Academic Journal
View in EDS

A novel urease-producing strain effectively induces cadmium biomineralization under low-temperature stress.

Bibliographic Details
Title: A novel urease-producing strain effectively induces cadmium biomineralization under low-temperature stress.
Authors: Yu Y; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China., Zheng Q; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China., Peng F; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China., Huang Y; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China., Qiu C; Agricultural and Livestock Products Engineering Technology Research Center of Xizang Autonomous Region, Institute of Agricultural Quality Standard and Testing, Xizang Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850032, China., Liu Q; Agricultural and Livestock Products Engineering Technology Research Center of Xizang Autonomous Region, Institute of Agricultural Quality Standard and Testing, Xizang Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850032, China., Liang L; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China., Wu B; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China. Electronic address: wub@cdut.edu.cn.
Source: Journal of environmental sciences (China) [J Environ Sci (China)] 2026 Oct; Vol. 168, pp. 187-195. Date of Electronic Publication: 2026 Jan 14.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: IOS Press Country of Publication: Netherlands NLM ID: 100967627 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1001-0742 (Print) Linking ISSN: 10010742 NLM ISO Abbreviation: J Environ Sci (China) Subsets: MEDLINE
Imprint Name(s): Publication: Amsterdam : IOS Press
Original Publication: Beijing : Editorial Dept. of Journal of Environmental Sciences (China), 1989-
MeSH Terms: Cadmium*/metabolism , Soil Pollutants*/metabolism , Pseudomonas*/metabolism , Urease*/metabolism, Biomineralization ; Cold Temperature ; Biodegradation, Environmental
Abstract: Microbially induced carbonate precipitation (MICP) has been widely used to immobilize Cadmium (Cd) in contaminated soils in mining-affected regions. However, its remediation efficacy under low-temperature stress, as well as the nucleation process that regulates Cd biomineralization via carbonate precipitation by psychrophilic bacteria, has yet to be investigated. Here, we isolated Pseudomonas sp. J-6, a novel urease-producing strain from tailings in high-altitude cold regions, exhibiting unparalleled cold adaptability at 5 °C and achieving 95.85 % Cd removal efficiency by MICP at 10 °C. Furthermore, the coprecipitation process of Ca1-xCdxCO3 was clarified through the continuous observation of the precipitates after the low-temperature MICP reaction. The crystal morphology transitioned from loose vaterite in the early stage to a dense square-block morphology in the middle stage. Cd2+ progressively shifted from a surface-bound state to lattice incorporation, ultimately resulting in the formation of stable Cd-substituted calcite crystals. In this process, low temperatures led to the formation of larger, highly ordered Cd-substituted calcite crystals, thereby strengthening Cd sequestration and its long-term stability. In addition, under low-temperature stress, Pseudomonas sp. J-6 induced MICP reaction decreased the bioavailable Cd in alpine slag soil by 44.85 % and enhanced physical properties. In the freeze-thaw cycles, the remediation efficiency remained stable. This study clarified the biomineralization potential in high-altitude cryogenic environments and the nucleation process of Cd biomineralization by psychrophilic bacteria-induced carbonate precipitation, filling a critical research gap in its application under extreme conditions and highlighting its promise for sustainable remediation of heavy metal pollution under low-temperature stress.
(Copyright © 2026. Published by Elsevier B.V.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Cadmium; Coprecipitation process; Low-temperature stress; Microbially induced carbonate precipitation (MICP); Soil remediation
Substance Nomenclature: 00BH33GNGH (Cadmium)
0 (Soil Pollutants)
EC 3.5.1.5 (Urease)
Entry Date(s): Date Created: 20260901 Date Completed: 20260901 Latest Revision: 20260901
Update Code: 20260902
DOI: 10.1016/j.jes.2026.01.026
PMID: 42680376
Database: MEDLINE
FullText Text:
  Availability: 0
CustomLinks:
  – Url: https://www.doi.org/10.1016/j.jes.2026.01.026?
