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Mating-Induced Behavioral Changes in Female Drosophila Are Modulated by Serotonin.

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Τίτλος: Mating-Induced Behavioral Changes in Female Drosophila Are Modulated by Serotonin.
Συγγραφείς: Guerra-Ayala S; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile., Cortés-Henott A; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile., Amado-Hinojosa P; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile., González-Teuber M; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile., Gajardo I; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile., Campusano JM; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.
Πηγή: International journal of molecular sciences [Int J Mol Sci] 2026 Aug 07; Vol. 27 (16). Date of Electronic Publication: 2026 Aug 07.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
Imprint Name(s): Original Publication: Basel, Switzerland : MDPI, [2000-
Ιατρικοί όροι (MeSH): Serotonin*/metabolism , Sexual Behavior, Animal*/physiology , Drosophila melanogaster*/physiology, Brain/metabolism ; Serotonergic Neurons/metabolism ; Animals ; Female ; Odorants ; Social Behavior ; Behavior, Animal
Περίληψη: Mating influences the physiology of female animals and induces changes in behaviors associated with securing resources for their offspring. Modulatory neurotransmitters are known to adjust behavioral responses of animals according to environmental cues and internal physiological states. In that regard, it has been shown that serotonin (5-HT) modulates some post-mating behavioral features in Drosophila. However, it remains unknown whether serotonin regulates post-mating social behavior or responses to food cues in flies, which are among several behaviors that serve to secure their survival and reproduction. Here, we report a behavioral paradigm, the yeast-response test (YRT), for exploring locomotor and social behavioral changes in groups of virgin and mated females using yeast odor as a naturalistic food signal. Using this setup, we found that exposure to yeast odor increases social interactions in mated but not virgin females under non-aversive conditions and that these responses are suppressed at aversive temperatures. Furthermore, chronic impairment of serotonin transporter function reverses the social behavioral response to food odor in mated females, without affecting their olfactory capacity. Whole-brain 5-HT content, as well as histone serotonylation, were unchanged between virgin and mated females, suggesting that 5-HT contribution to these changes is mediated at a circuit level rather than by global neuromodulatory mechanisms. Consistent with this idea, silencing serotonin projection neurons (SPNs) selectively abolished mating-induced changes in social behavior without affecting the locomotor effects. These results provide further support for data in the literature showing that SPNs belong to a discrete circuit underlying social behaviors in female flies. Together, these results support a model in which serotonergic signaling shapes post-mating behavior in Drosophila through functionally segregated neural circuits.
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Curr Biol. 2010 Jun 8;20(11):1000-5. (PMID: 20471268)
Sci Signal. 2013 Jul 23;6(285):ra61. (PMID: 23882120)
J Neurogenet. 2012 Sep;26(3-4):306-16. (PMID: 23106154)
Front Behav Neurosci. 2019 Jan 09;12:334. (PMID: 30687036)
Nature. 2019 Mar;567(7749):535-539. (PMID: 30867594)
PLoS One. 2023 Mar 23;18(3):e0275795. (PMID: 36952449)
Endocrinology. 2010 Aug;151(8):3805-14. (PMID: 20484459)
Eur J Med Res. 2022 Oct 17;27(1):203. (PMID: 36253869)
PLoS One. 2018 Jun 8;13(6):e0198702. (PMID: 29883493)
Funct Ecol. 2018 Nov;32(11):2542-2552. (PMID: 31007331)
J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2023 Jul;209(4):467-488. (PMID: 36658447)
Neuron. 2018 Apr 18;98(2):350-365.e5. (PMID: 29673482)
Neurochem Int. 2020 Sep;138:104753. (PMID: 32416114)
Cells. 2022 May 05;11(9):. (PMID: 35563850)
Epigenetics Chromatin. 2026 Jan 04;19(1):9. (PMID: 41486372)
Neuron. 2012 Oct 4;76(1):1-11. (PMID: 23040802)
Science. 2014 Aug 15;345(6198):765-70. (PMID: 25124430)
ACS Chem Neurosci. 2017 Oct 18;8(10):2168-2179. (PMID: 28665105)
Sci Adv. 2019 Nov 20;5(11):eaax3432. (PMID: 31799390)
Neuron. 2021 Dec 15;109(24):4036-4049.e5. (PMID: 34710366)
Neurosci Bull. 2022 Nov;38(11):1277-1291. (PMID: 35788510)
Nature. 2025 Jan;637(8047):974-982. (PMID: 39779849)
Elife. 2016 Oct 22;5:. (PMID: 27770569)
Curr Biol. 2019 Dec 16;29(24):4218-4230.e8. (PMID: 31786064)
Elife. 2015 Sep 30;4:. (PMID: 26422512)
J Neurogenet. 2012 Jun;26(2):238-44. (PMID: 22436011)
iScience. 2023 Feb 02;26(3):106123. (PMID: 36876123)
Nat Genet. 2007 May;39(5):678-82. (PMID: 17450142)
