Academic Journal
Synergistic Effects of Secretome and Surgical Angiogenesis in Acellular Nerve Allograft-Mediated Peripheral Nerve Repair in a Rat Model.
| Τίτλος: | Synergistic Effects of Secretome and Surgical Angiogenesis in Acellular Nerve Allograft-Mediated Peripheral Nerve Repair in a Rat Model. |
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| Συγγραφείς: | Eker O; Department of Plastic, Reconstructive and Aesthetic Surgery, Selcuk University Faculty of Medicine, Konya, Turkey., Yıldıran G; Department of Plastic, Reconstructive and Aesthetic Surgery, Selcuk University Faculty of Medicine, Konya, Turkey., Çelik ZE; Department of Pathology, Selcuk University Faculty of Medicine, Konya, Turkey., Çiçek G; Department of Histology and Embryology, Necmettin Erbakan University Faculty of Medicine, Konya, Turkey., Bağcı FÖ; Department of Histology and Embryology, Necmettin Erbakan University Faculty of Medicine, Konya, Turkey., Akdeniz H; Department of Plastic, Reconstructive and Aesthetic Surgery, Selcuk University Faculty of Medicine, Konya, Turkey., Tosun Z; Department of Plastic, Reconstructive and Aesthetic Surgery, Selcuk University Faculty of Medicine, Konya, Turkey. |
| Πηγή: | Annals of plastic surgery [Ann Plast Surg] 2026 Oct 01; Vol. 97 (4), pp. 461-469. Date of Electronic Publication: 2026 Aug 20. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Little, Brown And Company Country of Publication: United States NLM ID: 7805336 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1536-3708 (Electronic) Linking ISSN: 01487043 NLM ISO Abbreviation: Ann Plast Surg Subsets: MEDLINE |
| Imprint Name(s): | Publication: Boston Ma : Little, Brown And Company Original Publication: Boston, Little, Brown. |
| Ιατρικοί όροι (MeSH): | Nerve Regeneration*/physiology , Sciatic Nerve*/injuries , Sciatic Nerve*/transplantation , Sciatic Nerve*/surgery , Peripheral Nerve Injuries*/surgery , Mesenchymal Stem Cell Transplantation*/methods , Secretome* , Neovascularization, Physiologic*, Animals ; Male ; Rats, Wistar ; Rats ; Allografts ; Disease Models, Animal ; Random Allocation ; Recovery of Function |
| Περίληψη: | Background: Critical-sized peripheral nerve defects remain a major reconstructive challenge. Although autografts are considered the gold standard, their use is limited by donor-site morbidity and graft availability. Acellular nerve allografts (ANAs) offer a promising alternative but are often hampered by inadequate revascularization and limited functional outcomes in large defects. This study investigates whether combining mesenchymal stem cell-derived secretome therapy with surgical angiogenesis can enhance the regenerative capacity of ANAs. Methods: Forty male Wistar rats were randomly assigned to 5 groups (n=8): group 1 (reversed autograft), group 2 (ANA), group 3 (ANA+secretome), group 4 (ANA+surgical angiogenesis), and group 5 (ANA+secretome+surgical angiogenesis). A 10 mm sciatic nerve defect was created in all animals. Functional recovery was evaluated using the Sciatic Functional Index (SFI) at 6 and 9 weeks. Electrophysiological, histologic, immunohistochemical (CD34), and muscle weight/volume assessments were performed at 9 weeks. Results: Group 5 demonstrated the highest functional recovery among ANA-based interventions, achieving outcomes comparable to autografts. Groups 4 and 5 showed significantly improved axonal regeneration compared with ANA alone. CD34 immunostaining revealed enhanced vascularization in groups 4 and 5, with levels similar to the autograft group. No significant differences were observed in electrophysiological or muscle mass parameters. Conclusions: The combination of secretome therapy and surgical angiogenesis synergistically enhances the regenerative performance of acellular nerve allografts. This strategy may provide a clinically translatable alternative to autografts, particularly in challenging repair scenarios involving avascular or scarred recipient beds. (Copyright © 2026 Wolters Kluwer Health, LLC. All rights reserved.) |
| Competing Interests: | Conflicts of interest and sources of funding: This study was supported by the Selcuk University Scientific Research Projects Institution. |
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| Contributed Indexing: | Keywords: acellular nerve allograft; mesenchymal stem cell secretome; peripheral nerve regeneration; rat sciatic nerve model; surgical angiogenesis |
| Entry Date(s): | Date Created: 20260820 Date Completed: 20260910 Latest Revision: 20260910 |
| Update Code: | 20260910 |
| DOI: | 10.1097/SAP.0000000000004835 |
| PMID: | 42623489 |
| Βάση Δεδομένων: | MEDLINE |
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