics , Bacillaceae*/chemistry , Bacillaceae*/isolation & purification , Bacterial Proteins*/metabolism , Bacterial Proteins*/chemistry , Coal*/microbiology , Coal*/analysis, Gas Chromatography-Mass Spectrometry ; Solubility ; Tandem Mass Spectrometry ; Biotransformation ; Electrophoresis, Gel, Two-Dimensional ; Proteomics
Περίληψη: Lignite biosolubilization offers a mild route for valorizing low-rank coal, although the microbial processes that accompany solubilization remain incompletely defined. Here, an endogenous isolate designated Lysinibacillus sphaericus strain SH19 was evaluated using nitric-acid-pretreated Shengli lignite. Under the selected working conditions (4 M nitric-acid pretreatment, initial pH 8, 40°C, and 16 days), the apparent solubilization rate reached 66.81%. Changes in A450, residual solid mass, culture pH, and extracellular protein concentration showed that chemical pretreatment and bacterial culture were both associated with the release of soluble lignite-derived material. SDS-PAGE and two-dimensional electrophoresis revealed treatment-associated differences in extracellular and intracellular protein patterns. LC-MS/MS analysis of excised protein spots yielded 85 candidate protein assignments; the revised supplementary table reports PEAKS scores, sequence coverage, peak area, and unique-peptide counts and highlights the limited support for several entries. GC-MS analysis produced 33 tentative library assignments in the solubilized fraction, but siloxane- and silyl-related signals were treated as possible analytical background, and no pathway was inferred from these assignments alone. Together, the data identify strain SH19 as a promising lignite-biosolubilizing isolate and provide candidate proteins and product signals for future validation. The proposed process model remains exploratory because direct enzyme assays, inhibitor experiments, carbon-balance measurements, transcriptomic or genetic validation, complete GC-MS blank subtraction, and authentic-standard confirmation were not available.
(© 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Competing Interests: Declarations. Conflict of interest: The authors declare no competing interests.
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Grant Information: 2022QN02007 Natural Science Foundation of Inner Mongolia Autonomous Region; 2023QN02012 Natural Science Foundation of Inner Mongolia Autonomous Region
Contributed Indexing: Keywords: Lysinibacillus sphaericus strain SH19; GC–MS; LC–MS/MS; Lignite bioconversion; Lignite biosolubilization; Protein profiling
Substance Nomenclature: 0 (Bacterial Proteins)
0 (Coal)
SCR Organism: Lysinibacillus sphaericus
Entry Date(s): Date Created: 20260908 Date Completed: 20260908 Latest Revision: 20260908
Update Code: 20260909
DOI: 10.1007/s00203-026-05162-4
PMID: 42709201
Βάση Δεδομένων: MEDLINE
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  Data: Protein profiling and GC-MS product analysis provide insights into lignite solubilization and bioconversion by Lysinibacillus sphaericus strain SH19.
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  Label: Abstract
  Group: Ab
  Data: Lignite biosolubilization offers a mild route for valorizing low-rank coal, although the microbial processes that accompany solubilization remain incompletely defined. Here, an endogenous isolate designated Lysinibacillus sphaericus strain SH19 was evaluated using nitric-acid-pretreated Shengli lignite. Under the selected working conditions (4 M nitric-acid pretreatment, initial pH 8, 40&#176;C, and 16 days), the apparent solubilization rate reached 66.81%. Changes in A450, residual solid mass, culture pH, and extracellular protein concentration showed that chemical pretreatment and bacterial culture were both associated with the release of soluble lignite-derived material. SDS-PAGE and two-dimensional electrophoresis revealed treatment-associated differences in extracellular and intracellular protein patterns. LC-MS/MS analysis of excised protein spots yielded 85 candidate protein assignments; the revised supplementary table reports PEAKS scores, sequence coverage, peak area, and unique-peptide counts and highlights the limited support for several entries. GC-MS analysis produced 33 tentative library assignments in the solubilized fraction, but siloxane- and silyl-related signals were treated as possible analytical background, and no pathway was inferred from these assignments alone. Together, the data identify strain SH19 as a promising lignite-biosolubilizing isolate and provide candidate proteins and product signals for future validation. The proposed process model remains exploratory because direct enzyme assays, inhibitor experiments, carbon-balance measurements, transcriptomic or genetic validation, complete GC-MS blank subtraction, and authentic-standard confirmation were not available.&lt;br /&gt; (&#169; 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
– Name: Abstract
  Label: Competing Interests
  Group: Ab
  Data: Declarations. Conflict of interest: The authors declare no competing interests.
– Name: Ref
  Label: References
  Group: RefInfo
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– Name: GrantInfo
  Label: Grant Information
  Group: Grant
  Data: 2022QN02007 Natural Science Foundation of Inner Mongolia Autonomous Region; 2023QN02012 Natural Science Foundation of Inner Mongolia Autonomous Region
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: &lt;i&gt;Keywords: &lt;/i&gt;Lysinibacillus sphaericus strain SH19; GC–MS; LC–MS/MS; Lignite bioconversion; Lignite biosolubilization; Protein profiling
– Name: NumberCAS
  Label: Substance Nomenclature
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  Data: 0 (Bacterial Proteins)&lt;br /&gt;0 (Coal)
– Name: SubjectOther
  Label: SCR Organism
  Group: Su
  Data: Lysinibacillus sphaericus
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  Label: Entry Date(s)
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  Data: &lt;i&gt;Date Created: &lt;/i&gt;20260908 &lt;i&gt;Date Completed: &lt;/i&gt;20260908 &lt;i&gt;Latest Revision: &lt;/i&gt;20260908
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  Data: 20260909
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  Data: 10.1007/s00203-026-05162-4
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        Value: 10.1007/s00203-026-05162-4
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      – Code: eng
        Text: English
    Subjects:
      – SubjectFull: Gas Chromatography-Mass Spectrometry
        Type: general
      – SubjectFull: Solubility
        Type: general
      – SubjectFull: Tandem Mass Spectrometry
        Type: general
      – SubjectFull: Biotransformation
        Type: general
      – SubjectFull: Electrophoresis, Gel, Two-Dimensional
        Type: general
      – SubjectFull: Proteomics
        Type: general
      – SubjectFull: Bacillaceae metabolism
        Type: general
      – SubjectFull: Bacillaceae genetics
        Type: general
      – SubjectFull: Bacillaceae chemistry
        Type: general
      – SubjectFull: Bacillaceae isolation & purification
        Type: general
      – SubjectFull: Bacterial Proteins metabolism
        Type: general
      – SubjectFull: Bacterial Proteins chemistry
        Type: general
      – SubjectFull: Coal microbiology
        Type: general
      – SubjectFull: Coal analysis
        Type: general
    Titles:
      – TitleFull: Protein profiling and GC-MS product analysis provide insights into lignite solubilization and bioconversion by Lysinibacillus sphaericus strain SH19.
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            NameFull: He Z
      – PersonEntity:
          Name:
            NameFull: Li Q
      – PersonEntity:
          Name:
            NameFull: Yang Z
      – PersonEntity:
          Name:
            NameFull: Zhang S
      – PersonEntity:
          Name:
            NameFull: Liu Y
      – PersonEntity:
          Name:
            NameFull: Duan J
      – PersonEntity:
          Name:
            NameFull: Wang X
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 08
              M: 09
              Text: 2026 Sep 08
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 1432-072X
          Numbering:
            – Type: volume
              Value: 208
            – Type: issue
              Value: 12
          Titles:
            – TitleFull: Archives of microbiology
              Type: main
ResultId 1