Academic Journal
Organ-specific transcriptional programs regulate cellular plasticity in lymphatic endothelial cells.
| Τίτλος: | Organ-specific transcriptional programs regulate cellular plasticity in lymphatic endothelial cells. |
|---|---|
| Συγγραφείς: | Tsuyama Y; Department of Oral and Maxillofacial Surgical Oncology, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Japan; Department of Biochemistry, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Japan., Takahashi K; Department of Biochemistry, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Japan., Kobayashi M; Department of Biochemistry, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Japan. Electronic address: kobayashi.m.0fde@m.isct.ac.jp., Benzemam Suzuki L; Department of Biochemistry, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Japan., Akagi H; Department of Biochemistry, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Japan., Yokozawa M; Department of Biochemistry, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Japan; Department of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University, Japan., Imafuku T; Department of Molecular Pathophysiology, Wakayama Medical University, Japan., Hashimoto S; Department of Molecular Pathophysiology, Wakayama Medical University, Japan., Matsunaga Y; Institute of Industrial Science, The University of Tokyo, Japan., Itoh F; Institute of Liberal Arts and Sciences, University of Toyama, Japan., Harada H; Department of Oral and Maxillofacial Surgical Oncology, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Japan., Hong YK; Division of Surgical Sciences, Department of Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, USA., Watabe T; Department of Biochemistry, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Japan; Oral Science Center, Institute of Biomedical Engineering, Institute of Science Tokyo, Japan. Electronic address: t-watabe.bch@tmd.ac.jp. |
| Πηγή: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2026 Oct 08; Vol. 834, pp. 154315. Date of Electronic Publication: 2026 Jul 20. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| Imprint Name(s): | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| Ιατρικοί όροι (MeSH): | Endothelial Cells*/metabolism , Endothelial Cells*/cytology , Cell Plasticity*/genetics , Lymphatic Vessels*/cytology , Lymphatic Vessels*/metabolism , Transcription, Genetic*, Lung/cytology ; Lung/metabolism ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Transforming Growth Factor beta/metabolism ; Myocardium/cytology ; Myocardium/metabolism ; Tumor Suppressor Proteins/genetics ; Tumor Suppressor Proteins/metabolism ; Animals ; Mice ; Endothelial-Mesenchymal Transition ; Prospero-Related Homeobox 1 Protein ; Organ Specificity ; Cells, Cultured ; Signal Transduction ; Gene Expression Profiling |
| Περίληψη: | Lymphatic vessels play essential roles in maintaining fluid homeostasis and responding to inflammatory and tissue environmental changes. During these biological processes, lymphatic endothelial cells (LECs) lining lymphatic vessels adapt to external stimuli in various organs and alter their characteristics. While organ-specific molecular and functional heterogeneity has been well-characterized in blood vascular endothelial cells, the extent and functional significance of such heterogeneity in LECs remain poorly understood. In this study, we established LECs derived from the heart and lung tissues of Prox1-GFP mice for comparative analyses. Both cell types retained lymphatic endothelial marker expression and exhibited tube-forming capacity in two- and three-dimensional culture systems, indicating the preservation of functional LEC phenotypes. Transcriptomic analysis revealed that LEC gene expression profiles were primarily segregated according to the organ of origin, indicating that LECs possess stable organ-specific transcriptional states. Notably, cardiac-derived LECs displayed enrichment of proliferation-related pathways, including MYC signaling, as well as inflammatory pathways such as TNF-α-NFκB and IL2-STAT5 signaling, indicating a transcriptional state primed for enhanced responsiveness to external stimuli. Furthermore, cardiac-derived LECs exhibited increased susceptibility to TGF-β-induced endothelial-to-mesenchymal transition (EndoMT) compared with pulmonary-derived LECs, reflecting enhanced cellular plasticity. These findings demonstrate that organ-specific transcriptional states regulate stimulus responsiveness, thereby defining cellular plasticity in LECs. (Copyright © 2026. Published by Elsevier Inc.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Endothelial-to-mesenchymal transition; Lymphatic endothelial cell; Organ-specific transcriptional program; Transforming growth factor-β |
| Substance Nomenclature: | 0 (Prospero-Related Homeobox 1 Protein) 0 (Homeodomain Proteins) 0 (Transforming Growth Factor beta) 0 (Tumor Suppressor Proteins) |
| Entry Date(s): | Date Created: 20260828 Date Completed: 20260909 Latest Revision: 20260909 |
| Update Code: | 20260910 |
| DOI: | 10.1016/j.bbrc.2026.154315 |
| PMID: | 42664823 |
| Βάση Δεδομένων: | MEDLINE |
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