Academic Journal
View in EDS

Standardized Workflow for High-Content Imaging and Data Processing.

Bibliographic Details
Title: Standardized Workflow for High-Content Imaging and Data Processing.
Authors: Liu X; College of Medical Technology, Chengdu University of Traditional Chinese Medicine., Wang Y; Innovative Institute of Chinese Medicine and Pharmacy/Institute of Interdisciplinary Studies, Chengdu University of Traditional Chinese Medicine; 20152056@cdutcm.edu.cn., Tan Z; School of Life Science and Technology, Southeast University.
Source: Journal of visualized experiments : JoVE [J Vis Exp] 2026 Aug 21 (234). Date of Electronic Publication: 2026 Aug 21.
Publication Type: Journal Article; Video-Audio Media; Research Support, Non-U.S. Gov't
Language: English
Journal Info: Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE
Imprint Name(s): Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]-
MeSH Terms: Image Processing, Computer-Assisted*/methods , Image Processing, Computer-Assisted*/standards, Benzimidazoles/chemistry ; Staining and Labeling/methods ; Staining and Labeling/standards ; Animals ; Mice ; Workflow
Abstract: To address the challenges of low reproducibility due to operational variations in scientific research imaging and the complexity of analyzing a large number of samples using conventional methods, we introduce a protocol for staining HT22 cells with PI and Hoechst 33342 and then imaging and analyzing the data using the High-Content Imaging System. This workflow aims to establish robust imaging setting parameters and stringent quality control standards. It demonstrates the instrument's imaging settings and the workflow for three different image processing methods provided by the instrument: Multi-Wavelength Cell Scoring (Multi), Live-Dead (Live), and Custom Module Editor (CME). The image processing methods are compared and validated against the traditional method, ImageJ, to ensure their effectiveness. The study ultimately summarizes a workflow for using the High-Content Imaging System to determine cell viability using the PI and Hoechst double-staining method. By standardizing the entire imaging and data processing workflow, the study significantly enhances the reproducibility and reliability of scientific research imaging, thereby providing solid methodological support for cross-laboratory data comparison and advancing scientific research.
Substance Nomenclature: P976261J69 (bisbenzimide ethoxide trihydrochloride)
0 (Benzimidazoles)
Entry Date(s): Date Created: 20260822 Date Completed: 20260822 Latest Revision: 20260907
Update Code: 20260907
DOI: 10.3791/71863
PMID: 42631612
Database: MEDLINE
FullText Text:
  Availability: 0
Header DbId: cmedm
DbLabel: MEDLINE
An: 42631612
AccessLevel: 3
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Standardized Workflow for High-Content Imaging and Data Processing.
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AU" term="%22Liu+X%22">Liu X</searchLink>; College of Medical Technology, Chengdu University of Traditional Chinese Medicine.<br /><searchLink fieldCode="AU" term="%22Wang+Y%22">Wang Y</searchLink>; Innovative Institute of Chinese Medicine and Pharmacy/Institute of Interdisciplinary Studies, Chengdu University of Traditional Chinese Medicine; 20152056@cdutcm.edu.cn.<br /><searchLink fieldCode="AU" term="%22Tan+Z%22">Tan Z</searchLink>; School of Life Science and Technology, Southeast University.
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22101313252%22">Journal of visualized experiments : JoVE</searchLink> [J Vis Exp] 2026 Aug 21 (234). <i>Date of Electronic Publication: </i>2026 Aug 21.