Neurochem Res. 2019 Jun;44(6):1508-1516. (PMID: 30661229)
J Indian Inst Sci. 2017 Dec;97(4):511-525. (PMID: 29657367)
Int J Mol Sci. 2023 Feb 23;24(5):. (PMID: 36901836)
Curr Biol. 2012 Jul 10;22(13):1155-65. (PMID: 22658598)
Elife. 2020 Nov 03;9:. (PMID: 33141021)
Nat Commun. 2021 Apr 6;12(1):2044. (PMID: 33824330)
J Neurosci. 2015 Sep 16;35(37):12792-812. (PMID: 26377467)
PLoS One. 2016 Dec 9;11(12):e0167518. (PMID: 27936023)
Cell Tissue Res. 2021 Jan;383(1):473-483. (PMID: 33515294)
Grant Information: 1231556 Fondo Nacional de Desarrollo Científico y Tecnológico
Contributed Indexing: Keywords: Drosophila females; locomotor activity; post-mating behavioral response; serotonergic system; social interaction
Substance Nomenclature: 333DO1RDJY (Serotonin)
Entry Date(s): Date Created: 20260827 Date Completed: 20260827 Latest Revision: 20260829
Update Code: 20260829
PubMed Central ID: PMC13512695
DOI: 10.3390/ijms27167070
PMID: 42653077
Βάση Δεδομένων: MEDLINE
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  Data: Mating-Induced Behavioral Changes in Female Drosophila Are Modulated by Serotonin.
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  Data: <searchLink fieldCode="AU" term="%22Guerra-Ayala+S%22">Guerra-Ayala S</searchLink>; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.<br /><searchLink fieldCode="AU" term="%22Cortés-Henott+A%22">Cortés-Henott A</searchLink>; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.<br /><searchLink fieldCode="AU" term="%22Amado-Hinojosa+P%22">Amado-Hinojosa P</searchLink>; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.<br /><searchLink fieldCode="AU" term="%22González-Teuber+M%22">González-Teuber M</searchLink>; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.<br /><searchLink fieldCode="AU" term="%22Gajardo+I%22">Gajardo I</searchLink>; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.<br /><searchLink fieldCode="AU" term="%22Campusano+JM%22">Campusano JM</searchLink>; Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8331150, Chile.
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– Name: Abstract
  Label: Abstract
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  Data: Mating influences the physiology of female animals and induces changes in behaviors associated with securing resources for their offspring. Modulatory neurotransmitters are known to adjust behavioral responses of animals according to environmental cues and internal physiological states. In that regard, it has been shown that serotonin (5-HT) modulates some post-mating behavioral features in Drosophila. However, it remains unknown whether serotonin regulates post-mating social behavior or responses to food cues in flies, which are among several behaviors that serve to secure their survival and reproduction. Here, we report a behavioral paradigm, the yeast-response test (YRT), for exploring locomotor and social behavioral changes in groups of virgin and mated females using yeast odor as a naturalistic food signal. Using this setup, we found that exposure to yeast odor increases social interactions in mated but not virgin females under non-aversive conditions and that these responses are suppressed at aversive temperatures. Furthermore, chronic impairment of serotonin transporter function reverses the social behavioral response to food odor in mated females, without affecting their olfactory capacity. Whole-brain 5-HT content, as well as histone serotonylation, were unchanged between virgin and mated females, suggesting that 5-HT contribution to these changes is mediated at a circuit level rather than by global neuromodulatory mechanisms. Consistent with this idea, silencing serotonin projection neurons (SPNs) selectively abolished mating-induced changes in social behavior without affecting the locomotor effects. These results provide further support for data in the literature showing that SPNs belong to a discrete circuit underlying social behaviors in female flies. Together, these results support a model in which serotonergic signaling shapes post-mating behavior in Drosophila through functionally segregated neural circuits.
– Name: Ref
  Label: References
  Group: RefInfo
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  Data: <i>Keywords: </i>Drosophila females; locomotor activity; post-mating behavioral response; serotonergic system; social interaction
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        Text: English
    Subjects:
      – SubjectFull: Brain metabolism
        Type: general
      – SubjectFull: Serotonergic Neurons metabolism
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      – SubjectFull: Animals
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      – SubjectFull: Drosophila melanogaster physiology
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