    Name: ScienceDirect (all content) (s7799221)
    Category: fullText
    Text: View record from ScienceDirect
    MouseOverText: View record from ScienceDirect
  – Url: https://resolver.ebsco.com/c/fiv2js/result?sid=EBSCO:cmedm&genre=article&issn=10010742&ISBN=&volume=168&issue=&date=20261001&spage=187&pages=187-195&title=Journal of environmental sciences (China)&atitle=A%20novel%20urease-producing%20strain%20effectively%20induces%20cadmium%20biomineralization%20under%20low-temperature%20stress.&aulast=Yu%20Y&id=DOI:10.1016/j.jes.2026.01.026
    Name: Full Text Finder (for New FTF UI) (ns324271)
    Category: fullText
    Text: Full Text Finder
    MouseOverText: Full Text Finder
Header DbId: cmedm
DbLabel: MEDLINE
An: 42680376
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: A novel urease-producing strain effectively induces cadmium biomineralization under low-temperature stress.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Yu+Y%22">Yu Y</searchLink>; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China.<br /><searchLink fieldCode="AU" term="%22Zheng+Q%22">Zheng Q</searchLink>; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China.<br /><searchLink fieldCode="AU" term="%22Peng+F%22">Peng F</searchLink>; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China.<br /><searchLink fieldCode="AU" term="%22Huang+Y%22">Huang Y</searchLink>; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China.<br /><searchLink fieldCode="AU" term="%22Qiu+C%22">Qiu C</searchLink>; Agricultural and Livestock Products Engineering Technology Research Center of Xizang Autonomous Region, Institute of Agricultural Quality Standard and Testing, Xizang Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850032, China.<br /><searchLink fieldCode="AU" term="%22Liu+Q%22">Liu Q</searchLink>; Agricultural and Livestock Products Engineering Technology Research Center of Xizang Autonomous Region, Institute of Agricultural Quality Standard and Testing, Xizang Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850032, China.<br /><searchLink fieldCode="AU" term="%22Liang+L%22">Liang L</searchLink>; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China.<br /><searchLink fieldCode="AU" term="%22Wu+B%22">Wu B</searchLink>; State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China. Electronic address: wub@cdut.edu.cn.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22100967627%22">Journal of environmental sciences (China)</searchLink> [J Environ Sci (China)] 2026 Oct; Vol. 168, pp. 187-195. <i>Date of Electronic Publication: </i>2026 Jan 14.
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22IOS+Press%22">IOS Press </searchLink><i>Country of Publication: </i>Netherlands <i>NLM ID: </i>100967627 <i>Publication Model: </i>Print-Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1001-0742 (Print) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%2210010742%22">10010742 </searchLink><i>NLM ISO Abbreviation: </i>J Environ Sci (China) <i>Subsets: </i>MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Publication</i>: Amsterdam : IOS Press<br /><i>Original Publication</i>: Beijing : Editorial Dept. of Journal of Environmental Sciences (China), 1989-
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Cadmium%22">Cadmium*</searchLink>/<searchLink fieldCode="MM" term="%22Cadmium+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Soil+Pollutants%22">Soil Pollutants*</searchLink>/<searchLink fieldCode="MM" term="%22Soil+Pollutants+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Pseudomonas%22">Pseudomonas*</searchLink>/<searchLink fieldCode="MM" term="%22Pseudomonas+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Urease%22">Urease*</searchLink>/<searchLink fieldCode="MM" term="%22Urease+metabolism%22">metabolism</searchLink><br /><searchLink fieldCode="MH" term="%22Biomineralization%22">Biomineralization</searchLink> ; <searchLink fieldCode="MH" term="%22Cold+Temperature%22">Cold Temperature</searchLink> ; <searchLink fieldCode="MH" term="%22Biodegradation%2C+Environmental%22">Biodegradation, Environmental</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Microbially induced carbonate precipitation (MICP) has been widely used to immobilize Cadmium (Cd) in contaminated soils in mining-affected regions. However, its remediation efficacy under