Academic Journal
Exosomal miR-22-3p targets p53 to regulate endometrial epithelial cell function in the pathogenesis of endometriosis.
| Τίτλος: | Exosomal miR-22-3p targets p53 to regulate endometrial epithelial cell function in the pathogenesis of endometriosis. |
|---|---|
| Συγγραφείς: | Yu C; Department of Obstetrics and Gynecology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010050, Inner Mongolia, China., Li Y; Department of Obstetrics and Gynecology, Inner Mongolia Maternal and Child Health Care Hospital, Hohhot, 010020, Inner Mongolia, China. 287185898@qq.com., Qi G; Inner Mongolia Medical University, Hohhot, 010050, Inner Mongolia, China., Qiao Q; Department of Obstetrics and Gynecology, Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010050, Inner Mongolia, China. qiaoqiao771011@126.com. |
| Πηγή: | Journal of molecular histology [J Mol Histol] 2026 Sep 04; Vol. 57 (5). Date of Electronic Publication: 2026 Sep 04. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer Netherlands Country of Publication: Netherlands NLM ID: 101193653 Publication Model: Electronic Cited Medium: Internet ISSN: 1567-2387 (Electronic) Linking ISSN: 15672379 NLM ISO Abbreviation: J Mol Histol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Dordrecht, The Netherlands : Springer Netherlands Original Publication: Dordrecht, The Netherlands : Kluwer Academic Publishers, 2004- |
| Ιατρικοί όροι (MeSH): | MicroRNAs*/genetics , MicroRNAs*/metabolism , Endometriosis*/genetics , Endometriosis*/pathology , Endometriosis*/metabolism , Exosomes*/metabolism , Exosomes*/genetics , Tumor Suppressor Protein p53*/metabolism , Tumor Suppressor Protein p53*/genetics , Epithelial Cells*/metabolism , Epithelial Cells*/pathology , Endometrium*/pathology , Endometrium*/metabolism, Cell Proliferation/genetics ; Cell Movement/genetics ; Apoptosis/genetics ; Humans ; Female ; Adult |
| Περίληψη: | Endometriosis (EMs) is a widespread gynecological disease, affecting approximately 6-10% of females of child-bearing age. Despite its significant influence on women's health, the specific pathophysiology remains unclear. The enrichment of miR-22-3p in exosomes has been implicated in cellular proliferation and migration. This study aimed to elucidate the mechanisms by which exosomal miR-22-3p affects endometrial epithelial cell function in the etiology of endometriosis. The patient samples and clinical data were acquired from the Affiliate Hospital of Inner Mongolia Medical University. Quantitative reverse transcription‒polymerase chain reaction (qRT‒PCR), a miRNA microarray and Western blotting were used to confirm that the level of miR-22-3p was specifically elevated in peripheral blood exosomes from EMs patients. The influence of miR-22-3p on EMs-derived exosomes was determined by Transwell and cell counting kit-8 (CCK-8) assays. Luciferase reporter assays, RNA pull-down assays, and fluorescence in situ hybridization (FISH) were performed to determine whether exosomal miR-22-3p binds to p53. miR-22-3p expression was significantly elevated in peripheral blood exosomes (p < 0.001) from patients with endometriosis. ROC curve analysis demonstrated the high sensitivity (93.55%) and specificity (84.85%) of this correlation, suggesting that miR-22-3p is a promising diagnostic biomarker. Endometrial epithelial cell proliferation, migration, and invasion are regulated by exosomal miR-22-3p, which targets and inhibits p53, a crucial tumor suppressor related to cell cycle regulation and apoptosis. Exosomal miR-22-3p regulates endometrial epithelial cell proliferation, migration, and invasion by targeting and inhibiting p53. Combining standard endometriosis treatments with exosome-mediated miR-22-3p targeting of p53 could restore p53 function, induce apoptosis, and reduce disease recurrence, providing a promising therapeutic strategy for endometriosis management. (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Conflict of interest: The authors declare no conflict of interest. Ethical approval: This study was approved by the Ethics Committee of the Affiliated Hospital of Inner Mongolia Medical University (Approval No. FS(KY2023092). Written informed consent was obtained from all participants prior to enrollment. All procedures were conducted in accordance with the Declaration of Helsinki. |
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| Grant Information: | 2022QN0826 Natural Science Foundation of the Inner Mongolia Autonomous Region; 20242024GLLH0281 Science and Technology Program of the Joint Fund of Scientific Research for the Public Hospitals of Inner Mongolia Academy of Medical Sciences |
| Contributed Indexing: | Keywords: Apoptosis; Endometrial epithelial cells; Endometriosis; Exosomes; MiR-22-3p; P53 |
| Substance Nomenclature: | 0 (MicroRNAs) 0 (Tumor Suppressor Protein p53) 0 (MIRN22 microRNA, human) |
| Entry Date(s): | Date Created: 20260904 Date Completed: 20260904 Latest Revision: 20260906 |
| Update Code: | 20260906 |
| PubMed Central ID: | PMC13541819 |
| DOI: | 10.1007/s10735-026-10966-1 |
| PMID: | 42693330 |
| Βάση Δεδομένων: | MEDLINE |
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