Academic Journal
Characterization of yam virus X isolates from Dioscorea trifida in Brazil.
| Τίτλος: | Characterization of yam virus X isolates from Dioscorea trifida in Brazil. |
|---|---|
| Συγγραφείς: | Araújo CPFS; Plant Virus Department, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstrasse 7B, 38124, Brunswick, Germany., Knierim D; Plant Virus Department, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstrasse 7B, 38124, Brunswick, Germany., de Oliveira SAS; Embrapa Mandioca e Fruticultura, Rua Embrapa S/nº, Chapadinha, Bahia, 44380-000, Cruz das Almas, Brazil., Blawid R; Department of Agronomy, Federal Rural University of Pernambuco, Rua Dom Manuel de Medeiros S/nº, Dois Irmãos, Pernambuco, 52171-900, Recife, Brazil., Margaria P; Plant Virus Department, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstrasse 7B, 38124, Brunswick, Germany. paolo.margaria@dsmz.de. |
| Πηγή: | BMC research notes [BMC Res Notes] 2026 Sep 02; Vol. 19 (1). Date of Electronic Publication: 2026 Sep 02. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Biomed Central Country of Publication: England NLM ID: 101462768 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-0500 (Electronic) Linking ISSN: 17560500 NLM ISO Abbreviation: BMC Res Notes Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Biomed Central, 2008. |
| Ιατρικοί όροι (MeSH): | Dioscorea*/virology , Potexvirus*/genetics , Potexvirus*/isolation & purification , Potexvirus*/classification , Plant Diseases*/virology , Genome, Viral*, RNA, Viral/genetics ; Brazil ; Phylogeny ; Genetic Variation ; High-Throughput Nucleotide Sequencing |
| Περίληψη: | Objective: Yam virus X (YVX; Potexvirus ecsdioscoreae) is a positive-sense, flexuous RNA virus belonging to the family Alphaflexiviridae. It has been first reported from Guadeloupe, a French archipelago located in the Caribbean Sea. In this study, we investigated the virome in yam (Dioscorea spp.) plant material collected in the state of Bahia (Brazil) by high-throughput sequencing (HTS) on Illumina platform. The objective of the investigation was to explore the occurrence of YVX in yam from South America, and to study its genetic diversity compared to the only one YVX genome sequence available in the GenBank public database. Results: An initial investigation by HTS of bulked RNA extracts (n=23, combined into 4 pools) revealed occurrence of YVX only in samples collected in the region of Valença. Subsequent screening by RT-PCR of the individual samples composing the pool uncovered infection with YVX only in Discorea trifida. Total RNA extracts from three infected plants were individually sequenced, resulting in the assembly of three complete genome sequences of YVX, showing ~84% nucleotide identity to the reference sequence from Guadeloupe. Our results contribute to expanding the pool of sequences available for YVX, supporting detection purposes and stimulating additional investigations for future studies on YVX diversity and evolution. (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. |
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| Contributed Indexing: | Keywords: Bioinformatics; Illumina sequencing; Plant virus; Potexvirus; South America |
| Substance Nomenclature: | 0 (RNA, Viral) |
| Entry Date(s): | Date Created: 20260903 Date Completed: 20260903 Latest Revision: 20260905 |
| Update Code: | 20260905 |
| PubMed Central ID: | PMC13540820 |
| DOI: | 10.1186/s13104-026-07987-0 |
| PMID: | 42687209 |
| Βάση Δεδομένων: | MEDLINE |
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