low-temperature stress, as well as the nucleation process that regulates Cd biomineralization via carbonate precipitation by psychrophilic bacteria, has yet to be investigated. Here, we isolated Pseudomonas sp. J-6, a novel urease-producing strain from tailings in high-altitude cold regions, exhibiting unparalleled cold adaptability at 5 °C and achieving 95.85 % Cd removal efficiency by MICP at 10 °C. Furthermore, the coprecipitation process of Ca<subscript>1-</subscript><subscript>x</subscript>Cd<subscript>x</subscript>CO<subscript>3</subscript> was clarified through the continuous observation of the precipitates after the low-temperature MICP reaction. The crystal morphology transitioned from loose vaterite in the early stage to a dense square-block morphology in the middle stage. Cd<superscript>2+</superscript> progressively shifted from a surface-bound state to lattice incorporation, ultimately resulting in the formation of stable Cd-substituted calcite crystals. In this process, low temperatures led to the formation of larger, highly ordered Cd-substituted calcite crystals, thereby strengthening Cd sequestration and its long-term stability. In addition, under low-temperature stress, Pseudomonas sp. J-6 induced MICP reaction decreased the bioavailable Cd in alpine slag soil by 44.85 % and enhanced physical properties. In the freeze-thaw cycles, the remediation efficiency remained stable. This study clarified the biomineralization potential in high-altitude cryogenic environments and the nucleation process of Cd biomineralization by psychrophilic bacteria-induced carbonate precipitation, filling a critical research gap in its application under extreme conditions and highlighting its promise for sustainable remediation of heavy metal pollution under low-temperature stress.<br /> (Copyright © 2026. Published by Elsevier B.V.)
– Name: Abstract
  Label: Competing Interests
  Group: Ab
  Data: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
– Name: SubjectMinor
  Label: Contributed Indexing
  Group:
  Data: <i>Keywords: </i>Cadmium; Coprecipitation process; Low-temperature stress; Microbially induced carbonate precipitation (MICP); Soil remediation
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: 00BH33GNGH (Cadmium)<br />0 (Soil Pollutants)<br />EC 3.5.1.5 (Urease)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: <i>Date Created: </i>20260901 <i>Date Completed: </i>20260901 <i>Latest Revision: </i>20260901
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20260902
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1016/j.jes.2026.01.026
– Name: AN
  Label: PMID
  Group: ID
  Data: 42680376
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42680376
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.jes.2026.01.026
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 187
    Subjects:
      – SubjectFull: Biomineralization
        Type: general
      – SubjectFull: Cold Temperature
        Type: general
      – SubjectFull: Biodegradation, Environmental
        Type: general
      – SubjectFull: Cadmium metabolism
        Type: general
      – SubjectFull: Soil Pollutants metabolism
        Type: general
      – SubjectFull: Pseudomonas metabolism
        Type: general
      – SubjectFull: Urease metabolism
        Type: general
    Titles:
      – TitleFull: A novel urease-producing strain effectively induces cadmium biomineralization under low-temperature stress.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Yu Y
      – PersonEntity:
          Name:
            NameFull: Zheng Q
      – PersonEntity:
          Name:
            NameFull: Peng F
      – PersonEntity:
          Name:
            NameFull: Huang Y
      – PersonEntity:
          Name:
            NameFull: Qiu C
      – PersonEntity:
          Name:
            NameFull: Liu Q
      – PersonEntity:
          Name:
            NameFull: Liang L
      – PersonEntity:
          Name:
            NameFull: Wu B
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 10
              Text: 2026 Oct
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-print
              Value: 1001-0742
          Numbering:
            – Type: volume
              Value: 168
          Titles:
            – TitleFull: Journal of environmental sciences (China)
              Type: main
ResultId 1