Academic Journal
Physicochemical and in vitro characterisation of antifungal PVA/chitosan composite wet-spun fibres loaded with hydroxypropyl-γ-cyclodextrin/amphotericin B complexes.
| Τίτλος: | Physicochemical and in vitro characterisation of antifungal PVA/chitosan composite wet-spun fibres loaded with hydroxypropyl-γ-cyclodextrin/amphotericin B complexes. |
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| Συγγραφείς: | Tasman RS; Department of Pharmaceutical Science and Technology, Faculty of Pharmacy, Universitas Hasanuddin, Makassar 90245, Indonesia., Muhtadi WK; Department of Pharmacy, Sekolah Tinggi Ilmu Farmasi (STIFAR) Riau, Pekanbaru 28293, Indonesia., Sari DI; Department of Pharmacy, Faculty of Mathematics and Natural Science, Universitas Lambung Mangkurat, Banjarmasin 70123, Indonesia., Aisyiah N; Department of Pharmaceutical Science and Technology, Faculty of Pharmacy, Universitas Hasanuddin, Makassar 90245, Indonesia., Khumaera AA; Department of Pharmaceutical Science and Technology, Faculty of Pharmacy, Universitas Hasanuddin, Makassar 90245, Indonesia., Mansur YS; Department of Pharmaceutical Science and Technology, Faculty of Pharmacy, Universitas Hasanuddin, Makassar 90245, Indonesia., Hardiyanti AM; Department of Pharmaceutical Science and Technology, Faculty of Pharmacy, Universitas Hasanuddin, Makassar 90245, Indonesia., Saputra MD; Department of Pharmaceutical Science and Technology, Faculty of Pharmacy, Universitas Hasanuddin, Makassar 90245, Indonesia., Sartini; Department of Pharmaceutical Science and Technology, Faculty of Pharmacy, Universitas Hasanuddin, Makassar 90245, Indonesia., Himawan A; Department of Pharmaceutical Science and Technology, Faculty of Pharmacy, Universitas Hasanuddin, Makassar 90245, Indonesia. Electronic address: himawan@unhas.ac.id. |
| Πηγή: | International journal of pharmaceutics [Int J Pharm] 2026 Oct 05; Vol. 703, pp. 127372. Date of Electronic Publication: 2026 Sep 05. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7804127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3476 (Electronic) Linking ISSN: 03785173 NLM ISO Abbreviation: Int J Pharm Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| Ιατρικοί όροι (MeSH): | Chitosan*/chemistry , Chitosan*/administration & dosage , Antifungal Agents*/chemistry , Antifungal Agents*/administration & dosage , Antifungal Agents*/pharmacology , Amphotericin B*/chemistry , Amphotericin B*/administration & dosage , Amphotericin B*/pharmacology , Polyvinyl Alcohol*/chemistry , Polyvinyl Alcohol*/administration & dosage , gamma-Cyclodextrins*/chemistry , gamma-Cyclodextrins*/administration & dosage, Candida albicans/drug effects ; Candida albicans/growth & development ; Delayed-Action Preparations/chemistry ; Drug Carriers/chemistry ; Hemolysis/drug effects ; Solubility ; Microbial Sensitivity Tests ; Drug Liberation |
| Περίληψη: | Chronic wounds infected by Candida albicans impose a significant clinical burden, yet topical amphotericin B (AMB) delivery is constrained by low aqueous solubility. Wet-spun poly(vinyl alcohol) (PVA)/chitosan fibres loaded with an AMB/hydroxypropyl-γ-cyclodextrin (HP-γ-CD) inclusion complex were developed as a sustained-release antifungal wound dressing. Phase solubility analysis confirmed inclusion complex formation with an apparent stability constant of 36.1 × 103 M⁻1 in distilled water (AL-type profile, R2 = 0.9984), characterised by Fourier-transform infrared spectroscopy (FTIR), 1H NMR, and scanning electron microscopy (SEM). IC-SE-1 achieved ∼ 354-fold and ∼ 136-fold solubility enhancement in distilled water and phosphate-buffered saline (PBS, pH 7.4), respectively, while fully retaining antifungal activity (MIC = 1 µg/mL; p = 0.112 vs. pure AMB). The complex was incorporated into fibres at four PVA:chitosan ratios and crosslinked with tripolyphosphate and glutaraldehyde. Formulation P6C4 (PVA 60%:chitosan 40%) demonstrated the most favourable overall performance, with the highest water-holding capacity (346.51 ± 4.10%), swelling (463.40 ± 11.12%), tensile strength (18.47 ± 2.18 MPa), and elongation (431.57 ± 52.22%). Woven fibres maintained inhibition zones against C. albicans from day 2 to day 7, with AMB release exceeding the MIC throughout, while haemolysis remained below 5% (ASTM F756-08). HP-γ-CD complexation enables sustained above-MIC AMB delivery from a dual-crosslinked biopolymeric dressing, providing in vitro proof-of-concept for Candida-infected chronic wounds, pending cytotoxicity and in vivo validation. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Amphotericin B; Antifungal; Cyclodextrin; Inclusion complex; PVA/chitosan; Wet Spinning |
| Substance Nomenclature: | 9012-76-4 (Chitosan) 0 (Antifungal Agents) 7XU7A7DROE (Amphotericin B) 9002-89-5 (Polyvinyl Alcohol) 0 (gamma-Cyclodextrins) 0 (hydroxypropyl-gamma-cyclodextrin) 0 (Delayed-Action Preparations) 0 (Drug Carriers) |
| Entry Date(s): | Date Created: 20260905 Date Completed: 20260919 Latest Revision: 20260919 |
| Update Code: | 20260920 |
| DOI: | 10.1016/j.ijpharm.2026.127372 |
| PMID: | 42700906 |
| Βάση Δεδομένων: | MEDLINE |
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