– Name: TypePub
  Label: Publication Type
  Group: TypPub
  Data: Journal Article; Video-Audio Media; Research Support, Non-U.S. Gov't
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: TitleSource
  Label: Journal Info
  Group: Src
  Data: <i>Publisher: </i><searchLink fieldCode="PB" term="%22MYJoVE+Corporation%22">MYJoVE Corporation </searchLink><i>Country of Publication: </i>United States <i>NLM ID: </i>101313252 <i>Publication Model: </i>Electronic <i>Cited Medium: </i>Internet <i>ISSN: </i>1940-087X (Electronic) <i>Linking ISSN: </i><searchLink fieldCode="IS" term="%221940087X%22">1940087X </searchLink><i>NLM ISO Abbreviation: </i>J Vis Exp <i>Subsets: </i>MEDLINE
– Name: PublisherInfo
  Label: Imprint Name(s)
  Group: PubInfo
  Data: <i>Original Publication</i>: [Boston, Mass. : MYJoVE Corporation, 2006]-
– Name: SubjectMESH
  Label: MeSH Terms
  Group: Su
  Data: <searchLink fieldCode="MM" term="%22Image+Processing%2C+Computer-Assisted%22">Image Processing, Computer-Assisted*</searchLink>/<searchLink fieldCode="MM" term="%22Image+Processing%2C+Computer-Assisted+methods%22">methods</searchLink> <br /><searchLink fieldCode="MM" term="%22Image+Processing%2C+Computer-Assisted%22">Image Processing, Computer-Assisted*</searchLink>/<searchLink fieldCode="MM" term="%22Image+Processing%2C+Computer-Assisted+standards%22">standards</searchLink><br /><searchLink fieldCode="MH" term="%22Benzimidazoles%22">Benzimidazoles</searchLink>/<searchLink fieldCode="MH" term="%22Benzimidazoles+chemistry%22">chemistry</searchLink> ; <searchLink fieldCode="MH" term="%22Staining+and+Labeling%22">Staining and Labeling</searchLink>/<searchLink fieldCode="MH" term="%22Staining+and+Labeling+methods%22">methods</searchLink> ; <searchLink fieldCode="MH" term="%22Staining+and+Labeling%22">Staining and Labeling</searchLink>/<searchLink fieldCode="MH" term="%22Staining+and+Labeling+standards%22">standards</searchLink> ; <searchLink fieldCode="MH" term="%22Animals%22">Animals</searchLink> ; <searchLink fieldCode="MH" term="%22Mice%22">Mice</searchLink> ; <searchLink fieldCode="MH" term="%22Workflow%22">Workflow</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: To address the challenges of low reproducibility due to operational variations in scientific research imaging and the complexity of analyzing a large number of samples using conventional methods, we introduce a protocol for staining HT22 cells with PI and Hoechst 33342 and then imaging and analyzing the data using the High-Content Imaging System. This workflow aims to establish robust imaging setting parameters and stringent quality control standards. It demonstrates the instrument's imaging settings and the workflow for three different image processing methods provided by the instrument: Multi-Wavelength Cell Scoring (Multi), Live-Dead (Live), and Custom Module Editor (CME). The image processing methods are compared and validated against the traditional method, ImageJ, to ensure their effectiveness. The study ultimately summarizes a workflow for using the High-Content Imaging System to determine cell viability using the PI and Hoechst double-staining method. By standardizing the entire imaging and data processing workflow, the study significantly enhances the reproducibility and reliability of scientific research imaging, thereby providing solid methodological support for cross-laboratory data comparison and advancing scientific research.
– Name: NumberCAS
  Label: Substance Nomenclature
  Group: ID
  Data: P976261J69 (bisbenzimide ethoxide trihydrochloride)<br />0 (Benzimidazoles)
– Name: DateEntry
  Label: Entry Date(s)
  Group: Date
  Data: <i>Date Created: </i>20260822 <i>Date Completed: </i>20260822 <i>Latest Revision: </i>20260907
– Name: DateUpdate
  Label: Update Code
  Group: Date
  Data: 20260907
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.3791/71863
– Name: AN
  Label: PMID
  Group: ID
  Data: 42631612
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42631612
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.3791/71863
    Languages:
      – Code: eng
        Text: English
    Subjects:
      – SubjectFull: Benzimidazoles chemistry
        Type: general
      – SubjectFull: Staining and Labeling methods
        Type: general
      – SubjectFull: Staining and Labeling standards
        Type: general
      – SubjectFull: Animals
        Type: general
      – SubjectFull: Mice
        Type: general
      – SubjectFull: Workflow
        Type: general
      – SubjectFull: Image Processing, Computer-Assisted methods
        Type: general
      – SubjectFull: Image Processing, Computer-Assisted standards
        Type: general
    Titles:
      – TitleFull: Standardized Workflow for High-Content Imaging and Data Processing.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Liu X
      – PersonEntity:
          Name:
            NameFull: Wang Y
      – PersonEntity:
          Name:
            NameFull: Tan Z
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 21
              M: 08
              Text: 2026 Aug 21
              Type: published
              Y: 2026
          Identifiers:
            – Type: issn-electronic
              Value: 1940-087X
          Numbering:
            – Type: issue
              Value: 234
          Titles:
            – TitleFull: Journal of visualized experiments : JoVE
              Type: main
ResultId 1