Academic Journal
An Eco-Friendly N-Doped Carbon Quantum Dot-Based Fluorimetric Assay for Sensitive Betahistine Quantitation in Pharmaceuticals and Biological Fluids.
| Τίτλος: | An Eco-Friendly N-Doped Carbon Quantum Dot-Based Fluorimetric Assay for Sensitive Betahistine Quantitation in Pharmaceuticals and Biological Fluids. |
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| Συγγραφείς: | Kamel MA; Postgraduate Program in Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Cairo University, Cairo, Egypt.; Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 6th of October City, Egypt., Marzouk HM; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt., Michael AM; Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 6th of October City, Egypt.; Casual Academic, Charles Sturt University, Wagga Wagga, New South Wales, Australia., Abbas SS; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt., Nessim CK; Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 6th of October City, Egypt. |
| Πηγή: | Luminescence : the journal of biological and chemical luminescence [Luminescence] 2026 Sep; Vol. 41 (9), pp. e70617. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley & Sons Country of Publication: England NLM ID: 100889025 Publication Model: Print Cited Medium: Internet ISSN: 1522-7243 (Electronic) Linking ISSN: 15227235 NLM ISO Abbreviation: Luminescence Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Chichester, Sussex, UK : Wiley & Sons, c1999- |
| Ιατρικοί όροι (MeSH): | Carbon Quantum Dots*/chemistry , Fluorometry*/methods , Betahistine*/analysis , Betahistine*/urine , Nitrogen*/chemistry , Quantum Dots*/chemistry , Body Fluids*/chemistry, Pharmaceutical Preparations/chemistry ; Carbon/chemistry ; Humans ; Spectrometry, Fluorescence |
| Περίληψη: | A rapid, sensitive, and sustainable fluorimetric method based on fluorescence quenching of nitrogen-doped carbon quantum dots (N-CQDs) is introduced for the first time to determine betahistine dihydrochloride (BTH). The N-CQDs were prepared via a simple hydrothermal approach and characterized using transmission electron microscopy for electronic structure and morphology, ultraviolet-visible absorption spectroscopy, energy-dispersive X-ray spectroscopy, and Fourier-transform infrared spectroscopy, confirming their nanoscale structure, elemental composition, and surface functionalization. The N-CQDs gave strong blue emission at 440.0 nm upon excitation at 345.0 nm. BTH-induced concentration-dependent quenching of N-CQDs fluorescence was mainly due to static quenching. Under optimized conditions, the proposed method showed excellent linearity over the concentration range of 0.025-4.00 μg/mL. The method was successfully applied to pharmaceutical dosage forms, content uniformity testing, and urine samples, providing accurate results with satisfactory recoveries and good precision, without interference from common excipients or matrix components. Notably, the procedure operates entirely in distilled water, requiring minimal reagents with a short incubation time, highlighting its simplicity and practicality. The greenness and sustainability of the developed method were quantitatively evaluated and confirmed using the carbon footprint reduction index (CaFRI), the newly developed Nanomaterials Assessment Tool (NAT), and an integrated NQS-SAMI assessment. (© 2026 John Wiley & Sons Ltd.) |
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| Contributed Indexing: | Keywords: N‐doped carbon quantum dots; betahistine; fluorescence quenching; fluorimetry; sustainable analytical method |
| Substance Nomenclature: | X32KK4201D (Betahistine) N762921K75 (Nitrogen) 0 (Pharmaceutical Preparations) 7440-44-0 (Carbon) |
| Entry Date(s): | Date Created: 20260908 Date Completed: 20260908 Latest Revision: 20260914 |
| Update Code: | 20260914 |
| PubMed Central ID: | PMC13552260 |
| DOI: | 10.1002/bio.70617 |
| PMID: | 42708825 |
| Βάση Δεδομένων: | MEDLINE |
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