Academic Journal
Intranasal immunization in mice with spore-associated serine-aspartate repeat-containing protein D from Staphylococcus aureus secreted in Bacillus subtilis.
| Τίτλος: | Intranasal immunization in mice with spore-associated serine-aspartate repeat-containing protein D from Staphylococcus aureus secreted in Bacillus subtilis. |
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| Συγγραφείς: | Vo HTT; Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam.; Faculty of Natural Sciences, Quy Nhon University, Quy Nhon, Vietnam., Pham VK; Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam., Ngo KVT; Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam., Nguyen TTN; Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam., Nguyen NNY; Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam., Nguyen TMD; Faculty of Natural Sciences, Quy Nhon University, Quy Nhon, Vietnam., Nguyen HD; Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City, Vietnam. ndhoang@hcmus.edu.vn.; Vietnam National University, Ho Chi Minh City, Vietnam. ndhoang@hcmus.edu.vn. |
| Πηγή: | Biotechnology letters [Biotechnol Lett] 2026 Sep 04; Vol. 48 (5). Date of Electronic Publication: 2026 Sep 04. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-6776 (Electronic) Linking ISSN: 01415492 NLM ISO Abbreviation: Biotechnol Lett Subsets: MEDLINE |
| Imprint Name(s): | Publication: 1999- : Dordrecht : Kluwer Academic Publishers Original Publication: [Kew, Eng., Science and Technology Letters] |
| Ιατρικοί όροι (MeSH): | Bacillus subtilis*/genetics , Bacillus subtilis*/metabolism , Staphylococcus aureus*/genetics , Staphylococcus aureus*/immunology , Bacterial Proteins*/genetics , Bacterial Proteins*/immunology , Adhesins, Bacterial*/genetics , Adhesins, Bacterial*/immunology , Staphylococcal Vaccines*/immunology , Staphylococcal Vaccines*/administration & dosage , Staphylococcal Vaccines*/genetics, Recombinant Proteins/genetics ; Recombinant Proteins/immunology ; Recombinant Proteins/metabolism ; Spores, Bacterial/genetics ; Spores, Bacterial/immunology ; Antibodies, Bacterial/blood ; Immunoglobulin G/blood ; Immunization/methods ; Animals ; Administration, Intranasal ; Mice ; Mice, Inbred BALB C ; Protein Subunit Vaccines ; Female |
| Περίληψη: | Staphylococcus aureus is a major human pathogen causing diseases ranging from mild skin infections to life-threatening conditions due to its diverse virulence factors. The emergence of antibiotic-resistant strains underscores the urgent need for an effective vaccine, though none has yet achieved clinical success. The nasal mucosa serves as a natural reservoir in about 30% of individuals and represents a key source of subsequent infections, particularly in immunocompromised patients. Among its virulence factors, SdrD, a surface-anchored MSCRAMM adhesin associated with methicillin-resistant strains (MRSA), promotes adherence to squamous epithelial cells by binding to desmoglein 1, facilitating epithelial attachment, evasion of mucociliary clearance, and subsequent invasion. Bacillus subtilis, an endotoxin-free organism recognized for its safety, has been extensively developed as a platform for secreted recombinant protein production. This study aimed to utilize the host strain B. subtilis to express and secrete recombinant SdrD antigen, using the control of a strong IPTG-inducible Pgrac212 promoter in a pHT expression vector for B. subtilis. Following IPTG induction, extracellular SdrD was analyzed by SDS-PAGE and confirmed by Western blotting using anti-SdrD polyclonal antibodies. The intranasal administration of concentrated secretions, combined with B. subtilis spores as adjuvants, to mice elicited significant antigen-specific IgA and IgG responses, providing both mucosal and systemic immunity. The co-administration with spores enhanced both the magnitude and persistence of antibody responses compared to antigen alone. These findings demonstrate that B. subtilis can serve as an effective platform for recombinant protein secretion and mucosal antigen delivery, supporting its application in vaccine development against S. aureus infection via mucosal route. (© 2026. The Author(s), under exclusive licence to Springer Nature B.V.) |
| Competing Interests: | Declarations. Conflict of interest: The authors do not have any conflict of interest to declare. Ethical approval: This study was approved by the Animal Care and Use Committee, University of Science (ACUCUS) at Ho Chi Minh City, signed on March 6th, 2024. |
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| Grant Information: | 108.06-2019.11 National Foundation for Science and Technology Development |
| Contributed Indexing: | Keywords: Bacillus subtilis; Staphylococcus aureus; Pgrac212; SdrD; Spores |
| Substance Nomenclature: | 0 (Recombinant Proteins) 0 (Bacterial Proteins) 0 (Adhesins, Bacterial) 0 (Antibodies, Bacterial) 0 (Protein Subunit Vaccines) 0 (Immunoglobulin G) 0 (Staphylococcal Vaccines) |
| Entry Date(s): | Date Created: 20260904 Date Completed: 20260904 Latest Revision: 20260904 |
| Update Code: | 20260905 |
| DOI: | 10.1007/s10529-026-03782-w |
| PMID: | 42696091 |
| Βάση Δεδομένων: | MEDLINE |
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